The influence of fluorides on mouse sperm capacitation

Dvořáková-Hortová, Kateřina; Šandera, Martin; Jursova, Michaela; Vašinová, J.; Pěknicová, Jana

doi:10.1016/j.anireprosci.2007.07.015

Cited by 38 publications

(12 citation statements)

References 33 publications

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“…Although fluoride can cross the blood-testis barrier to inhibit testicular spermatogenesis and decrease sperm capacitation (Dvorakova-Hortova et al 2008; Sarkar et al 2006), NaF did not significantly affect DNA methylation in H19 , Rasgrf1 , Line1 and genome methylation during spermatogenesis. Of note, H19 and Peg3 were abnormally methylated in the embryo when NaF-treated male mice sperm were the source of oocyte fertilization.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, excessive fluoride exposure can precipitate various pathological changes in reproductive tissues and in the liver (Gupta et al 2007). Fluoride not only can cross the blood-testis barrier to inhibit testicular spermatogenesis and decrease sperm capacitation (Dvorakova-Hortova et al 2008), but also it can readily cross the placental barrier to directly damage developing mammalian fetuses (Goh and Neff 2003). However, the mechanism of fluorine reproductive toxicity is not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Sodium fluoride disrupts DNA methylation of H19 and Peg3 imprinted genes during the early development of mouse embryo

Zhu

Cheng

et al. 2013

Arch Toxicol

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Sodium fluoride (NaF) is associated with embryonic and fetal development abnormalities, but the mechanism by which this occurs is unclear. DNA methylation, an important epigenetic reprogramming mechanism, is essential for normal embryonic development. Thus, we investigated the effect of NaF on DNA methylation in early mouse embryos, as well as mouse sperm and liver using bisulfite sequencing and ELISA. Data indicate that H19, a paternally imprinted gene, compared to control embryos, was less methylated in 8-cell embryos from pregnant mice treated with NaF (100 mg/l) in drinking water for 48 h. Peg3, a maternally imprinted gene, and the Line1 repeated sequence were similarly methylated in NaF-treated and control embryos. Oral ingestion of NaF for 35 days did not significantly change Line1 and genomic global DNA methylation in the liver. H19, Rasgrf1, Line1, and genomic global DNA methylation were also similar in NaF-treated and control sperm. Female mice mated with NaF-treated male mice (35 days) had less methylated H19, but Peg3 was significantly more methylated. Line1 was similarly methylated in treated 8-cell embryos, compared to control embryos. NaF treatment of male mice before copulation significantly increased the expression of H19 in blastocysts, whereas H19 expression was not detected in 8-cell embryos. Data suggest that NaF may interact directly with the embryo to disrupt the maintenance of normal gene imprinting during pregnancy. Long-term NaF exposure of males may not directly affect DNA methylation of the sperm and liver, but the sperm may signal to early embryos with abnormal gene imprinting.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sodium fluoride disrupts DNA methylation of H19 and Peg3 imprinted genes during the early development of mouse embryo

Zhu

Cheng

et al. 2013

Arch Toxicol

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“…The quality of sperm, their viability, or length of the tail or hook may also be influenced by the impact of toxins in the environment (Dvořáková-Hortová et al 2008). Socio-biological effects in the process of spermatogenesis may also impact the resulting form of sperm (Frynta et al 2009;Ramm & Stockley 2009).…”

Section: Discussionmentioning

confidence: 99%

Changes in the curvature of sperm apical hooks in murine rodents

et al. 2011

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Sperm apical hooks in murine rodents play an important role in sperm competition. Apical hooks are more curved and longer in species with relatively larger testes, that is in species with a higher risk of sperm competition. The sperm can form aggregations, 'trains', that can move faster than individual sperm, thus reaching the egg earlier as was observed in Apodemus sylvaticus. The apical hook plays an important role for train formation. This study focuses on the changes in the curvature of sperm apical hooks during the final stages of spermiogenesis and stages before fertilization (sperm-life span). Apical hook curvatures of field mice (A. agrarius and A. sylvaticus) vary significantly between dormant and active sperm. In contrast, there are no significant differences among the stages in the eastern house mouse. Since there are high ranges of angle values in all stages, the mean angles of apical hook curvature are not appropriate for evaluating risk of sperm competiton. The ranges of angle values point to a level of flexibility of the apical hooks. The lengths of sperm hooks in individual species do not change during particular stages. The length and flexibility of the sperm apical hooks are important for the formation of sperm aggregations, thus these sperm characters indicate the risk of sperm competition and the sperm strategies in murine rodents.

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“…In addition, in vitro exposure of human sperm to fluoride elicits a significant decline in sperm motility (Chinoy and Narayana, ). Furthermore, experimental studies in animals demonstrated that fluoride can not only cross the blood‐testis barrier to interfere with the testicular cell cycle (Huang et al, ), disrupt spermatogenesis in the seminiferous tubules (Susheela and Kumar, ; Pushpalatha et al, ), and interrupt sperm functions such as capacitation, hyperactivation, and acrosome reaction (Dvořáková‐Hortová et al, ; Sánchez Gutiérrez, ; Sun et al, ), but can also readily cross the placental barrier to directly damage developing mammalian fetuses (Goh and Neff, ). Fluoride is well‐identified as a cytotoxic agent and can induce apoptosis in vitro via oxidative stress‐mediated mitochondrial dysfunction which triggers caspase 3 activation (Anuradha et al, ).…”

Section: Introductionmentioning

confidence: 99%

Fluoride impairs oocyte maturation and subsequent embryonic development in mice

Liang

Zhao

Ock

et al. 2015

Environmental Toxicology

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The damage caused by fluorosis is permanent, and has been recognized as a public health problem in a number of regions of the world. Although multiple studies provided evidence that sodium fluoride (NaF) elicits adverse effects on reproductive function, the effect of fluoride on female germ cell development is not well understood. Therefore, the present study aimed at evaluating the effect of fluoride treatments on in vivo maturation and developmental potential of mouse oocytes, in which female ICR mice were treated with a range of doses (0, 30, 60, and 150 mg/L) of NaF. After treatment, mice were superovulated to collect ovulated oocytes. The effects of NaF on oocyte quality, fertilization potential and early embryonic development were evaluated, as well as the underlying mechanisms were primarily investigated. The findings of this study showed that NaF treatment resulted in abnormal spindle configuration, actin cap formation, and cortical granule-free domain formation. Additionally, overexposure of mice to NaF notably reduced ATP production and mitochondrial membrane potential, further influencing in vitro fertilization and subsequent embryonic development. These results indicated that NaF treatment impairs the subsequent embryonic developmental potential of the oocytes. In conclusion, overexposure to fluoride in vivo was associated with a significant disruption of cytoskeletal dynamics and decreased oocyte quality, affecting the oocyte's subsequent fertilization and embryonic development. Results of this study provide a rationale for treating reproductive diseases such as infertility or miscarriage caused by environmental contaminants. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1486-1495, 2016.

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The influence of fluorides on mouse sperm capacitation

Cited by 38 publications

References 33 publications

Sodium fluoride disrupts DNA methylation of H19 and Peg3 imprinted genes during the early development of mouse embryo

Sodium fluoride disrupts DNA methylation of H19 and Peg3 imprinted genes during the early development of mouse embryo

Changes in the curvature of sperm apical hooks in murine rodents

Fluoride impairs oocyte maturation and subsequent embryonic development in mice

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