The Influence of Hypothermia Hibernation Combined with CO2 Anesthesia on Life and Storage Quality of Large Yellow Croaker (Pseudosciaena crocea)

Abstract

We explore the feasibility of the long-term transportation of live large yellow croakers (Pseudosciaena crocea) using the combined method of CO2 anesthesia and hypothermia hibernation, and its effect on the quality of recovered fish stored at 4 °C. Fish treated with CO2 anesthesia at a 2 ppm/s aeration rate were cooled at 3 °C/h to hibernate survived for 36 h at 8 °C in seawater. This method resulted in better survival rates and time, and a lower operational time than hypothermia hibernation or CO2 anesthesia methods. The results of a blood analysis indicated that the stress experienced by the fish during hibernation was mitigated, but existent after recovery. The drip loss rate of the ordinary muscle of hibernated fish was significantly different from that of the control group at 4 °C, but there was no significant difference in the pH, lactic acid content, and color during early storage. Furthermore, hibernation did not affect springiness and chewiness. Thus, the combination of CO2 anesthesia and hibernation may improve the survival and operation efficiency of fish in long-term transportation. However, this method affects the quality of fish after long-term storage. Thus, hibernated fish should be consumed after appropriate domestication or immediately after recovery.

Clove Oil-Nanostructured Lipid Carriers: A Platform of Herbal Anesthetics in Whiteleg Shrimp (Penaeus vannamei)

“…The concentrations needed to anesthetize crustaceans, however, seem to be relatively higher than those needed for fish [ 31 , 32 ]. Cooling and carbon dioxide are efficient anesthetic methods for crustaceans, but such approaches are expensive and inconvenient for live transport [ 33 ]. In recent years, essential oils such as eugenol and menthol have been used as cheap and safe anesthetics in many crustaceans [ 21 ].…”

Influence of the method of preparation of clove oil emulsion on the anaesthetic effect in fish (on the example of Clarias gariepinus

“…The state of anaesthesia is achieved in various ways, in particular, the effect of low temperatures on the fish body (Olifirenko et al, 2020), or the use of carbon dioxide ( Mitsuda & Yoshizawa, 1991). Sometimes these two methods are combined (Tan et al, 2022). However, the most common method of anaesthesia in fish farming is chemical, using anaesthetic drugs, divided into synthetic (MS-222, novocaine, phenasepam, etc.)…”

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“…The state of anaesthesia is achieved in various ways, in particular, the effect of low temperatures on the fish body (Olifirenko et al, 2020), or the use of carbon dioxide ( Mitsuda & Yoshizawa, 1991). Sometimes these two methods are combined (Tan et al, 2022). However, the most common method of anaesthesia in fish farming is chemical, using anaesthetic drugs, divided into synthetic (MS-222, novocaine, phenasepam, etc.)…”