The Influence of Phenylbutazone on Collagen Metabolism <i>in vivo</i>

Abstract

The effect of phenylbutazone in daily doses of 100 mg/kg on collagen metabolism in rats was studied after an injection of ¹⁴C-proline, by determining specificand total ¹⁴C-hydroxyproline activity in the skin collagen fractions and urine. It was found that a 21-day administration of phenylbutazone influenced the earlyphases of collagen metabolism by inhibiting collagen biosynthesis. The later phases of collagen turnover (maturation) were not affected by phenylbutazone treatment. In the given dose, phenylbutazone did not stimulate the catabolism of either newly-formed, soluble collagen, or mature, insoluble collagen.

Systemic administration of glycosaminoglycan polysulphate (Arteparon) provides partial protection of articular cartilage from damage produced by meniscectomy in the canine

“…Furthermore, both total and partial meniscectomy in a variety of laboratory animals have been known for many years to initiate degenerative changes in articular cartilage (14,23,24,48); indeed. some workers have used meniscectomv in administered with some success to suppress the inflammation associated with meniscectomy (57), this and similar nonsteroidal anti-inflammatory agents would have little or no beneficial effect on articular cartilage, and in some instances may exacerbate (62) the problem by suppressing the biosynthesis of proteoglycans (PGs) (5334) and collagen (74) in these tissues.…”

The Influence of Chloroquine Upon the Metabolism of Collagen in Rat

“…In this con nexion are remarkable the findings of Morches et al demonstrat ing an increase of serum cathepsin activity in rats after chloro quine treatment [9], Otherwise chloroquine causes an inhibition of most enzymatic activities, but lor the inhibition of skin col lagénase in vitro an extremely high concentration (up to 10 him) [3] is required. The enhanced break-down of the newly formed collagen could explain our earlier report on the favourable in fluence of chloroquine on lathyritic collagen lesions [15]. Treat ment with chloroquine resulted in lathyritic rats in a reduction of the abnormal increased levels of soluble skin collagens.…”

“…There are only a few reports dealing with the influence of chloroquine upon the metabolism of collagen and consist of studies without the use of "C-proline [6,14,15]. Our experiment was aimed at the determination of the effects of chloroquine upon the meta bolism of collagen using "C-proline.…”

Influence of Nonsteroidal Antirheumatic Drugs on Collagen Metabolism in Rats with Adjuvant-Induced Arthritis

“…The final effect o f phenylbutazone on the metabolism o f collagen in healthy animals consists in an inhibition of the early phases of collagen biosynthesis (9). In the group of animals with adjuvant-induced arthritis treated with phenyl butazone, the collagen synthesis is likewise moderately reduced in comparison with the group of untreated arthritis animals.…”