The influence of head-space and inoculum dilution on in vitro ruminal methane measurements

Longo, C.; Bueno, Ives Cláudio da Silva; Nozella, E.F.; Goddoy, P B; Filho, S.L.S. Cabral; Abdalla, Adibe Luiz

doi:10.1016/j.ics.2006.03.017

Cited by 52 publications

(36 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ground samples (0.3 g) were incubated in 45 ml of diluted rumen fluid [37]. Once filled, all the bottles were closed with rubber stoppers shaken and placed in the incubator at 39°C.…”

Section: Methane Production Determinationmentioning

confidence: 99%

Effect of Magnetic Water on the Performance of Lactating Goats

MH¹

2015

JDVAR

View full text Add to dashboard Cite

SummaryThis work was aimed to test the responses of zaraibi goats consumed magnetic water (field of ≈1200 and 3600 gauss) on rumen fermentation, milk and labneh yield and their components. Blood picture and antioxidant status were also studied. Fifteen lactating Zaraibi does (post weaning) were randomly divided into three equal groups (5 does each). First group, were drank tap water and regarded as control group, second and third groups were drank magnetic treated water with 1200 and 3600 gauss, respectively. While nine zaraibi bucks were used in three digestion trials. For rumen fermentation trials three does fitted with permanent rumen fistula were used. All groups of goats were provided free access to the water and fed the same ration [1]. Results indicated that:i. There was an improvement in water quality when exposed to the magnetic field;ii. Magnetic water significantly increased feeding values, nitrogen utilization, VFA's concentration, ruminal bacteria numbers and their activities and microbial protein (MP) syntheses, the highest values were recorded at the used level of 3600 gauss;iii. pH value, ammonia-N concentration and methane production was decreased with magnetic water;iv. DM and NDF with magnetic water were more effective degradability, while no significant different in CP degradability among groups;v. Third group (T2) had significantly increased feed intake and water consumption; it had significantly yielded more milk, 4% FCM, milk fat and protein than other groups;vi. Chemical composition and organoleptic properties of labneh were increased with magnetic groups;vii. some hematological and biochemical (glucose, total protein, albumin and globulin) parameters were significantly increased with magnetized water, while cholesterol and urea-N concentrations, AST and ALT were significantly decreased and viii. Magnetic water resulted in higher antioxidant compared to that drank unmagnetic water.In conclusion, magnetic treatment could improve water quality and consumption, feeding value, ruminal fermentation, blood picture, antioxidant status and hence milk and labneh yields especially with 3600 gauss.

show abstract

“…Ground samples (0.3 g) were incubated in 45 ml of diluted rumen fluid [37]. Once filled, all the bottles were closed with rubber stoppers shaken and placed in the incubator at 39°C.…”

Section: Methane Production Determinationmentioning

confidence: 99%

Effect of Magnetic Water on the Performance of Lactating Goats

MH¹

2015

JDVAR

View full text Add to dashboard Cite

show abstract

“…Helium at 10 ml/min was used as the carrier gas. The methane concentration was determined according to Longo et al (2006) as follows:…”

Section: In Vitro Gas and Methane Productionmentioning

confidence: 99%

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Soltan¹,

Morsy²,

Sallam³

et al. 2012

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Two experiments in vitro were conducted to evaluate four Egyptian forage legume browses, i.e., leaves of prosopis (Prosopis juliflora), acacia (Acacia saligna), atriplex (Atriplex halimus), and leucaena (Leucaena leucocephala), in comparison with Tifton (Cynodon sp.) grass hay for their gas production, methanogenic potential, and ruminal fermentation using a semi-automatic system for gas production (first experiment) and for ruminal and post ruminal protein degradability (second experiment). Acacia and leucaena showed pronounced methane inhibition compared with Tifton, while prosopis and leucaena decreased the acetate:propionate ratio (P<0.01). Acacia and leucaena presented a lower (P<0.01) ruminal NH 3 -N concentration associated with the decreasing (P<0.01) ruminal protein degradability. Leucaena, however, showed higher (P<0.01) intestinal protein digestibility than acacia. This study suggests that the potential methanogenic properties of leguminous browses may be related not only to tannin content, but also to other factors.

show abstract

“…A adição do nitrato na dieta dos doadores de inóculo deu-se para aumentar as bactérias redutoras de nitrato no líquido ruminal para essas competirem com as metanogênicas (JEYANATHAN et al, 2011).O inóculo de ovinos foi utilizado por ser o modelo animal com o qual o laboratório onde foi desenvolvida a primeira fase trabalhava. Foi utilizada a técnica in vitro de produção de gases (THEODOROU et al, 1994) adaptada ao sistema semiautomático do Laboratório de Nutrição AnimalLana/CENA para avaliação da produção de metano in vitro (BUENO et al, 2005;LONGO et al, 2006), usando um transdutor de pressão e um data logger (Press Data 800, Lana, CENA / USP, Piracicaba, SP, Brasil). Meio grama de cada dieta experimental foi adicionado em saquinho de determinação de FDN (#F57 bags; 50 mmx40mm; 25±10 µm pore size; ANKOM, Tecnology Corporation, Fairport, USA) e incubado em frascos de vidro de 160 mL de volume total e head space de 85mL, com 50mL de meio de incubação (meio tampão THEODOROU descrito em PRESTON, 1994) e 25mL de inóculo.…”

Section: Methodsunclassified

Otimização da pecuária nacional de forma sustentável

Natel

Fausto

Aragão

et al. 2016

Rev. bras. saúde prod. anim.

View full text Add to dashboard Cite

RESUMOO objetivo deste trabalho foi avaliar o uso de nitrato de cálcio em substituição ao farelo de soja em dieta de ruminantes sobre a mitigação do metano entérico visando determinar os custos com a dieta do animal e os custos marginais de abatimento de carbono. O estudo foi realizado em duas fases, na primeira foi avaliado o potencial de mitigação de metano do nitrato por produção de gases in vitro. Os dados de mitigação de metano foram utilizados na segunda fase, que simulou uma propriedade leiteira com três sistemas de alimentação controle, sem nitrato, e sistemas com inclusão de 1,5 e 3% de nitrato na dieta. Foram utilizadas planilhas de cálculo para avaliar os custos e receitas de cada sistema com vendas de crédito de carbono resultantes da redução de metano ao final de 10 anos. O valor presente líquido (VPL) e a taxa interna de retorno (TIR) foram os índices utilizados na avaliação financeira. A venda de crédito de carbono não alterou o VPL, no entanto a inclusão de nitrato a 1,5 e 3% na dieta animal mostrou-se economicamente viável, pois reduziu o valor das dietas (R$ 5,89 e 5,81/vaca/dia, respectivamente) em comparação com a dieta controle (R$ 6,13 /vaca/dia), além de contribuir para redução de gases de efeito estufa. Portanto, a suplementação de nitrato,como fonte de nitrogênio não protéico, em dietas para ruminantes, além de reduz a produção de metano possibilitou uma sensível redução no custo variável da atividade.Palavras-chave: alimentação animal, emissão de gases, retorno econômico, ruminantes SUMMARYThe objective of this work was to evaluate the calcium nitrate utilization in replacing to the soybean meal for ruminants diets under the mitigation of enteric methane aiming to determine the animal diet cost and the marginal costs for carbon rebates. The study was conducted in two stages, the first stage was in vitro gas production were performed in order to measure the enteric methane mitigation potential of the nitrate. The methane mitigation data were used in the second stage, which simulated a dairy farm with three feed systems: control system without nitrate, and systems with inclusion of 1.5 to 3% nitrate in the diet. The spreadsheets were used to evaluate the costs and revenues of each system resulting carbon credit sales of methane reduction in 10 years. The net present value (NPV) and the internal rate of return (IRR) were the indexes used in the financial evaluation.The carbon credit sale did not change the NPV significantly, however the inclusion of nitrate to 1.5 and 3% in animal feed showed economically feasible, because it reduced the diet value (R$5.89 e R$5.81/cow/day, respectively) in comparison to control diet (R$6.13/cow/day) besides contributing to the reduction and greenhouse gases. Therefore, the nitrate supplementation beside reduced the methane production, has the positive effect on the variable cost of the activity.

show abstract

The influence of head-space and inoculum dilution on in vitro ruminal methane measurements

Cited by 52 publications

References 8 publications

Effect of Magnetic Water on the Performance of Lactating Goats

Effect of Magnetic Water on the Performance of Lactating Goats

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Otimização da pecuária nacional de forma sustentável

Contact Info

Product

Resources

About