The Influence of Genetic Variation on the Splenic T Cell Cytokine and Specific Serum Antibody Responses to <i>Porphyromonas gingivalis</i> in Mice

Gemmell, E.; Winning, T.; Grieco, D. A.; Bird, P. S.; Seymour, G. J.

doi:10.1902/jop.2000.71.7.1130

Cited by 24 publications

(43 citation statements)

References 21 publications

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“…The results of the present study have demonstrated that in general the percentages of cytokine-positive CD8 cells in the spleens of immunized mice were significantly higher than those of the CD4 cells, as has been reported previously [27,28]. Also, while the percentages of cytokine positive CD8 cells were increased for the most part compared with the control group, only the percentage of IL-4 + CD4 cells was increased in the two groups immunized with both F. nucleatum and P. gingivalis.…”

Section: Discussionsupporting

confidence: 90%

“…Following on from this study, Ebersole et al [19] showed that active immunization of mice with P. gingivalis protected against challenge with P. gingivalis as well as P. gingivalis together with F. nucleatum and this protection in terms of lesion size, correlated with the levels of specific serum IgG antibody. This has also been demonstrated in another study, in which strains of mice demonstrating a faster healing pattern of lesions after challenge with P. gingivalis had higher serum anti-P. gingivalis antibody levels than those strains which had slower healing lesions [27].…”

Section: Discussionsupporting

confidence: 65%

“…The percentage of CD4 and CD8 cells extracted from the spleens staining positive for intracytoplasmic IL-4, IFN-gamma and IL-10 were determined as described previously [26][27][28]. Briefly, surface membrane staining of CD4 and CD8 cells was achieved using phycoerythrin (PE) conjugated rat antimouse CD4 or CD8 (PharMingen, San Diego, CA, USA), followed by fixation of these cells in paraformaldehyde, permeabilization using proteinase K and then incubation with fluorescein isothiocyanate (FITC) conjugated rat antimouse IL-4, IFN-gamma or IL-10 (PharMingen).…”

Section: Flow Cytometric Analysismentioning

confidence: 99%

“…Detection of anti-F. nucleatum and P. gingivalis antibodies Serum samples were assayed for the presence of anti-F. nucleatum and P. gingivalis antibodies using an ELISA technique described by Bird et al [25,27,28]. Briefly, the protein concentrations of both organisms were determined (BCA Protein Assay Kit, Pierce Rockford, IL, USA) and the optimum coating concentration of F. nucleatum and P. gingivalis ascertained to be 5·0 mg/ml protein.…”

Section: Flow Cytometric Analysismentioning

confidence: 99%

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Effect of Fusobacterium nucleatum on the T and B cell responses to Porphyromonas gingivalis in a mouse model

Gemmell

Bird

Carter

et al. 2002

Clinical and Experimental Immunology

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Periodontal disease results from the inflammatory response to bacteria in dental plaque (reviewed in [1]). Although there are well over 300 different bacterial species in the plaque, progression to periodontitis depends not on bacterial load, but on the presence of specific periodontopathic bacteria. The major pathogens identified at the 1996 World Workshop in Periodontics as causative agents are Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Bacteroides forsythus [2]. P. gingivalis has been found in 15% of subjects in an Australian population, the prevalence increasing with increasing pocket depth [3]. In another study, Slots and Ting [4] found that 40-100% of adult periodontitis patients were positive for P. gingivalis. This high occurrence, together with the pathogenic potential of P. gingivalis, has made it a major pathogen of adult periodontitis [5].Immunohistological studies have established that a T cell/macrophage lesion identical to a delayed hypersensitivity 238 Effect of Fusobacterium nucleatum on the T and B cell responses toPorphyromonas gingivalis in a mouse model SUMMARYT cell cytokine profiles and specific serum antibody levels in five groups of BALB/c mice immunized with saline alone, viable Fusobacterium nucleatum ATCC 25586, viable Porphyromonas gingivalis ATCC 33277, F. nucleatum followed by P. gingivalis and P. gingivalis followed by F. nucleatum were determined. Splenic CD4 and CD8 cells were examined for intracytoplasmic interleukin (IL)-4, interferon (IFN)-gamma and IL-10 by dual colour flow cytometry and the levels of serum anti-F. nucleatum and anti-P. gingivalis antibodies determined by an ELISA. Both Th1 and Th2 responses were demonstrated by all groups, and while there were slightly lower percentages of cytokine positive T cells in mice injected with F. nucleatum alone compared with the other groups immunized with bacteria, F. nucleatum had no effect on the T cell production of cytokines induced by P. gingivalis in the two groups immunized with both organisms. However, the percentages of cytokine positive CD8 cells were generally significantly higher than those of the CD4 cells. Mice immunized with F. nucleatum alone had high levels of serum anti-F. nucleatum antibodies with very low levels of P. gingivalis antibodies, whereas mice injected with P. gingivalis alone produced anti-P. gingivalis antibodies predominantly. Although the levels of anti-F. nucleatum antibodies in mice injected with F. nucleatum followed by P. gingivalis were the same as in mice immunized with F. nucleatum alone, antibody levels to P. gingivalis were very low. In contrast, mice injected with P. gingivalis followed by F. nucleatum produced equal levels of both anti-P. gingivalis and anti-F. nucleatum antibodies, although at lower levels than the other three groups immunized with bacteria, respectively. Anti-Actinobacillus actinomycetemcomitans, Bacteroides forsythus and Prevotella intermedia serum antibody levels were also determined and found to be negligible. In conclusion, F. nucleatum ...

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 65%

Section: Flow Cytometric Analysismentioning

confidence: 99%

Section: Flow Cytometric Analysismentioning

confidence: 99%

See 2 more Smart Citations

Effect of Fusobacterium nucleatum on the T and B cell responses to Porphyromonas gingivalis in a mouse model

Gemmell

Bird

Carter

et al. 2002

Clinical and Experimental Immunology

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“…They have roles in the function and differentiation of T cells, and they designate the T-helper subsets to amplify or control the amplitude of the inflammation and the destruction related to inflammation (2,3). The macrophages and T cells are dominate in the early/stable lesions of chronic periodontitis (CP), leading to the consideration that this response is developed by the Th1 cytokines.…”

Section: Introductionmentioning

confidence: 99%

Serum leptin levels and their proportional relationship with pro- and antiinflammatory mediators in aggressive periodontitis: preliminary report

Ay¹,

Sütçü²,

Koçak³

et al. 2013

Turk J Med Sci

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In this study, some 2,3,6,8-tetraarylimidazo[1,2-a]pyrazine derivatives were synthesized by reacting 1-(2aryl-2-oxoethyl)-2-aryloyl-4,5-diarylimidazoles from 2-aryloyl-4,5-diarylimidazole and 2-bromoacetophenone derivatives with ammonium acetate in acetic acid by using the method that was previously developed and repeatedly tested in our studies. Structural elucidation of the compounds was performed by IR, 1 H-NMR, and MASS spectroscopic data and elemental analysis results. Anticancer activities of selected compounds were evaluated and the noticeable activity values were reported.

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Genetic susceptibility to chronic periodontal disease

Baker

Roopenian

2002

Microbes and Infection

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The Influence of Genetic Variation on the Splenic T Cell Cytokine and Specific Serum Antibody Responses to Porphyromonas gingivalis in Mice

Cited by 24 publications

References 21 publications

Effect of Fusobacterium nucleatum on the T and B cell responses to Porphyromonas gingivalis in a mouse model

Effect of Fusobacterium nucleatum on the T and B cell responses to Porphyromonas gingivalis in a mouse model

Serum leptin levels and their proportional relationship with pro- and antiinflammatory mediators in aggressive periodontitis: preliminary report

Genetic susceptibility to chronic periodontal disease

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