The influence of hyperthermiain vitro on the functions of peritoneal macrophages in mice

Yoshioka, Hiroshi; Koga, Shigemasa; Maeta, Michio; Shimizu, Norio; Hamazoe, Ryuichi; Murakami, Atsunobu

doi:10.1007/bf02470725

Cited by 23 publications

(16 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fever is a physiological reaction when the immune system combats invading microorganisms; therefore, it is intuitively appealing that the proinflammatory reaction in vitro would be more potent if it is done under conditions simulating the in vivo situation. While incubation under both hypothermic temperature (below 37°C) and heat shock temperature (41 to 43°C) conditions have been shown to impair immune cell function (18,27,35,37), a number of studies suggest that incubation at fever-range temperatures (38 to 41°C) can augment the responsiveness of murine and human immune cells compared to that at the conventional 37°C incubation: at fever-range temperatures, stimulated PBMCs have been shown to increase antigen presentation and activity of APCs and to augment T cell responses to mitogen stimulation (5,17,33,38). The production of mainly Th1-specific proinflammatory cytokines, e.g., IFN-␥, tumor necrosis factor alpha, IL-1, and IL-2, has been found to increase with incubation at fever-range temperatures, while the production of others, mainly Th2-specific cytokines such as IL-10, is downregulated (4,5,14,38).…”

Section: Discussionmentioning

confidence: 99%

Incubation of Whole Blood at 39°C Augments Gamma Interferon (IFN-γ)-Induced Protein 10 and IFN-γ Responses to Mycobacterium tuberculosis Antigens

Aabye

Ravn

Johansen

et al. 2011

Clin Vaccine Immunol

View full text Add to dashboard Cite

A rarely challenged dogma in cell-mediated immune (CMI) assays is the incubation temperature, 37°C. Fever augments proinflammatory immune responses in vivo, and the aim of this study was to explore whether incubation at fever-range temperature could increase antigen-specific biomarker responses. We compared CMI responses following incubation of whole blood at 37°C and 39°C. Whole blood was obtained from (i) 34 healthy subjects whose blood was incubated with TB10. Cell-mediated immune (CMI) assays are important research tools used to monitor T cell-dependent immune responses toward antigens from, e.g., pathogens or vaccines.CMI assays rely on generation of an in vitro immune response and quantification of the proinflammatory immune response (26). In clinical medicine, CMI assays are used for the diagnosis of infections that cannot be detected with direct methods (28). An example is the diagnosis of latent infection with Mycobacterium tuberculosis, where either purified peripheral blood mononuclear cells (PBMCs) or whole blood is incubated with M. tuberculosis-specific antigens, followed by quantification of the gamma interferon (IFN-␥) response, hence the name IFN-␥ release assays (IGRAs) (3).A rarely challenged dogma in CMI assays-both in research and in diagnostic medicine-is that the incubation temperature is optimal at 37°C. Fever is a part of the innate immune response in vivo and can aid the immune defense by impairing the viability of pathogens with strict temperature preferences or by enhancing immunological reactions (reviewed in reference 38). Previous studies indicate that fever-range temperature in vivo and in vitro augments antigen presentation and coreceptor expression and skews antigen-presenting cells (APCs) toward activation, in concert with augmented T cell responsiveness and Th1 bias (5,6,17,33).One of the major challenges of CMI assays-and IGRAs, in particular-is achieving a consistent and strong response (i.e., high sensitivity) without generating nonspecific immune activation (i.e., compromising specificity).We and others have demonstrated that, compared to T cell responses, the APCs interacting with the specific T cells generate stronger chemokine and cytokine signals that could enable simpler or more sensitive readouts of CMI assays (reviewed in reference 24). In several studies, the chemokine IFN-␥-induced protein 10 (IP-10) has shown promise as a novel marker for immunodiagnosis of infection with M. tuberculosis in both children and adults (1,13,15,(21)(22)(23)30).In an attempt to further improve CMI assays, we hypothesized that incubation at fever-range temperatures in vitro could lead to an increase in proinflammatory immune responses and potentially an increase in the sensitivity of IGRA and CMI assays. Interleukin-7 (IL-7) is a cytokine that promotes survival of T cells (8, 9). IL-10 is an anti-inflammatory cytokine released by T cells and monocytes/macrophages with immune activation (25). Both IL-7 and blocking of IL-10 have previously been shown to augment IFN-␥ responses to M. tub...

show abstract

Section: Discussionmentioning

confidence: 99%

Incubation of Whole Blood at 39°C Augments Gamma Interferon (IFN-γ)-Induced Protein 10 and IFN-γ Responses to Mycobacterium tuberculosis Antigens

Aabye

Ravn

Johansen

et al. 2011

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…After exposure to heat, the phagocytic ability of the macrophages, as well as cytotoxicity was evaluated. Both macrophage functions were enhanced at temperatures up to 40°C [64]. However, these functions were inhibited at temperatures above 41°C.…”

Section: Thermal Regulation Of Immune Cell Proliferationmentioning

confidence: 95%

Diverse immune mechanisms may contribute to the survival benefit seen in cancer patients receiving hyperthermia

et al. 2009

View full text Add to dashboard Cite

There is increasing documentation of significant survival benefits achieved in cancer patients treated with hyperthermia in combination with radiation and/or chemotherapy. Most evidence collected regarding the mechanisms by which hyperthermia positively influences tumor control has centered on in vitro data showing the ability of heat shock temperatures (usually above 42 degrees C) to result in radio- or chemosensitization. However, these high temperatures are difficult to achieve in vivo, and new thermometry data in patients reveal that much of the tumor and surrounding region is only heated to 40-41 degrees C or less as a result of vascular drainage from the target zone of the heated tumor. Thus, there is now a growing appreciation of a role for mild hyperthermia in the stimulation of various arms of the immune system in contributing to long term protection from tumor growth. Indeed, a review of recent literature suggests the existence of an array of thermally sensitive functions which may exist naturally to help the organism to establish a new "set point" of immune responsiveness during fever. This review summarizes recent literature identifying complex effects of temperature on immune cells and potential cellular mechanisms by which increased temperature may enhance immune surveillance.

show abstract

“…There is a slow enhancement of macrophage cytotoxicity and more effective response to lymphokine activation with heating. However, beyond 43°C, prolonged hyperthermia may damage macrophages and thus stimulate tumor growth [143][144][145][146]. Kupffer cells also induce the production of interferon that augments the liver-associated natural killer cell activity [147].…”

Section: Kupffer Cell Activitymentioning

confidence: 99%

Mechanisms of Focal Heat Destruction of Liver Tumors

Nikfarjam

Muralidharan

Christophi

2005

Journal of Surgical Research

342

245

View full text Add to dashboard Cite

The influence of hyperthermiain vitro on the functions of peritoneal macrophages in mice

Cited by 23 publications

References 9 publications

Incubation of Whole Blood at 39°C Augments Gamma Interferon (IFN-γ)-Induced Protein 10 and IFN-γ Responses to Mycobacterium tuberculosis Antigens

Incubation of Whole Blood at 39°C Augments Gamma Interferon (IFN-γ)-Induced Protein 10 and IFN-γ Responses to Mycobacterium tuberculosis Antigens

Diverse immune mechanisms may contribute to the survival benefit seen in cancer patients receiving hyperthermia

Mechanisms of Focal Heat Destruction of Liver Tumors

Contact Info

Product

Resources

About