The Influence of Fungi Isolated from Peach Twigs on the Pathogenicity of Cytospora cincta

Royse, Daniel J.

doi:10.1094/phyto-68-603

Cited by 124 publications

(57 citation statements)

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“…Colony interaction and competition were studied using techniques based upon the methods of Royse & Ries (1978) and Chand & Logan (1984). Each medium was inoculated with a 5 mm diameter disc of antagonist positioned diametrically opposite a 5 mm diameter disc of pathogen.…”

Section: Colony Interaction and Competition In Dual Culturementioning

confidence: 99%

“…Some examples include the use of six agar media to study the interactions between the antagonist Fusarium lateritium (Nees) Link and S. sclerotiorum (Sitepu & Wallace, 1984) and two agar media to observe the effect of a range of antagonists aginst Cytospora cincta Sacc. (Royse & Ries, 1978). Although differences in growth and interactions were observed, the media all contained added nutrients.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Media on Growth and Interactions Between a Range of Soil‐borne Glasshouse Pathogens and Antagonistic Fungi

Whipps¹

1987

New Phytologist

184

119

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SUMMARYThe growth of and interactions between the glasshouse pathogens, Rhizoctonia solani Kiihn, Fusarium oxysporum Schlect., Pyrenochaeta lycopersici Schneider & Gerlach, Phomopsis sclerotioides van Kesteren, Sclerotinia sclerotiorum (Lib.) de Bary and Botrytis cinerea Pers. and the antagonistic fungi, Trichoderma harzianum Rifai, Trichoderma viride Pers., Coniothyrium minitans Campbell, Gliocladium roseum (Link.) Bain and Pythium oligandrum Dreschlgr were examined on tap water agar, soil extract agar and potato dextrose agar. The medium had a significant effect on the growth rates of the fungi as well as the production of, and response to, volatile and non-volatile antibiotic compounds and hyphal interactions. Responses to the media appeared to be related both to inherent properties of the fungi and to their natural ecological behaviour. The significance of in vitro assays in screening systems for biological control agents of soil-borne plant pathogens is discussed.

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Section: Colony Interaction and Competition In Dual Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Media on Growth and Interactions Between a Range of Soil‐borne Glasshouse Pathogens and Antagonistic Fungi

Whipps¹

1987

New Phytologist

184

119

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“…The experiment was entirely at random with three replicates. The percentage inhibition of radial growth I = [(C -T) / C] × 100 of R. solani and the width of the zone of inhibition between both colonies were recorded as described by Royse & Ries (1978). Mode of inhibition was assessed on a scale from 1 to 4, in which 1 = Mycelial growth of R. solani ceased due to overgrowth of T. harzianum, 2 = Partial inhibition of R. solani and interacting fungus but both grow slowly over each other, 3 = Mutual inhibition at a distance <2 mm, 4 = Growth of R. solani inhibited at a distance > 2 mm (Chand & Logan, 1984).…”

Section: Disease Incidencementioning

confidence: 99%

In vitro Antagonistic Activity of Trichoderma harzianum against Rhizoctonia solani The Causative Agent of Potato Black Scurf and Stem Canker

Ibrahim

2017

Egypt. J. Bot.

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IN EGYPT Potato (Solanum tuberosum L.) is considered one of the most important vegetable crops as well as many others countries in the world. It plays an important role in the Egyptian agricultural economy, not for local consumption but also for exportation especially to Europe, Russia and Arabic countries. However, potato crop suffer from more than 40 pests and diseases caused by insects, nematodes, viruses, bacteria and fungi, of which black scurf and stem canker induced by Rhizoctonia solani is probably the most serious disease. The world yield losses caused by R. solani were estimated to 5-15%. R. solani is an unspecialized parasite, survive in soil in the absence of host plant and make itself a very difficult pathogen to manage. Reduction or elimination of soil borne inoculum is the only effective solution to overcome the problem and this may be achieved through the application of various control measures of which fungal antagonists consider among the most important tactic. Although R. solaniis usually controlled through the application of chemicals, the serious ecological and financial toll of this fungus has prompted for research on biopesticides as a viable alternative. Trichoderma spp. were well-known fungi often used for the biological control of crop pests, whose anti-fungal mechanisms include competition for the substrate, antibiosis and/or mycoparasitism. Five isolates of Trichoderma harzianum were tested in vitro for their antagonistic potential against Rhizoctonia solani. Both Trichoderma harzianum and Rhizoctonia solani were identified by molecular and morphological methods. In dual culture of all isolates were found antagonistic to the growth of R. solani. The hyphal interaction studied using light microscopy revealed destructive mycoparasitism of R. solani by T. harzianum. The method of mycoparasitism was sparse to intense coiling of R. solani followed by disintegration, disorganization and death of R. solani mycelium.

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“…b. Coeficiente de la forma, calculado a partir de la diferencia del eje lateral y el eje central (Santamaría et al, 2007). c. Porcentaje de inhibición del crecimiento radial = 100 * ((r1-eje C))/r1 (ecuación 1), donde r1 es el máximo radio de la colonia del hongo patógeno en cualquier dirección en la placa control (Royse and Ries, 1978). d. Porcentaje de inhibición de la zona de crecimiento.…”

Section: Variables Analizadasunclassified

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

Flores-Pacheco

2017

Rev. Científica FAREM-Estelí

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RESUMENLos hongos endófitos, son aquellos que colonizan los tejidos vivos internos de las plantas sin causar efecto significativo en el hospedante. Estos hongos, al ejercer en ocasiones, antagonismo frente a patógenos fúngicos a la planta hospedante, pueden ser una solución para el control de muchas de las enfermedades forestales causadas por otros hongos. Este campo ha sido poco evaluado en pruebas in vitro y en pruebas en vivo. Se utilizaron cinco hongos endófitos y seis hongos patógenos que se enfrentaron en medio de cultivo generalista Papa + Dextrosa +Agar (PDA) en condiciones de oscuridad a 25º C por siete días. Durante este periodo se midieron los ejes centrales y laterales de la colonia. Con estos datos se obtuvo el coeficiente de forma del hongo y se calculó el crecimiento directo, el coeficiente de forma del hongo patógeno frente al endófito respectivo, el porcentaje de inhibición del crecimiento radial y el porcentaje de inhibición de la zona de crecimiento. Se establecieron placas únicamente con el hongo patógeno a manera de grupo control. Los datos se analizaron con un Análisis de Varianza y para los casos estadísticamente significativos se aplicó la prueba de Diferencia Mínima Significativa. Los resultados muestran que los endófitos que tienen una mayor inhibición frente a los hongos patógenos. De esta manera se demuestra un efecto controlador de al menos un endófito sobre uno o más patógenos. Esto sugiere su posible utilidad como Agente de Control Biológico (ACB) ante enfermedades forestales. La reducción del crecimiento de los hongos patógenos ante la presencia de los endófitos, puede deberse a los mecanismos de acción de éstos (competencia y secreción de micotoxinas) frente al patógeno. Se deben realizar ensayos in vivo donde los endófitos se inoculen de acuerdo con su ecología y con un tiempo adecuado para la colonización de la planta.Palabras claves: control biológico, hongos patógenos, sanidad vegetal, antagonismo in vitro.

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The Influence of Fungi Isolated from Peach Twigs on the Pathogenicity of Cytospora cincta

Cited by 124 publications

References 0 publications

Effect of Media on Growth and Interactions Between a Range of Soil‐borne Glasshouse Pathogens and Antagonistic Fungi

Effect of Media on Growth and Interactions Between a Range of Soil‐borne Glasshouse Pathogens and Antagonistic Fungi

In vitro Antagonistic Activity of Trichoderma harzianum against Rhizoctonia solani The Causative Agent of Potato Black Scurf and Stem Canker

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

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