The influence of light and water mass on bacterial population dynamics in the Amundsen Sea Polynya

Richert, Inga; Dinasquet, Julie; Logares, Ramiro; Riemann, Lasse; Yager, Patricia L.; Wendeberg, Annelie; Bertilsson, Stefan

doi:10.12952/journal.elementa.000044

Cited by 10 publications

(20 citation statements)

References 67 publications

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“…Previous research has shown that these primer pairs were successfully implemented for water research and outperformed other primer pairs for classical and next‐generation sequencing‐based diversity studies (Klindworth et al. ; Connelly, Baer, Cooper, Bronk, & Wawrik, ; Richert et al., ; Savio et al., ; Shan et al., ). “Fusion” primers, required for the 454‐pyrosequencing process, were designed according to the guidelines for 454 GS‐FLX+ Titanium Lib‐L sequencing and contained the Roche 454‐pyrosequencing adapters and a sample‐specific multiplex identifier (MID) sequence in between the adapter and the forward primer for sample‐specific sequence tracking (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Assessing the composition of microbial communities in textile wastewater treatment plants in comparison with municipal wastewater treatment plants

et al. 2016

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It is assumed that microbial communities involved in the biological treatment of different wastewaters having a different chemical composition harbor different microbial populations which are specifically adapted to the environmental stresses encountered in these systems. Yet, little is known about the composition of these microbial communities. Therefore, the aim of this study was to assess the microbial community composition over two seasons (winter and summer) in activated sludge from well‐operating textile wastewater treatment plants (WWTPs) in comparison with municipal WWTPs, and to explain observed differences by environmental variables. 454‐pyrosequencing generated 160 archaeal and 1645 bacterial species‐level Operational Taxonomic Units (OTUs), with lower observed richness in activated sludge from textile WWTPs compared to municipal WWTPs. The bacterial phyla Planctomycetes, Chloroflexi, Chlorobi, and Acidobacteria were more abundant in activated sludge samples from textile WWTPs, together with archaeal members of Thaumarchaeota. Nonmetric multidimensional scaling analysis of the microbial communities showed that microbial communities from textile and municipal WWTPs were significantly different, with a seasonal effect on archaea. Nitrifying and denitrifying bacteria as well as phosphate‐accumulation bacteria were more abundant in municipal WWTPs, while sulfate‐reducing bacteria were almost only detected in textile WWTPs. Additionally, microbial communities from textile WWTPs were more dissimilar than those of municipal WWTPs, possibly due to a wider diversity in environmental stresses to which microbial communities in textile WWTPs are subjected to. High salinity, high organic loads, and a higher water temperature were important potential variables driving the microbial community composition in textile WWTPs. This study provides a general view on the composition of microbial communities in activated sludge of textile WWTPs, and may provide novel insights for identifying key players performing important functions in the purification of textile wastewaters.

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Section: Methodsmentioning

confidence: 99%

Assessing the composition of microbial communities in textile wastewater treatment plants in comparison with municipal wastewater treatment plants

et al. 2016

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“…Determining which of these bacteria-particle interactions dominates, temporally and spatially, will be important for understanding the role of pelagic microbial heterotrophs in the carbon cycling of this region. Further analyses of bacterial community structure in the ASP Richert et al, 2015) may help to resolve the issue, but it is already clear that bacterial taxa known for particle attachment and copiotrophy (consumption of high levels of DOM) dominate in the epipelagic zone of this polynya (Richert et al, 2015).…”

Section: The Role Of Particlesmentioning

confidence: 99%

Pelagic microbial heterotrophy in response to a highly productive bloom of Phaeocystis antarctica in the Amundsen Sea Polynya, Antarctica

Williams

Dupont

Loevenich

et al. 2016

Elementa: Science of the Anthropocene

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Heterotrophic bacteria play a key role in marine carbon cycling, and understanding their activities in polar systems is important for considering climate change impacts there. One goal of the ASPIRE project was to examine the relationship between the phytoplankton bloom and bacterial heterotrophy in the Amundsen Sea Polynya (ASP). Bacterial abundance, production (BP), respiration, growth efficiency, and extracellular enzyme activity (EEA) were compared to nutrient and organic matter inventories, chlorophyll a (Chl a), viral and microzooplankton abundance, and net primary production (NPP). Bacterial production and respiration clearly responded (0.04-4.0 and 10-53 µg C L −1 d −1 , respectively) to the buildup of a massive Phaeocystis antarctica bloom (Chl a: 0.2-22 µg L −1 ), with highest rates observed in the central polynya where Chl a and particulate organic carbon (POC) were greatest. The highest BP rates exceeded those reported for the Ross Sea or any other Antarctic coastal system, yet the BP:NPP ratio (2.1-9.4%) was relatively low. Bacterial respiration was also high, and growth efficiency (2-27%; median = 10%) was similar to oligotrophic systems. Thus, the integrated bacterial carbon demand (0.8-2.8 g C m −2 d −1 ) was a high fraction (25-128%; median = 43%) of NPP during bloom development. During peak bloom, activity was particle-associated: BP and EEA correlated well with POC, and size fractionation experiments showed that the larger size fraction (> 3 µm) accounted for a majority (∼ 75%) of the BP. The community was psychrophilic, with a 5x reduction in BP when warmed to 20°C. In deeper waters, respiration remained relatively high, likely fueled by the significant downward particle flux in the region. A highly active, particle-associated, heterotrophic microbial community clearly responded to the extraordinary phytoplankton bloom in the ASP, likely limiting biological pump efficiency during the early season.

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“…, Richert et al. ). Metagenome data demonstrate that an oligotrophic life strategy is dominant in the ocean's free‐living microbial populations (Lauro et al.…”

Section: Introductionmentioning

confidence: 99%

“…, Richert et al. ) bacterioplankton community. At any given location and time in the environmentally patchy ASP (Yager et al.…”

Section: Introductionmentioning

confidence: 99%

Summer comes to the Southern Ocean: how phytoplankton shape bacterioplankton communities far into the deep dark sea

et al. 2019

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During austral spring and summer, the coastal Antarctic experiences a sharp increase in primary production and a steepening of biotic and abiotic gradients that result from increased solar radiation and retreating sea ice. In one of the largest seasonally ice‐free regions, the Amundsen Sea Polynya, pelagic samples were collected from 15 sites during a massive Phaeocystis antarctica bloom in 2010/2011. Along with a suite of other biotic and abiotic measurements, bacterioplankton were collected and analyzed for community structure by pyrosequencing of the 16S rRNA gene. The aims were to identify patterns in diversity and composition of heterotrophic bacterioplankton and to test mechanistic hypotheses for explaining these differences along variations in depth, water mass, phytoplankton biomass, and organic and inorganic nutrients. The overall goal was to clarify the relationship between primary producers and bacterioplankton community structure in the Southern Ocean. Results suggested that both epipelagic and mesopelagic bacterioplankton communities were structured by phytoplankton blooming in the euphotic zone. As chlorophyll a (chl‐a) increased in surface waters, the abundance of surface bacterioplankton increased, but their diversity decreased. Similarity in bacterioplankton community composition between surface‐water sites increased as the bloom progressed, suggesting that algal blooms may homogenize surface‐water bacterioplankton communities at larger spatial scales. Below the euphotic zone, the opposite relationship was found. Mesopelagic bacterioplankton diversity increased with increasing chl‐a in the overlying surface waters. This shift may be promoted by several factors including local increase in organic and inorganic nutrients from particles sinking out of the euphotic zone, an increase in niche differentiation associated with the particle flux, interactions with deep‐dwelling macrozooplankton, and release from competition with primary producers. Additional multivariate analyses of bacterioplankton community structure and nutrient concentrations revealed distinct depth horizons, with bacterioplankton communities having maximum alpha and beta diversity just below the euphotic zone, while nutrient composition gradually homogenized with increasing depth. Our results provide evidence for bloom‐driven (bottom‐up) control of bacterioplankton community diversity in the coastal Southern Ocean and suggest mechanisms whereby surface processes can shape the diversity of bacterioplankton communities at great depth.

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The influence of light and water mass on bacterial population dynamics in the Amundsen Sea Polynya

Cited by 10 publications

References 67 publications

Assessing the composition of microbial communities in textile wastewater treatment plants in comparison with municipal wastewater treatment plants

Assessing the composition of microbial communities in textile wastewater treatment plants in comparison with municipal wastewater treatment plants

Pelagic microbial heterotrophy in response to a highly productive bloom of Phaeocystis antarctica in the Amundsen Sea Polynya, Antarctica

Summer comes to the Southern Ocean: how phytoplankton shape bacterioplankton communities far into the deep dark sea

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