This is an exploratory analysis combining artificial intelligence algorithms, fishery acoustics technology, and a variety of abiotic factors in low-latitude coastal waters. This approach can be used to analyze the sensitivity level between the acoustic density of fishery resources and various abiotic factors in the surface mixed layer (the water layer above the constant thermocline) and the bottom cold water layer (the water layer below the constant thermocline). The fishery acoustic technology is used to obtain the acoustic density of fishery resources in each water layer, which is characterized by Nautical Area Scattering Coefficient values (NASC), and the artificial intelligence algorithm is used to rank the sensitivity of various abiotic factors and NASC values of two water layers, and the grades are classified according to the cumulative contribution percentage. We found that stratified or multidimensional analysis of the sensitivity of abiotic factors is necessary. One factor could have different levels of sensitivity in different water layers, such as temperature, nitrite, water depth, and salinity. Besides, eXtreme Gradient Boosting and random forests models performed better than the linear regression model, with 0.2 to 0.4 greater R 2 value. The performance of the models had smaller fluctuations with a larger sample size. The thermocline in the low latitude sea area is permanent, the upper layer of the thermocline is a surface mixed layer, and the lower layer of the thermocline is the bottom cold water layer. The distribution of fishery organisms and their driving factors in the perfusing water layers (especially the surface mixed layer and the bottom cold water layer) deserves further investigation. The offshore of the northern South China Sea is a typical representative of low-latitude coastal waters, and it is also an important traditional fishery production operation in China, owing to its good climate that offers a conducive habitat for the marine life, a spawning site, and a place for fattening and farming of fish. However, fishery resources have increasingly become small and of reduced quality 1,2. Resource density, the single yield of fishing vessels, and catch quality are declining. The catch rates