The influence of ethanol on cell membrane fluidity, migration, and invasion of murine melanoma cells

Silberman, Simone; McGarvey, Terence W.; Comrie, Eric; Persky, Bruce

doi:10.1016/0014-4827(90)90257-b

Cited by 24 publications

(13 citation statements)

References 5 publications

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“…We concluded this from results in nonpancreatic cells showing that ethanol influences PKC subcellular distribution and activity [21][22][23][24][25] and that it induces transformations of the actin cytoskeleton [26][27][28]. However, to our knowledge, pancreatic acinar cells have not yet been tested for such effects of ethanol.…”

Section: Introductionmentioning

confidence: 80%

Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells — Comparison with effects of CCK

et al. 2004

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Section: Introductionmentioning

confidence: 80%

Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells — Comparison with effects of CCK

et al. 2004

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“…The K-1735 cells used in this study are highly invasive and highly migratory [32]. The K-1735 cells used in this study are highly invasive and highly migratory [32].…”

Section: Discussionmentioning

confidence: 99%

The effect of butyric acid and retinoic acid on invasion and experimental metastasis of murine melanoma cells

1990

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The effect of butyric acid (BA) and all trans-retinoic acid (RA) on murine melanoma cells was investigated in vitro and in vivo. The in vitro assays included 3H-IdUR incorporation, adhesion, migration and invasion experiments. Butyric acid decreased 3H-IdUR cellular incorporation within 24 h and increased adhesion as measured by trypsin release of 3H-IdUR labelled cells from either polycarbonate (p.c.) or Matrigel-coated p.c. membranes. Migration and invasion rates after 72 h were quantified by scanning electron microscopy (SEM). The invasion barrier consisted of Matrigel-coated p.c. membranes. Butyric acid significantly enhanced migration and invasion of B16a cells, while RA significantly decreased migration and invasion of B16a and K-1735 cells. Subcutaneous administration of either BA or RA pellets significantly decreased the number of lung nodules in the experimental metastatic assay. The experimental metastatic assay is defined as a tail vein inoculation protocol followed by subsequent lung evaluation.

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“…Cell-attached patches We tested the effects of 3% (v/v) ethanol, a concentration that significantly increases the fluidity of membranes (Goldstein, 1986;Silberman et al, 1990), on the behaviour of stretch-activated K channels. The ethanol was applied in the pipette and patches were exposed …”

Section: Ethanolmentioning

confidence: 99%

Pharmacology of stretch‐activated K channels in Lymnaea neurones

Small

Morris

1995

British J Pharmacology

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1 Single-channel recording was used to describe the pharmacology of stretch-activated K channels in Lymnaea neurones using channel blockers amiloride, tetraethylammonium (TEA), quinidine, gadolinium (Gd) and diltiazem. 2 Amiloride, TEA and quinidine applied to the outside face of the membrane all produced a fast flickery block of stretch-activated K channels. All of these agents were without effect when applied at the inside face at concentrations as high as 10, 200 and 10 mM respectively. Neither Gd nor diltiazem had any effect on stretch-activated K channels extracellularly (100 JiM).3 Amiloride, TEA and quinidine block were voltage-independent with IC,0 values at positive (and negative membrane potentials of 2.3 (and 2.0) mM, 48 (and 54) mM and 0.8 (and 0.7) mM respectively. Woodhull plots for TEA and quinidine block confirmed the voltage independence of stretch-activated K channel block by these agents.4 Hill plots of the amilorde, TEA and quinidine block yield Hill coefficients at positive (and negative) membrane potentials of 1.7 (and 1.5), 1.4 (and 1.2) and 1.5 (and 1.6 mM) respectively. 5 Ethanol (3%) had no apparent effect on stretch-activated K channel kinetics or conductance yet reduced the efficacy of quinidine block. 6 The above pharmacological fingerprint of the stretch-activated K channel is discussed with reference to other K-selective and stretch-activated channels.

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The influence of ethanol on cell membrane fluidity, migration, and invasion of murine melanoma cells

Cited by 24 publications

References 5 publications

Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells — Comparison with effects of CCK

Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells — Comparison with effects of CCK

The effect of butyric acid and retinoic acid on invasion and experimental metastasis of murine melanoma cells

Pharmacology of stretch‐activated K channels in Lymnaea neurones

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