The influence of gene polymorphisms on postprandial triglyceride response after oral fat tolerance test meal in patients with diabetes mellitus

Abstract

Summary Aims We evaluated the influence of CETP (rs5882 and rs708272), APOE (rs7412, rs429358) and LPL (rs328) gene polymorphisms on triglyceride (TG) response to oral fat tolerance test (OFTT) meal in patients with well‐controlled type 2 diabetes mellitus (T2DM). Methods Fifty‐one men underwent OFTT and according to postprandial TG response patients were divided into two subgroups (positive [TG ≥ 220 mg/dL, 31 patients] and negative [TG < 220 mg/dL, 20 patients]). All patients were genotyped, and study variants were detected using polymerase chain reaction (PCR) and restricted fragment length polymorphism (RFLP) analysis. Results Patients with genotype SS of LPL gene compared with genotype SX had more frequently positive response to OFTT (P = .04) and lower high‐density lipoprotein cholesterol (HDL‐C) concentration (P = .03). Patients with positive response to OFTT and genotype SS of LPL gene compared with genotype SX had lower AUC (area under the curve)‐TG, 1744 (368) vs 1887 (807) mg/dL/h, respectively, P = .04. CETP and APOE gene polymorphisms had no influence on postprandial TG response to OFTT. Conclusions In patients with well‐controlled T2DM, LPL but not CETP and APOE gene polymorphisms influenced TG postprandial response. Particularly, S447 allele carriers of LPL gene presented more frequently positive postprandial TG response to OFTT compared with 447X allele carriers. No differences were found between allele carriers of patients with negative response to OFTT in any other studied gene polymorphism.

The influence of LPL S447X variants and obesity on lipid profile, oxidative stress, and the risk of T2DM: A case‐control study

“… 17 Gavra and colleagues, through the study on T2DM, observed that the carriers of the LPL SX genotype had significantly more negative oral fat tolerance test response (TG postprandial). 18 However, the studies of Evans et al 19 and Shahzad et al 10 illustrated that the SX genotype of the LPL S447X was not associated with hyperlipidemia. The LPL S447X polymorphism cannot affect the catalytic activity of the LPL; however, this polymorphism might result in higher concentration and the activity of the LPL enzyme.…”

“…Furthermore, TG levels were higher at baseline and postprandially in individuals with T2DM 17 . Gavra and colleagues, through the study on T2DM, observed that the carriers of the LPL SX genotype had significantly more negative oral fat tolerance test response (TG postprandial) 18 . However, the studies of Evans et al 19 and Shahzad et al 10 illustrated that the SX genotype of the LPL S447X was not associated with hyperlipidemia.…”