The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice

Evaluation of the Effect of Psyllium on the Viability of Lactobacillus Acidophilus in Alginate-Polyl Lysine Beads

“…The obtained high EE indicated that there was no considerable loss of viability in the process of beads preparation, confirming the gentle propriety of the applied method. 20 Furthermore, there were no significant differences (P>0.05) regarding the EE between different formulations. The rough and approximately porous surface characteristics of alginate beads shown in SEM pictures is in good agreement with the other studies which can be explained by the egg-box structure of calcium alginate beads.…”

Microencapsulation of Probiotics by Calcium Alginate and Gelatin and Evaluation of its Survival in Simulated Human Gastro-Intestinal Condition

“…conditions to which probiotics can be exposed (Mokarram et al, 2009). Several studies have been carried out investigating the protective role of this technique.…”

“…Lyophilized cells were inoculated in MRS broth (de Man-RogasaSharpe) for 24 h under aerobic conditions at 37°C.Biomasses were then harvested by centrifuging at 5000 rpm for 10 min at 4 °C. The cultures were then washed twice by sterile saline solution (0.9%) and used in the microencapsulation process (Mokarram et al, 2009).…”

“…The capsules containing probiotic bacteria were released by citrate buffer (pH= 6.0, 1 %) reported by Mokarram et al, in 2009. One gram of capsules was transferred to 9 mL buffer.…”

Comparative survival of commercial probiotic formulations: tests in biorelevant gastric fluids and real-time measurements using microcalorimetry

“…Gastric tolerance assays for potential new probiotic formulations (Chandramouli et al 2004;Cook et al 2011;Cui et al 2000;Ding et al 2009;Mokarram et al 2009) and commercial probiotic products (Sahadeva et al 2011) or organisms (Jensen et al 2012) routinely use either buffer or saline solutions adjusted to pH 1.2 to 4, or growth media, adjusted to these same pH's, to simulate the gastric juice. These simulated fluids often lack the complexity of real gastric fluid and gastric volumes are often not accounted for or ignored.…”