The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats

Wróbel, Andrzej; Juszczak, Kajetan; Adamowicz, Jan; Drewa, Tomasz; Dudka, Jarosław

doi:10.1016/j.biopha.2020.110861

Cited by 7 publications

(8 citation statements)

References 45 publications

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“…This leads to the conclusion that Urox ® is potent in resolving OAB symptoms in our model. The normalization of the storage phase parameters may be due to the desensitization of the afferent fibers reaching the urinary bladder, as described previously ( Wróbel et al, 2020 ). What is worth emphasizing is that no influence on MVP and PVR were observed, which suggests that Urox ® does not compromise normal voiding.…”

Section: Discussionmentioning

confidence: 99%

“…The mean values from all animals in each condition were averaged to create pooled data for each condition. The cystometric parameters were defined previously ( Wróbel et al, 2015 ; Wróbel et al, 2020 ). All procedures were performed by a person blinded to the treatments.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, we had confirmed that detrusor overactivity was induced by retinyl acetate (RA) in rats ( Wróbel et al, 2015 ; Wróbel et al, 2020 ) and described that Potentilla chinensis extract (PCE) has the potential to reverse RA–induced detrusor overactivity (DO) ( Wróbel et al, 2020 ). The aim of the current study was to investigate whether phytomedicine extracts from Lindera aggregata root, Equisetum arvense stem, and Crateva nurvala stem bark (Urox ® ) would reverse RA-induced changes in several cystometric and biochemical parameters characteristic of bladder overactivity and to thus check if this herbal supplement could be a reasonable strategy, as a future pharmacological treatment for patients with OAB.…”

Section: Introductionmentioning

confidence: 97%

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New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

Zapała

Juszczak²,

Adamczyk³

et al. 2022

Front. Mol. Biosci.

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The aim of the current study was to determine if phytomedicine (Urox®) would reverse retinyl acetate (RA)–induced changes characteristic of bladder overactivity. There were 60 rats divided into the following 4 groups: I—control, II—received RA to induce detrusor overactivity (DO), III—received Urox (840 mg daily for 14 days), and IV—received combination of RA and Urox®. The cystometry was performed 2 days after the last dose of Urox®. Next, urothelium thickness and biochemical parameter measurements were performed. In group IV, a decrease in basal pressure and detrusor overactivity index was noted when compared to group II. Furthermore, in group IV the following parameters were increased: threshold pressure, voided volume, intercontraction interval, and bladder compliance in comparison with group II. There were significant elevations in c-Fos expression in the neuronal voiding centers in group II, while the expression of c-Fos in group IV was normalized. No significant changes in the values of the analyzed biomarkers in group III were found, while in group II, an elevation in BDNF, NGF, CGRP, ATP, Rho kinase, malondialdehyde, 3-nitrotyrosine, TRPV1, OCT-3, and VAChT and then a decrease in E-cadherin and Z01 were found. A successful restoration of all the abovementioned biomarkers’ levels was observed in group IV. Phytomedicine extracts (Urox®) were found to be potent in reversing RA-induced changes in several cystometric and biochemical parameters that are determinants of overactive bladder (OAB). The actions of Urox® were proved to be dependent on several factors, such as growth factors and several OAB biomarkers but not pro-inflammatory cytokines.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

Zapała

Juszczak²,

Adamczyk³

et al. 2022

Front. Mol. Biosci.

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“…A voiding contraction was identified as a large increase in bladder pressure accompanied by the release of fluid from the urethra, (10) area under curve (AUC): defined the area under the cystometrogram trace reflecting the motility activity of the detrusor—we used total AUC method which determines area under the curve 13 , (11) detrusor index (DI, cmH 2 O/ml) (in group I and III) and detrusor overactivity index (DOI, cmH 2 O/ml) (in group II and IV): depicted as a quotient of the sum of amplitudes of all detrusor contractions during the filling phase (all NVCs and micturition voiding pressure) and functional bladder capacity. During calculation of DOI, the baseline bladder pressure (basal pressure at the beginning of the detrusor contraction) was subtracted from the height (absolute value of the contraction—from 0 cmH 2 O) of each detrusor contractions (each NVCs and micturition voiding pressure) 9 , 15 .…”

Section: Methodsmentioning

confidence: 99%

“…Potentilla chinensis extract (PCE) contains polyphenols which present antioxidative effects. These properties of PCE appear to be important in terms of preventing of oxidative stress-dependent diseases development 8 , 9 . Previous studies described several bioactive compounds which were extracted from Potentilla chinensis .…”

Section: Introductionmentioning

confidence: 99%

Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model

Juszczak

Adamowicz

Zapała

et al. 2022

Sci Rep

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Cyclophosphamide (CYP) damages all mucosal defence lines and induces hemorrhagic cystitis (HC) leading to detrusor overactivity. Patients who undergo combined chemio-radiotherapy are at higher risk of HC. Potentilla chinensis extract (PCE) prevent oxidative stress-dependent diseases. Thus, the aim of the study was to investigate the effect of PCE on urinary bladder function in CYP-induced HC in preclinical study. 60 rats were divided into 4 groups, as follows: I—control, II—rats with CYP-induced HC, III—rats received PCE in dose of 500 mg/kg, and IV—rats with CYP-induced HC which received PCE in dose of 500 mg/kg. PCE or vehicle were administered orally for 14 days. The cystometry was performed 3 days after the last dose of the PCE. Next, urothelium thickness and oedema measurement and biochemical analyses were performed. Cyclophosphamide induced hemorrhagic cystitis. PCE had no influence on the urinary bladder function and micturition cycles in normal rats. PCE diminished the severity of CYP-induced hemorrhagic cystitis. In the urothelium the cyclophosphamide induced the elevation of CGRP, TNF-α, IL-6, IL-1β, OTC3, NIT, and MAL. Also, the level of T-H protein, HB-EGF, and ZO1 was decreased. Moreover, the level of ROCK1 and VAChT in detrusor muscle increased. cyclophosphamide caused an increased concentration of BDNF and NGF in the urine. In turn, PCE in cyclophosphamide-induced hemorrhagic cystitis caused a reversal of the described biochemical changes within urothelium, detrusor muscle and urine. PCE attenuates detrusor overactivity. In conclusion, our results revealed that PCE attenuates detrusor overactivity in case of cyclophosphamide-induced hemorrhagic cystitis. The potential properties of PCE appear to be important in terms of preventing of oxidative stress-dependent dysfunction of urinary bladder. PCE may become a potential supportive treatment in patient to whom cyclophosphamide-based chemotherapy is used.

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The Orexin OX₂ Receptor‐Dependent Pathway Is Implicated in the Development of Overactive Bladder and Depression in Rats Exposed to Corticosterone

Serefko,

Wróbel,

Szopa

et al. 2024

Neurourology and Urodynamics

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AimIn the present study, we wanted to check whether TCS OX2 29 (TCS), a potent selective antagonist of OX2 receptors, would have positive effects in an animal model of detrusor overactivity co‐existed with the depression‐like state in Wistar male rats.MethodsThe forced swim test with the measurement of spontaneous locomotor activity, conscious cystometry, determination of c‐Fos expression in central micturition areas, and a set of biochemical analyses (with the use of urine, hippocampus, bladder urothelium, and detrusor muscle of tested animals) were carried out.ResultsThe outcomes showed that a 7‐day administration of TCS (3 mg/kg/day, subcutaneously) normalizes the cystometric parameters corresponding to overactivity of the detrusor and reverses the pro‐depressive response. Furthermore, the antagonism of OX2 receptors restored the abnormal levels of overactive bladder markers (i.e., ATP, CGRP, OCT3, TRPV1, ROCK1, and VAChT), diminished neuronal overactivity in central micturition areas (i.e., pontine micturition center, ventrolateral periaqueductal gray, and medial preoptic area) as well as restored the altered hippocampal levels of CRF, cytokines (IL‐1β, IL‐6, IL‐10, and TNF‐α), and growth factors (BDNF and NGF) that reflected biochemical disturbances detected in depressed people.ConclusionsIt seems that our findings open new perspectives regarding the implication of the orexin system in the functioning of the urinary bladder and in the pathophysiology of depression.

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The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats

Cited by 7 publications

References 45 publications

New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model

The Orexin OX₂ Receptor‐Dependent Pathway Is Implicated in the Development of Overactive Bladder and Depression in Rats Exposed to Corticosterone

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The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats

Cited by 7 publications

References 45 publications

New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats

Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model

The Orexin OX2 Receptor‐Dependent Pathway Is Implicated in the Development of Overactive Bladder and Depression in Rats Exposed to Corticosterone

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The Orexin OX₂ Receptor‐Dependent Pathway Is Implicated in the Development of Overactive Bladder and Depression in Rats Exposed to Corticosterone