The Influence of Explant Types and Orientation on in Vitro Culture

Abstract

Inflorescence, apical and lateral buds of Musa balbisiana 'Kluai Hin' (BBB group) were used to culture on MS medium supplemented with 22 µM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 µM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.