The influence of gut microbiota on the rheological characterization of soy hull polysaccharide and mucin interactions

Yang, Ling; Huang, Jinghang; Luo, Mingshuo; Wang, Ziyi; Zhu, Li; Wang, Shengnan; Zhu, Danshi; Li, He

doi:10.1039/c9ra09594b

Cited by 19 publications

(5 citation statements)

References 45 publications

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“…Intestinal flora, as an indispensable component of feces, plays an important role in TAG metabolism. Previous studies have indicated that a wide variety of microbes reside in the gastrointestinal tract of healthy individuals . These bacteria comprise the gut microbiota, which is a large and complex ecosystem, with more than 400 species of bacteria in the human colon. , Many studies have shown that the intestinal microbiota is an important part of the intestinal microecosystem and plays a crucial role in physiological functions and processes, such as nutrient absorption, growth, development, and biological barriers in the body .…”

Section: Resultsmentioning

confidence: 99%

Study on the Biotransformation and Activities of Astragalosides from Astragali Radix In Vitro and In Vivo

Song,

Li,

Liu

et al. 2023

J. Agric. Food Chem.

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Astragalosides (AGs), as one of the main active ingredients in Astragali Radix (AR), have a series of biological activities. Previous studies have only qualitatively identified the metabolites of AGs in AR, resulting in a lack of quantification. In the present study, the original material was selected from 12 origins based on the levels of 4 AGs by high-performance liquid chromatography (HPLC). The prototype components and metabolites of total AGs (TAGs) in feces, urine, and plasma samples of rats were thoroughly screened and characterized by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). The fermentation reaction and metabolites were verified by human fecal TAG fermentation in vitro. The metabolites of AG I, II, and IV transformed by human feces at different times were identified using UHPLC-HRMS, and the partial metabolites were quantified by HPLC. Furthermore, the anti-inflammatory and antioxidant activities of the metabolites were evaluated based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells in vitro. In total, 13 AGs and 170 metabolites were identified in TAGs as well as in the plasma, urine, and feces of Sprague-Dawley (SD) rats by UHPLC-HRMS, including 28, 36, and 170 metabolites in the plasma, urine, and feces, respectively. The metabolites included the products of deglycosylation, demethylation, hydroxylation, glucuronidation, sulfation, and cysteine-binding reactions. Moreover, the TAG fermentation results in vitro showed great similarity. The human fecal incubation experiments for AG I, II, and IV demonstrated that the metabolic reaction of TAGs mainly occurred in intestinal feces and that deglycosylation, demethylation, and hydroxylation were the main pathways of their metabolism. HPLC quantitative analysis of the transformation solution at different time points showed that AGs were transformed into secondary glycosides [cycloastragenol-6-glucoside (CAG-6-glucoside)] and aglycones [cycloastragenol (CAG)] through a deglycosylation reaction. Analysis of the pharmacological activity showed that the anti-inflammatory and antioxidant activities of the metabolites were associated with the levels of the corresponding aglycones. Further, metabolic profiles of the TAGs were constructed. Overall, this study revealed the metabolic process of AGs in the intestine, providing guidance for the metabolism and pharmacological effects of other saponins.

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Section: Resultsmentioning

confidence: 99%

Study on the Biotransformation and Activities of Astragalosides from Astragali Radix In Vitro and In Vivo

Song,

Li,

Liu

et al. 2023

J. Agric. Food Chem.

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“…There is also the presence of pectin (4.2%) and protein (9.4%) 1,2 . It has been confirmed that soy hull polysaccharide (SHP) has excellent biological activity and a new type of natural dietary fiber energy 3 . Recently, Han et al 4 .…”

Section: Introductionmentioning

confidence: 99%

“…1,2 It has been confirmed that soy hull polysaccharide (SHP) has excellent biological activity and a new type of natural dietary fiber energy. 3 Recently, Han et al 4 indicated that SHP has the potential to be developed as a new functional food contributing to the alleviation of liver damage. Yet at the same time, since SHP is rich in hydroxyl groups and has a small amount of protein, our previous studies demonstrated that it had good emulsification, thickening, and gelation properties.…”

Section: Introductionmentioning

confidence: 99%

Composition, morphology, interfacial rheology and emulsifying ability of soy hull polysaccharides extracted with ammonium oxalate and sodium citrate as extractants

et al. 2023

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BACKGROUND Soy hull, a by‐product of crop processing, is rich in pectin‐like polysaccharides that have potential for thickening, gelling and emulsifying applications. The effect of ammonium oxalate (SHPA) and sodium citrate (SHPS) on the conformation, physicochemical properties and emulsifying ability of soy hull polysaccharide (SHP) were investigated. RESULTS The composition analysis showed that SHPS had more polysaccharide, protein, and higher molecular weight than SHPA. Images of atomic force microscopy (AFM) and scanning electron microscopy (SEM) showed that SHPS molecules appeared spherical bodies with smooth and firm surfaces, while SHPA molecules appeared chain‐like bodies with rough and wrinkled surface. At the oil–water interface, SHPS adsorbed faster and formed a more elastic interfacial layer than SHPA. The characterization of the prepared emulsions showed that the SHPS emulsion was a smaller particle size and more stable system within 30 days than SHPA emulsion, especially at the SHPS concentration of 9 mg mL−1. Images of cryo‐scanning electron microscopy (cryo‐SEM) also demonstrated SHPS formed clearer network structure on the surface of the oil droplets, compared to SHPA. CONCLUSION Overall, ammonium oxalate and sodium citrate significantly influenced the composition and properties of the SHP. SHPS exhibited a better emulsifying ability than SHPA, which was mainly due to the higher protein content of SHPS and the sodium ion (Na+) residue of sodium citrate. This study is useful for the extraction and application of SHP and other plant‐based polysaccharides. © 2023 Society of Chemical Industry.

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“…1 The interaction between polysaccharides fermented by intestinal microbes and IM has received extensive attention. 2 IM—a glycoprotein network that exists on the membrane surface of intestinal epithelial cells—consists mainly of water (95% w/w) and mucin (about 5% w/v). 3 Mucin is a high molecular weight glycoprotein formed and released by goblet cells, which consume glutamine and a large number of other amino acids.…”

Section: Introductionmentioning

confidence: 99%

Interactions between intestinal microbial fermentation products of Pleurotus eryngii polysaccharide with gut mucus

Ma,

et al. 2024

Food Funct.

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The influence of gut microbiota on the rheological characterization of soy hull polysaccharide and mucin interactions

Abstract: SSHP increased the viscoelasticity of the interfacial film and reduced the interfacial tension at the air–water interface by interacting with mucin. SSHP increased the ratio of Proteobacteria and Firmicutes and promoted the growth of probiotics.

Cited by 19 publications

References 45 publications

Study on the Biotransformation and Activities of Astragalosides from Astragali Radix In Vitro and In Vivo

Study on the Biotransformation and Activities of Astragalosides from Astragali Radix In Vitro and In Vivo

Composition, morphology, interfacial rheology and emulsifying ability of soy hull polysaccharides extracted with ammonium oxalate and sodium citrate as extractants

Interactions between intestinal microbial fermentation products of Pleurotus eryngii polysaccharide with gut mucus

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