The influence of locally applied granulocyte‐colony stimulating factor on osteoporotic bone

Liu, Xi Ling; Rao, Nianjing; Huo, Lei; Hu, Xiang‐Qun; Lu, Wenjia; Zheng, Li Wu

doi:10.1111/clr.12839

Cited by 1 publication

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Improved bone formation was also seen in several studies which have locally applied G-CSF to the defect site (Ishida et al, 2010 ; Minagawa et al, 2014 ; Liu et al, 2017a , b ). In particular, Ishida et al demonstrated an increased capillary density in the bone defect, detected after local G-CSF administration but also in a control group receiving sc G-CSF.…”

Section: Discussionmentioning

confidence: 71%

“…In the context of bone regeneration, Ishida et al ( 2010 ) demonstrated that treatment of a segmental bone defect in the rabbit ulna with a G-CSF loaded gelatin hydrogel resulted in accelerated bone formation. In line with this, it was shown that local delivery of G-CSF to osteoporotic bone fractures (Liu et al, 2017b ) and a rat calvarial defect (Minagawa et al, 2014 ) promoted new bone formation in both models. Assuming that an enhanced accumulation of stem cells in the circulation would facilitate their homing capacity, Marmotti et al ( 2013 ) investigated the effect of preoperative administration of G-CSF in patients undergoing high tibial valgus osteotomy with bone graft substitution.…”

Section: Introductionmentioning

confidence: 74%

See 1 more Smart Citation

Five Days Granulocyte Colony-Stimulating Factor Treatment Increases Bone Formation and Reduces Gap Size of a Rat Segmental Bone Defect: A Pilot Study

Herrmann¹,

Zeiter²,

Eberli³

et al. 2018

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Bone is an organ with high natural regenerative capacity and most fractures heal spontaneously when appropriate fracture fixation is provided. However, additional treatment is required for patients with large segmental defects exceeding the endogenous healing potential and for patients suffering from fracture non-unions. These cases are often associated with insufficient vascularization. Transplantation of CD34+ endothelial progenitor cells (EPCs) has been successfully applied to promote neovascularization of bone defects, however including extensive ex vivo manipulation of cells. Here, we hypothesized, that treatment with granulocyte colony-stimulating factor (G-CSF) may improve bone healing by mobilization of CD34+ progenitor cells into the circulation, which in turn may facilitate vascularization at the defect site. In this pilot study, we aimed to characterize the different cell populations mobilized by G-CSF and investigate the influence of cell mobilization on the healing of a critical size femoral defect in rats. Cell mobilization was investigated by flow cytometry at different time points after five consecutive daily G-CSF injections. In a pilot study, bone healing of a 4.5-mm critical femoral defect in F344 rats was compared between a saline-treated control group and a G-CSF treatment group. In vivo microcomputed tomography and histology were applied to compare bone formation in both treatment groups. Our data revealed that leukocyte counts show a peak increase at the first day after the last G-CSF injection. In addition, we found that CD34+ progenitor cells, including EPCs, were significantly enriched at day 1, and further increased at day 5 and day 11. Upregulation of monocytes, granulocytes and macrophages peaked at day 1. G-CSF treatment significantly increased bone volume and bone density in the defect, which was confirmed by histology. Our data show that different cell populations are mobilized by G-CSF treatment in cell specific patterns. Although in this pilot study no bridging of the critical defect was observed, significantly improved bone formation by G-CSF treatment was clearly shown.

show abstract

Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 74%

Five Days Granulocyte Colony-Stimulating Factor Treatment Increases Bone Formation and Reduces Gap Size of a Rat Segmental Bone Defect: A Pilot Study

Herrmann¹,

Zeiter²,

Eberli³

et al. 2018

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

The influence of locally applied granulocyte‐colony stimulating factor on osteoporotic bone

Abstract: The results indicated that in osteoporotic bone, G-CSF may advance bone healing in cranial bone where spontaneous bone formation was insufficient.

Cited by 1 publication

References 25 publications

Five Days Granulocyte Colony-Stimulating Factor Treatment Increases Bone Formation and Reduces Gap Size of a Rat Segmental Bone Defect: A Pilot Study

Five Days Granulocyte Colony-Stimulating Factor Treatment Increases Bone Formation and Reduces Gap Size of a Rat Segmental Bone Defect: A Pilot Study

Contact Info

Product

Resources

About