The influence of mild dysplasia at the surgical margin on the prognosis of oral squamous cell carcinoma

Pu, Yaoyu; Wang, Yishuang; Huang, Xiaofeng; Chen, S.; Wang, Z.; Sun, Guobin; Tang, Enyi; Zhao, Shaodong; Ni, Yanhong; Hu, Qingang

doi:10.1016/j.ijom.2016.08.019

Cited by 17 publications

(12 citation statements)

References 18 publications

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“…Twelve hours after ICG injection, tumor resection under NIF imaging showed fluorescence in tumor regions in all 48 mice. The average tumor volume was 9.51±9.74 mm 3 , with a minimum volume of less than 0.5 mm 3 . The average fluorescence intensities of the tumor and normal tissues were 123.2±5.6 and 41.2±7.8 AUs,…”

Section: Icg Localizes To Tumor Cells In Vitro and Tumors In Micementioning

confidence: 97%

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Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Pan

Han

et al. 2020

Preprint

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Background Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperative for OSCC. Methods Cytological and animal experiments were first performed to confirm the feasibility of monitoring surgical margins with NIF imaging and spectroscopy. Then, 20 patients with OSCC were included in the clinical trials. At 6–8 hours after 0.75 mg/kg indocyanine green (ICG) injection, all patients underwent surgery with NIF imaging. During the surgery, both NIF images and quantified fluorescence intensity were acquired to monitor the surgical margins. Results In cytological and animal experiments, the results showed it is feasible to monitor surgical margins with NIF imaging and spectroscopy. Fluorescence was detected in primary tumors in all patients. The fluorescence intensities of the tumor, peritumoral, and normal tissues were 398.863±151.47, 278.52±84.89, and 274.5±100.93 arbitrary units (AUs), respectively (P<0.05). The SBR of tumor to peritumoral tissue and normal tissues was computed to be 1.45±0.36 and 1.56±0.41 respectively. After primary tumor excision, the wounds showed abnormal fluorescence in four patients (4/20), and residual cancer cells were confirmed by pathological examination in two patients (2/20). Conclusion These findings confirmed the complementary value of NIF imaging during radical tumor resection of OSCC.

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Section: Icg Localizes To Tumor Cells In Vitro and Tumors In Micementioning

confidence: 97%

“…The surgical margin is the only accessible 3 approach to detect remaining cancer cells after excision, which is vital to the prognoses of patients with OSCC 2 . Positive surgical margins are associated with significantly reduced survival rates 3 .…”

Section: Introductionmentioning

confidence: 99%

Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Pan

Han

et al. 2020

Preprint

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“…Margin status and invasion pattern in tumor nest have been proved to be factors of prognostic significance for OTSCC patients . As an attempt to develop a method to comprehensively study the margin status and the invasion pattern, we made serial whole‐mount sections and performed 3D reconstruction.…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional reconstruction with serial whole‐mount sections of oral tongue squamous cell carcinoma: A preliminary study

Wang

Chen

et al. 2017

J Oral Pathology Medicine

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Objectives: Margin status and invasion pattern are prognostic factors for oral tongue squamous cell carcinoma (OTSCC). Current methods to identify these factors are limited to 2D observation; it is necessary to explore 3D reconstruction with whole-mount sample to improve the accuracy of analysis. This study aimed to study the tissue preparation, section generation, and 3D reconstruction with whole-mount OTSCC specimen.Study design: Two OTSCC samples were retrieved from Nanjing Stomatological Hospital, Medical School of Nanjing University. One sample was sliced into 3 equalsized pieces and subjected to different processing schedules to determine the best method. The second sample was processed accordingly. Serial whole-mount sections of the second sample were generated, stained with HE/anticytokine antibody in intersection manner, and scanned into digital images. Digital images were aligned and reconstructed into 3D images with Hetero Genius Medical Image Manager 3D Pathology Add-On [HGMIM3D].Results: Successful serial whole-mount sections of comparable quality to traditional sections were generated. Three-dimensional images with serial whole-mount sections were successfully generated.Conclusions: Whole-mount histopathological 3D reconstruction of OTSCC was successfully generated, providing a solid foundation for comprehensive margin and invasion analysis. Although future study and improvement were needed, wholemount histopathological 3D reconstruction proved to be a promising method in OTSCC study. K E Y W O R D Sinvasion, margin status, oral tongue squamous cell carcinoma, serial whole-mount sections, three-dimensional reconstruction

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Section: Icg Localizes To Tumor Cells In Vitro and Tumors In Micementioning

confidence: 95%

“…The surgical margin is the only accessible approach to detect remaining cancer cells after excision, which is vital to the prognoses of patients with OSCC 2 . Positive surgical margins are associated with significantly reduced survival rates 3 . Currently, identification of the tumor border is typically dependent on intraoperative visual and tactile examination.…”

Section: Introductionmentioning

confidence: 99%

Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Pan

Han

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperatively for OSCC. Methods Cytological and animal experiments were first performed to confirm the feasibility of monitoring surgical margins with NIF imaging and spectroscopy. Then, 20 patients with OSCC were included in the clinical trials. At 6–8 hours after 0.75 mg/kg indocyanine green (ICG) injection, all patients underwent surgery with NIF imaging. During the surgery, both NIF images and quantified fluorescence intensity were acquired to monitor the surgical margins. Results In cytological and animal experiments, the results showed it was feasible to monitor surgical margins with NIF imaging and spectroscopy. Fluorescence was detected in primary tumors in all patients. The fluorescence intensities of the tumor, peritumoral, and normal tissues were 398.863±151.47, 278.52±84.89, and 274.5±100.93 arbitrary units (AUs), respectively (P<0.05). The SBR of tumor to peritumoral tissue and normal tissues was computed to be 1.45±0.36 and 1.56±0.41 respectively. After primary tumor excision, the wounds showed abnormal fluorescence in four patients (4/20), and residual cancer cells were confirmed by pathological examination in two patients (2/20). Conclusion These findings confirmed the complementary value of NIF imaging during radical tumor resection of OSCC. Before tumor resection, we could utilize the fluorescence margin produced by ICG NIF imaging to determine the surgical margin. Moreover, after tumor blocks were removed, the status of surgical margin could also be evaluated rapidly by ICG NIF imaging of tumor bed and in vitro specimens.

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The influence of mild dysplasia at the surgical margin on the prognosis of oral squamous cell carcinoma

Cited by 17 publications

References 18 publications

Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

Three‐dimensional reconstruction with serial whole‐mount sections of oral tongue squamous cell carcinoma: A preliminary study

Real-time surveillance of surgical margins via ICG-based near-infrared fluorescence imaging in patients with OSCC

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