The influence of monogalactosyldiacylglycerols from different marine macrophytes on immunogenicity and conformation of protein antigen of tubular immunostimulating complex

Sanina, N. M.; Kostetsky, E. Ya.; Shnyrov, Valery L.; Tsybulsky, A. V.; Novikova, O. D.; Portniagina, O. Yu.; Vorobieva, Natalia; Mazeika, Andrey; Bogdanov, Mikhail

doi:10.1016/j.biochi.2012.01.009

Cited by 27 publications

(40 citation statements)

References 31 publications

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“…Inclusion of HA0 S in TI-complexes resulted in the 2-fold increase in the content of anti-HA0 S antibodies. Unlike previous results on the porin of Yersinia pseudotuberculosis [Sanina et al, 2012], variation of MGDG constituent of TI-complexes did not influence the level of anti-HA0 S antibodies. However, DSC and especially spectroscopic studies revealed remarkable changes of the HA0 S conformation depending on the microviscosity of MGDGs.…”

Section: Discussioncontrasting

confidence: 55%

“…The absence of the similar effect of MGDGs on immune response against HA0 s of influenza A virus and porin of Y. pseudotuberculosis [Sanina et al, 2012] suggests that the immune response is dependent not only on the components of the TI-complex and their ratio [Kostetsky et al, 2011] but the particular structure of the antigen in the content of this promising nanoparticulate adjuvant delivery system.…”

Section: Discussionmentioning

confidence: 96%

“…However, only nonlamellar glycoglycerolipid MGDG was shown to form superstructure of TI-complexes. It performs the role of a lipid matrix for incorporated protein antigen [Sanina et al, 2004[Sanina et al, , 2012. Physical state of MGDG depends on its fatty acid composition, which in turn varies in dependence of taxonomic position of marine macrophyte.…”

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confidence: 99%

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On the Possibility of Lipid-Induced Regulation of Conformation and Immunogenicity of Influenza A Virus H1/N1 Hemagglutinin as Antigen of TI-Complexes

Vorobieva

Sanina²,

Vorontsov

et al. 2014

Microb Physiol

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The tubular immunostimulating complex (TI-complex) consisting of cucumarioside A₂-2, cholesterol and monogalactosyldiacylglycerol (MGDG) from marine macrophytes is the perspective antigen delivery system for subunit vaccines. MGDG is a lipid matrix for the protein antigen incorporated in the TI-complex. The aim of the present work was to study the influence of MGDGs from different macrophytes on conformation and immunogenicity of the secreted recombinant uncleaved hemagglutinin monomer (HA0_S) of influenza A virus H1/N1. Differential scanning calorimetry, fluorescence spectroscopy and circular dichroism showed a dependence of the conformational changes of HA0_S on the microviscosity of MGDG. The most viscous MGDG from Zostera marina induced the strongest rearrangements in protein conformation. Immunization of mice with HA0_S within TI-complexes comprising different MGDGs resulted in an approximately 2-fold increase of the levels of anti-HA0_S antibodies and granulocyte-macrophage colony-stimulating factor (GM-CSF) compared with those induced by HA0_S alone. TI-complexes based on MGDG from Z. marina stimulated the maximal production of GM-CSF. However, humoral immune response (anti-HA0_S antibodies), unlike cell-mediated immune response (GM-CSF), did not depend on the physicochemical properties of MGDGs. It is assumed that this is due to the different localization and conformational lipid sensitivity of the HA0_S regions, which are responsible for these types of immune responses.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 96%

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On the Possibility of Lipid-Induced Regulation of Conformation and Immunogenicity of Influenza A Virus H1/N1 Hemagglutinin as Antigen of TI-Complexes

Vorobieva

Sanina²,

Vorontsov

et al. 2014

Microb Physiol

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“…Calorimetric experiments with porin were performed on a Scal-1 differential scanning microcalorimeter (Scal Co., Ltd., Pushchino, Russia), as described previously [6]. Before measurement, the sample and reference solutions were thoroughly degassed and carefully loaded into the cells to avoid bubble formation.…”

Section: Calorimetrymentioning

confidence: 99%

“…However, our study of the properties of the hybrid system, comprising glycolipid monogalactosyldiacylglycerol from different marine macrophytes and YOmpF led to the conclusion that physicochemical properties of lipid surroundings are very important for conformation and immunogenicity of this protein [6].…”

Section: Introductionmentioning

confidence: 99%

Effect of phenol‐induced changes in lipid composition on conformation of OmpF‐like porin of Yersinia pseudotuberculosis

et al. 2013

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a b s t r a c tThe present work aimed to compare the effects of different lysophosphatidylethanolamine (LPE) content in lipids derived from Yersinia pseudotuberculosis cells exposed and not exposed to phenol on the conformation of OmpF-like porin of these bacteria. Differential scanning calorimetry and intrinsic protein fluorescence showed that the 2.5-fold increase of LPE content and the corresponding increase in the phase transition temperature of bacterial lipids were accompanied by enhanced protein thermostability. Integral conformational rearrangement of protein was supported by drastic changes in the microenvironment of the tryptophan residues, likely resulting in a convergence of monomers in trimeric porin and exposure of outer tryptophan residues to the water environment. These conformational changes may impede the porin channel permeability under stress conditions in bacteria.

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Glycolipids from Sargassum filipendula, a Natural Alternative for Overcoming ABC Transporter‐Mediated MDR in Cancer

Muñoz‐Losada,

Da Costa,

Muñoz‐Castiblanco

et al. 2023

Chemistry & Biodiversity

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Chemotherapy is a widely used strategy to treat cancer, a disease that causes millions of deaths each year. However, its efficacy is reduced by the overexpression of ABC transporters, which are proteins that expel the drugs used in chemotherapy and involved in the multidrug resistance (MDR). Glycolipids have been identified as potential inhibitors of ABC transporters. Algae of the genus Sargassum contain high levels of glycolipids, being a promising therapeutic alternative against the MDR phenotype. Sargassum filipendula glycolipids were obtained by exhaustive maceration with chloroform/methanol, purified by column and thin layer chromatography, and then characterized by FTIR, NMR, and LC‐MS. Cell viability by PI labeling and inhibition of ABC transporters were analyzed by flow cytometry. Assessment of resistance reversal was determined by MTT assay. Ten sulfoquinovosylglycerol‐type compounds were found, and six of them are reported for the first time. Moiety 4 (GL‐4) showed strong and moderate inhibitory activity against ABCC1 and ABCB1 transporters, respectively. Treatment of GL‐4 in combination with the antineoplastic drug vincristine sensitized Lucena‐1 cell model to drug and reversed the MDR phenotype. This is the first report of glycolipids isolated from S. filipendula capable of inhibiting ABC transporters and thus overcoming acquired drug resistance.

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The influence of monogalactosyldiacylglycerols from different marine macrophytes on immunogenicity and conformation of protein antigen of tubular immunostimulating complex

Cited by 27 publications

References 31 publications

On the Possibility of Lipid-Induced Regulation of Conformation and Immunogenicity of Influenza A Virus H1/N1 Hemagglutinin as Antigen of TI-Complexes

On the Possibility of Lipid-Induced Regulation of Conformation and Immunogenicity of Influenza A Virus H1/N1 Hemagglutinin as Antigen of TI-Complexes

Effect of phenol‐induced changes in lipid composition on conformation of OmpF‐like porin of Yersinia pseudotuberculosis

Glycolipids from Sargassum filipendula, a Natural Alternative for Overcoming ABC Transporter‐Mediated MDR in Cancer

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