The influence of mRNA secondary structure on expression of endoglucanase inBacillus subtilis

Simultaneous overproduction of extracellular endoglucanase and intracellular ?-glucosidase by genetically engineered Bacillus subtilis

“…The plasmid pBJ27UA88E (Kim and Pack, 1993), which contains an endoglucanase structural gene fused to a chemically synthesized SD sequence under the control of the strong engineered promoter BJ27UA88 (Lee et al, 1994) was partially digested by HindIII to open each end of endoglucanase gene. To construct operon systems, the plasmid pB which contains a B-glucosidase gene fused to the SD sequence of gene 10 of phage T7(accompanying paper) was digested by HindlII.…”

“…The studies were further extended to biochemical and microbiological studies using purified endoglucanase from E. coli, B. subtilis and B. megaterium to characterize enzymatic properties as well as to select suitable bacterial host strains for high enzyme production (Lee and Pack, 1987;Kim and Pack, 1988;Lee et al, 1988). One of them, the strong promoter !115 BJ27UA88 (Lee et al, 1994), which is a modified form of parental promoter BJ27, was successfully used for overproduction of endoglucanase or B-glucosidase, separately in B. To express these genes in B. subtilis during vegetative growth, many strong promoter fragments in B. subtilis chromosome were cloned and characterize~!.…”