The Influence of Inorganic Ions on the Heat Stability of a Thermophilic Bacteriophage

Ljunger, Claes; Edebo, Margareta

doi:10.1111/j.1399-3054.1972.tb03598.x

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Positive ions help neutralize negative phosphate charges on sugar molecules (on nucleic acid) that repel each other, thus stabilizing DNA. As a matter of fact, the supplementation of thermophage (e.g., TP-84) growth media with calcium or potassium ions enhances survival/titers by 18-10,000-fold at 65 • C [76,88]. On the contrary, the chelation of these ions with EDTA (ethylenediaminetetraacetic acid) or the addition of phosphate (additional negative ions that promote repulsion) leads to the dissociation of the tail from the head of thermophage TP-84 [89].…”

Section: Features That Confer Thermostability In Extreme Hot Environm...mentioning

confidence: 99%

Potential Applications of Thermophilic Bacteriophages in One Health

Liu

Khezerloo

Tumban

2023

IJMS

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Bacteriophages have a wide range of applications such as combating antibiotic resistance, preventing food contamination for food safety, and as biomarkers to indirectly assess the quality of water. Additionally, bacteriophage components (endolysins and coat proteins) have a lot of applications in food processing, vaccine design, and the delivery of cargo to the body. Therefore, bacteriophages/components have a multitude of applications in human, plant/veterinary, and environmental health (One Health). Despite their versatility, bacteriophage/component use is mostly limited to temperatures within 4–40 °C. This limits their applications (e.g., in food processing conditions, pasteurization, and vaccine design). Advances in thermophilic bacteriophage research have uncovered novel thermophilic endolysins (e.g., ΦGVE2 amidase and MMPphg) that can be used in food processing and in veterinary medicine. The endolysins are thermostable at temperatures > 65 °C and have broad antimicrobial activities. In addition to thermophilic endolysins, enzymes (DNA polymerase and ligases) derived from thermophages have different applications in molecular biology/biotechnology: to generate DNA libraries and develop diagnostics for human and animal pathogens. Furthermore, coat proteins from thermophages are being explored to develop virus-like particle platforms with versatile applications in human and animal health. Overall, bacteriophages, especially those that are thermophilic, have a plethora of applications in One Health.

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Section: Features That Confer Thermostability In Extreme Hot Environm...mentioning

confidence: 99%

Potential Applications of Thermophilic Bacteriophages in One Health

Liu

Khezerloo

Tumban

2023

IJMS

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Bacteriophages of Thermophilic ‘Bacillus Group’ Bacteria—A Systematic Review, 2023 Update

Skowron,

Łubkowska,

Sobolewski

et al. 2024

IJMS

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Bacteriophages associated with thermophiles are gaining increased attention due to their pivotal roles in various biogeochemical and ecological processes, as well as their applications in biotechnology and bionanotechnology. Although thermophages are not suitable for controlling bacterial infections in humans or animals, their individual components, such as enzymes and capsid proteins, can be employed in molecular biology and significantly contribute to the enhancement of human and animal health. Despite their significance, thermophages still remain underrepresented in the known prokaryotic virosphere, primarily due to limited in-depth investigations. However, due to their unique properties, thermophages are currently attracting increasing interest, as evidenced by several newly discovered phages belonging to this group. This review offers an updated compilation of thermophages characterized to date, focusing on species infecting the thermophilic bacilli. Moreover, it presents experimental findings, including novel proteomic data (39 proteins) concerning the model TP-84 bacteriophage, along with the first announcement of 6 recently discovered thermophages infecting Geobacillus thermodenitrificans: PK5.2, PK2.1, NIIg10.1, NIIg2.1, NIIg2.2, and NIIg2.3. This review serves as an update to our previous publication in 2021.

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Bacteriophages of Thermophilic ‘Bacillus Group’ Bacteria—A Review

et al. 2021

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Bacteriophages of thermophiles are of increasing interest owing to their important roles in many biogeochemical, ecological processes and in biotechnology applications, including emerging bionanotechnology. However, due to lack of in-depth investigation, they are underrepresented in the known prokaryotic virosphere. Therefore, there is a considerable potential for the discovery of novel bacteriophage-host systems in various environments: marine and terrestrial hot springs, compost piles, soil, industrial hot waters, among others. This review aims at providing a reference compendium of thermophages characterized thus far, which infect the species of thermophilic ‘Bacillus group’ bacteria, mostly from Geobacillus sp. We have listed 56 thermophages, out of which the majority belong to the Siphoviridae family, others belong to the Myoviridae and Podoviridae families and, apparently, a few belong to the Sphaerolipoviridae, Tectiviridae or Corticoviridae families. All of their genomes are composed of dsDNA, either linear, circular or circularly permuted. Fourteen genomes have been sequenced; their sizes vary greatly from 35,055 bp to an exceptionally large genome of 160,590 bp. We have also included our unpublished data on TP-84, which infects Geobacillus stearothermophilus (G. stearothermophilus). Since the TP-84 genome sequence shows essentially no similarity to any previously characterized bacteriophage, we have defined TP-84 as a new species in the newly proposed genus Tp84virus within the Siphoviridae family. The information summary presented here may be helpful in comparative deciphering of the molecular basis of the thermophages’ biology, biotechnology and in analyzing the environmental aspects of the thermophages’ effect on the thermophile community.

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The Influence of Inorganic Ions on the Heat Stability of a Thermophilic Bacteriophage

Cited by 3 publications

References 10 publications

Potential Applications of Thermophilic Bacteriophages in One Health

Potential Applications of Thermophilic Bacteriophages in One Health

Bacteriophages of Thermophilic ‘Bacillus Group’ Bacteria—A Systematic Review, 2023 Update

Bacteriophages of Thermophilic ‘Bacillus Group’ Bacteria—A Review

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