So, the use of N-acetyl-D-glucosamine as an additional agent of carbohydrate nature in inoculants with soybean nodule bacteria and soil diazotrophs of Azotobacter genus led to a more complete realization of the symbiotic and productive potential of phyto-bacterial symbiosis and association when compared to using a diazotrophs only.Our results indicated the possibility of practical use of acetylated glucose-containing aminosaccharide in the creation of complex inoculants based on nitrogen-fixing bacteria.