The influence of fatty acids on theophylline binding to human serum albumin. Comparative fluorescence study

Nutrition Therapy Regulates Caffeine Metabolism with Relevance to NAFLD and Induction of Type 3 Diabetes

“…Caffeine can be converted to theophylline in cells but beneficial theophylline effects may be sensitive to toxic elevated caffeine levels in cells. Furthermore delayed caffeine clearance and increased theophylline levels [88][89][90][91][92][93][94] may interfere with magnesium and calcium binding to albumin (Figure 3). Saturation of albumin by caffeine or theophylline interferes with plasma magnesium and calcium corrections [95,96] that are related to plasma albumin contents ( Figure 3).…”

Probing the interaction of human serum albumin with DPPH in the absence and presence of the eight antioxidants

“…Upon excitation at 280 nm, both Trp and Tyr are readily excited, while at an excitation wavelength of 295 nm, only the Trp emits fluorescence [32]. To identify DPPH binding sites on HSA, the fluorescence of HSA excited at 280 nm and 295 nm in the presence of the DPPH were both measured [33]. The plots of F/F 0 against the concentration of DPPH are shown in Fig.…”

Obese Patient-Derived Sera have Proinflammatory Effect

“…Adipocytes are important locations for the production of pro-inflammatory cytokines, and there is a significant positive correlation between body mass index (BMI) and the concentration of plasma tumor necrosis factor-alpha (TNF-α) [15]. The serum of obese patients has long been a focus of both domestic and international studies, such as investigate whether Serum Myeloperoxidase is the Biomarker of Traditional Cardiac Risk Parameters in Obesity [16]; investigating the influence of fatty acids on theophylline binding to human serum albumin obtained from blood from obese patients [17]; the favorable effect of short-term lifestyle intervention on human serum paraoxonase-1 activity and adipokine levels in childhood obesity [18]; the increase in serum levels of fetal antigen 1 (FA1) with obesity; and the negative dependence of the insulin sensitivity index on FA1 levels [19]. However, the pro-inflammatory effects of sera from weightmatched obese patients with different compositions of metabolic disorders and the main underlying pro-inflammatory pathways have not been reported.…”