The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro

Abstract

Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM− and the control (each p ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM− and the control after day 3 and day 7 (each p ≤ 0.05). At day 10, an accelerated wound closure was found for the control compared with BSM+/− (each p < 0.05). The highest proliferation rate was observed for BSM+ on day 3 (p ≤ 0.05) followed by BSM− and the control (each p ≤ 0.05). At day 7, a non-significantly increased proliferation was shown for BSM+ while the control was higher than BSM− (each p < 0.05). The least proliferation activity was observed for BSM− (p < 0.05) at day 10. HA biofunctionalization of the BSMs caused an increased HOB activity and might represent a promising alternative to BSM− in oral bone regeneration.

Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo

“…In recent clinical studies, it has been proven that hyaluronic acid has a positive effect on bone formation [ 14 , 60 ]. In our recent study, evidence was provided that HA activates osteoblasts in vitro [ 6 , 8 ]. In addition to cell activation, in the present study, a positive effect of HA on stimulation on vessel formation was discussed and presented.…”

“…All BSM are processed non-cellular and, consequently, exhibit mainly osteoconductive properties. To overcome the lack of osteoinductive and osteogenic properties, a variety of biofunctionalization possibilities such as the addition of growth factors, autologous platelet concentrates [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ] as well as hyaluronic acid [ 8 , 13 , 14 ] have been studied.…”

“…In a previous work, evidence was provided that a combination of xenogenic BSM with HA enhanced osteoblasts viability, migration, and proliferation in vitro [ 8 ]. Besides osteogenic activity [ 13 ], a number of studies revealed a strong pro-angiogenic activity of high and low molecular weight HA [ 36 ], which is limited to the fragments of 4 to 25 disaccharides in length [ 37 , 38 , 39 , 40 ].…”

Lateral sinus augmentation by using natural bovine bone substitute with hyaluronate

“…For this reason, we used natural bovine bone substitute with hyaluronate (cerabone® plus, botiss biomaterials GmbH, Germany), which combines the well-established material cerabone® with the well-known properties of hyaluronic acid. While the high temperature treatment of cerabone® adds favorable physicochemical properties such as increased hydrophilicity, safety and long term-volume stability, the biofunctionalization with hyaluronic acid increases the cell viability [9], [12]. More specifically, a lateral sinus lift approach was selected for this case.…”

“…Moreover, since many years now the hyaluronic acid finds broad use in dentistry and is used in different indications [11]. Also has been used for a bovine bone substitute biofunctionalization in order to influence the osteoblast activity and improve the bone healing capacity [12], [13]. Here a positive effect of the hyaluronic acid on the bone graft integration and new bone formation was observed.…”