The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation

Christopherson, Gregory T.; Song, Hongjun; Mao, Hai‐Quan

doi:10.1016/j.biomaterials.2008.10.004

Cited by 703 publications

(569 citation statements)

References 28 publications

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“…This effect can be due to the FAs pattern and lower 3 stress fibres organization presented by SC cultured on micro-fibers substrate and to the mechanical 4 properties of the fibres in according to literature data discussed before [51][52][53]. Our data are in 5 according with a study in which neural stem/progenitor cells displayed fibres higher degree of 6 proliferation and cell spreading and lower degree of cell aggregation as the fibre diameter decreased 7 [23]. 8…”

Section: Increasing Fibre Diameter Size Resulted In Lower Cell Prolifsupporting

confidence: 76%

The influence of electrospun fibre size on Schwann cell behaviour and axonal outgrowth

Gnavi

Fornasari

Tonda‐Turo

et al. 2015

Materials Science and Engineering: C

101

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Fibrous substrates, functioning as a temporary extracellular matrix, can be easily prepared by 5 electrospinning which allows to obtain fibrous matrices suitable as internal filler for nerve guidance 6 channels. In this study, gelatin micro-or nano-fibres have been prepared by electrospinning 7 technique by tuning gelatin concentration and solution flow rate. The influence of gelatin fibre 8 diameter on cell adhesion and proliferation was tested in vitro using Schwann cells (SC) and dorsal 9 root ganglia (DRG) explant cultures. Cell adhesion was evaluated by quantifying cell spreading area, 10 actin cytoskeleton organization and focal adhesion complex formation. Nano-fibres showed to 11 promote cell spreading and actin cytoskeleton organization, resulting in higher cellular adhesion 12 and proliferation rate. Yet, cell migration and motility were quantified by transwell and time lapse 13 assays respectively and results showed that cells cultured on micro-fibres displayed higher motility 14 and migration rate. Finally, DRG axon outgrowth resulted to be higher on micro-fibres. These data 15 suggest that gelatin electrospun fibres topography can be adjusted in order to modulate SC and 16 axons organization and that both nano-and micro-fibres are promising fillers for the design of 17 devices for peripheral nerve repair. 18 19

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Section: Increasing Fibre Diameter Size Resulted In Lower Cell Prolifsupporting

confidence: 76%

The influence of electrospun fibre size on Schwann cell behaviour and axonal outgrowth

Gnavi

Fornasari

Tonda‐Turo

et al. 2015

Materials Science and Engineering: C

101

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“…As dictated in previous studies on neural and vascular cells (Daud et al 2012;Wang et al 2012;Christopherson et al 2009), fibre diameter does affect the viability of cells. Similarly to our results Christopherson et al (2009) and Daud et al (2012) found that when fibre diameter is around 1-1.5 µm cells have a lower proliferative capacity. Although, in the Christophereon et al study the comparison is to nanofibres, our study, similarly to Daud et al demonstrated a comparable result with respect to larger fibres, which may be down to points of attachment.…”

Section: Discussionmentioning

confidence: 63%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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“…These fibers are exceedingly long (km range) compared with their diameters (x), which usually follow a unimodal statistical distribution. The mean and spread of such distributions can be controlled and tweaked via process parameters over a wide range, from a few nanometers to hundreds of microns [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. The fiber diameter x is regarded as the prime controllable design parameter to steer scaffold performance in terms of cell response.…”

Section: Introductionmentioning

confidence: 99%

“…Reportedly, nanoscale fibers, in the absence of beads, were found to accelerate and improve cell adhesion and proliferation in the presence of growth factors with respect to the microscale counterpart -partly owing to the higher specific surface area providing a kinetic boost [18,21]. Nanofibers also appeared to sustain growth factor-induced stem cell differentiation [14,15,17,22,23]. However, dense nanoscale networks are not optimal, as they tend to block cell migration through the thickness and are outperformed by microscale fibers in terms of cell ingrowth and viability [20].…”

Section: Introductionmentioning

confidence: 99%

“…However, for the general case of a 3D complex tissue the design problem is normally more complicated and must account for additional aspects, such as the need to achieve simultaneous vascularization of the engineered tissue [6,7,15,17]. To this end, one single scaffold ought to be capable of accommodating several cell populations seeded at one time, each having specific requirements in terms of fiber width and pore size [22,23]. This explains the pursuit for more advanced multiscale electrospun scaffolds.…”

Section: Introductionmentioning

confidence: 99%

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Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

et al. 2010

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a b s t r a c tA novel (scalable) electrospinning process was developed to fabricate bio-inspired multiscale threedimensional scaffolds endowed with a controlled multimodal distribution of fiber diameters and geared towards soft tissue engineering. The resulting materials finely mingle nano-and microscale fibers together, rather than simply juxtaposing them, as is commonly found in the literature. A detailed proof of concept study was conducted on a simpler bimodal poly(e-caprolactone) (PCL) scaffold with modes of fiber distribution at 600 nm and 3.3 lm. Three conventional unimodal scaffolds with mean diameters of 300 nm and 2.6 and 5.2 lm, respectively, were used as controls to evaluate the new materials. Characterization of the microstructure (i.e. porosity, fiber distribution and pore structure) and mechanical properties (i.e. stiffness, strength and failure mode) indicated that the multimodal scaffold had superior mechanical properties (Young's modulus $40 MPa and strength $1 MPa) in comparison with the controls, despite the large porosity ($90% on average). A biological assessment was conducted with bone marrow stromal cell type (mesenchymal stem cells, mTERT-MSCs). While the new material compared favorably with the controls with respect to cell viability (on the outer surface), it outperformed them in terms of cell colonization within the scaffold. The latter result, which could neither be practically achieved in the controls nor expected based on current models of pore size distribution, demonstrated the greater openness of the pore structure of the bimodal material, which remarkably did not come at the expense of its mechanical properties. Furthermore, nanofibers were seen to form a nanoweb bridging across neighboring microfibers, which boosted cell motility and survival. Lastly, standard adipogenic and osteogenic differentiation tests served to demonstrate that the new scaffold did not hinder the multilineage potential of stem cells.

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The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation

Cited by 703 publications

References 28 publications

The influence of electrospun fibre size on Schwann cell behaviour and axonal outgrowth

The influence of electrospun fibre size on Schwann cell behaviour and axonal outgrowth

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

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