The Influence of Light on the Rate of Respiration of the Blue‐green Alga Anabaena

Brown, Allan H.; Webster, George C.

doi:10.1002/j.1537-2197.1953.tb06551.x

Cited by 59 publications

(21 citation statements)

References 11 publications

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“…Nevertheless evidence from tracer studies was by no means unilateral. In certain species, cases of extreme photoinhibition or of moderate photostimulation of tracer 02 uptake were observed (4,13). Results from radiocarbon labelling experiments by Calvin and associates (1,5) were interpreted as evidence that the Krebs cycle does not operate in bright light and, from kinetic measurements with tracer C02 (21), it was concluded that C02 evolution was reduced by intense light to about one half its value in darkness.…”

Section: Literature Citedmentioning

confidence: 99%

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Brown

Weis

1959

Plant Physiol.

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and c1 would be an underestimate of p, at least during the early stages of equilibration lag. If the vessels were paired for oxygen (1) then a = a' and cl = pl { a K,/Kl -k2/k1 } If, however, there was a big enough burst of CO2 during this period then c1 could exceed 3,k. That is there could be an apparent burst of oxygen.Sufficient has been said to show that estimates of rates of gas production from manometer readings over a relatively short period, subsequent to a change of conditions which result in a change in rate, can be misleading. LITERATURE CITED

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Section: Literature Citedmentioning

confidence: 99%

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Brown

Weis

1959

Plant Physiol.

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“…In one of their experiments, it is apparent that the fastest ratesof 02 uptake occurred whenCO2 fixation (as monitored by 1602 evolution) was the lowest (12 (19). It was postulated that the rapid photoreduction of 02 after a period of darkness served as a priming reaction for the photosynthetic apparatus (19 Unlike previous work with cyanobacteria (3,8,9,12), recent work by Sueltemeyer et al (23,24) with Chlamydomonas has demonstrated an increased rate of 02 uptake with increased DIC concentration. It was concluded that the increased rate of 02 uptake was due to enhanced photoreduction in a Mehler-type reaction, and that extra ATP needed for increased rates of DIC uptake was synthesized as a result (23,24).…”

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“…It was soon found, however, that, when the 02 concentration was higher than that previously used (0.5% in the gas phase) and the PFR was increased, these cells (3) and those of Anacystis and Scenedesmus (8,9) usually did show a light stimulated 02 uptake. The DIC concentration was not controlled in these early experiments, but it must have been capable of supportingCO2 fixation since net 02 evolution was observed (3,8,9). Later, Lex et a l .…”

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confidence: 95%

Active Transport of Inorganic Carbon Increases the Rate of O₂ Photoreduction by the Cyanobacterium Synechococcus UTEX 625

Miller¹,

Espie²,

Canvin³

1988

Plant Physiol.

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Chlorophyll a fluorescence ofSynechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO2 rfxation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of QA, the primary acceptor of photosystem II. Mass spectrometry revealed that transport of inorganic carbon increased the rate of 02 photoreduction. Hence, 02 could serve as an electron acceptor to allow oxidation of QA even in the absence of CO2 fixation.The active transport of HC03-or CO2 causes quenching of Chl a fluorescence in Synechococcus UTEX 625 even when CO2 fixation is inhibited by iodoacetamide (14, 16). Quenching does not occur in the presence of DCMU which suggests that the quenching is due to oxidation of the acceptor QA of PSII (14). In the presence ofiodoacetamide, CO2 reduction cannot be a means for oxidation of QA (14, 16), but it is well established that 02 can also be reduced by noncycic electron transport (1,4,6 In this communication, we present evidence that an increase in qQ is the major reason for the drop in Chl a fluorescence yield resulting from DIC transport. We also show that DIC transport increases the rate of 02 photoreduction. MATERIALS AND METHODSGrowth and Preparation of Cells. Synechococcus UTEX 625 was grown with air bubbling as previously described (5). Cells were washed and resuspended in 25.0 mm BTP/23.5 mM HCI buffer, pH 8.0, which contained low levels (about 15 gM) of DIC (13).Fluorimetry. The fluorescence yield ofChl was monitored with the modulation fluorometer described by Schreiber et al. (21) (PAM-101, H. Walz, D-8521, Effeltrich, FRG). Actinic light to drive DIC uptake and CO2 fixation was from a tungsten-halogen projector lamp with PFR varied with neutral density filters. Fluorescence yield was monitored with a weak (about 2 ME. m2. s_') pulse modulated beam (frequency, I05 s-) and the oxidation state of QA was measured at 20 s intervals with a high intensity white-light flash (1600 uE.m-2.s' for 500 ms). The PAM-101 amplifier system only monitors the fluorescence emission elicited by the modulated light beam, therefore, during the 500 ms saturating flash of actinic light, only changes in fluorescence emission due to changes in the oxidation state ofQA are recorded (21). Fluorescence yield was monitored at the same time as 02 and DIC fluxes were being measured by MS. The Fm was determined in the presence of 20 uM DCMU (14). The Fo was estimated with the modulated light beam at a very low PFR and a frequency of 1.6 x 10-3 s-'. For cyanobacteria, we defined F, which is essentially the same as (F,)m of Schreiber et al. (21), as .Mass Spectrometry. Dissolved 1602, 1802, '2C02, and "3CO2 (m/z = 32, 36, 44, and 45) in the cell suspension (details in figure legends) were sequentially measured at 7-to 15-s intervals using a magnetic sector mass spectrometer (VG Gas Analysis, Middlewich, England, model MM 14-80 SC) equipped with a membrane inlet system (15). Data were stored on magnetic disc fo...

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“…Suppression of respiratory oxygen uptake by light has been noted for both bluegreen algae (Brown & Webster, 1953, Hoch, Owens & Kok, 1963 and purple bacteria (van Niel, 1941;Fork & Goedheer, 1964). To explain the phenomenon Brown & Webster (1953) suggested that either the photosynthetically produced oxygen was utilized within the organism (an explanation which cannot hold for the bacteria in which oxygen is not a product of photosynthesis), or that light inhibited respiratory electron transfer.…”

Section: Discussionmentioning

confidence: 99%

“…To explain the phenomenon Brown & Webster (1953) suggested that either the photosynthetically produced oxygen was utilized within the organism (an explanation which cannot hold for the bacteria in which oxygen is not a product of photosynthesis), or that light inhibited respiratory electron transfer. Assuming that PICP is acting in both presence and absence of light merely as an electron acceptor, then our results support the latter suggestion.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Phenolindo-2,6-dichlorophenol in Dark and Light by the Blue-Green Alga, Anabaena variabilis

Carr¹,

Hallaway²

1965

Journal of General Microbiology

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SUMMARYThe reduction of phenolindo-2,6-dichlorophenol (PICP) by the biuegreen alga Anabaena variabilis was examined in the dark and in the light. The two reductions differed in order with respect to PICP, in pH optimum, response to cyanide and iodoacetate, and in sensitivity to starvation and lysozyme treatment of the organism. The reduction in the dark was suppressed at light intensities of 300 ft.-candles. These results are discussed in relation to work on the photo-inhibition of respiratory processes in this and other micro-organisms.

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The Influence of Light on the Rate of Respiration of the Blue‐green Alga Anabaena

Cited by 59 publications

References 11 publications

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Active Transport of Inorganic Carbon Increases the Rate of O₂ Photoreduction by the Cyanobacterium Synechococcus UTEX 625

Reduction of Phenolindo-2,6-dichlorophenol in Dark and Light by the Blue-Green Alga, Anabaena variabilis

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The Influence of Light on the Rate of Respiration of the Blue‐green Alga Anabaena

Cited by 59 publications

References 11 publications

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Active Transport of Inorganic Carbon Increases the Rate of O2 Photoreduction by the Cyanobacterium Synechococcus UTEX 625

Reduction of Phenolindo-2,6-dichlorophenol in Dark and Light by the Blue-Green Alga, Anabaena variabilis

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Active Transport of Inorganic Carbon Increases the Rate of O₂ Photoreduction by the Cyanobacterium Synechococcus UTEX 625