The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain

Alsanie, Walaa F.; Abdelrahman, Sherin; Felimban, Raed I.; Alkhatabi, Heba; Gaber, Ahmed; Alosimi, Ebtisam; Alhomrani, Majid; Habeeballah, Hamza; Hauser, Charlotte; Alamri, Abdulhakeem S.; Althobaiti, Aiysha Nasser; Alsharif, Abdulaziz; Alzahrani, Ahmed S.; Al-Ghamdi, Mohammad S.; Raafat, Bassem M.; Alswat, Khaled; Althobaiti, Yusuf S.; Asiri, Yousif A.

doi:10.3390/ijms24065668

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…The claimed prevalence rates of substance misuse in Islamic nations are influenced by various factors. [ 10 11 12 13 14 ]…”

Section: Discussionmentioning

confidence: 99%

Parents’ Knowledge, Attitude, and Practices toward Methamphetamine Abuse among Youth and its Risk Factors in Saudi Arabia

Ibrahiem,

Al Dawsari,

Almeaqli

et al. 2024

Journal of Pharmacy and Bioallied Sciences

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Objective: The present study aimed to conduct an assessment of parents’ knowledge, attitudes, and practices toward methamphetamine “shabu” abuse among youth and its risk factors. Materials and Methods: The present cross-sectional descriptive study was conducted on a sample of 1179 parents. Parents were assured that questionnaire content would stay classified and was given anonymously. It had 20 demographic, drug use, and addiction treatment questions. Statistical Package for Social Sciences v. 24 and Chi-Square test were used to examine the data after evaluating and coding it. Results: Out of a total of 1179 participants, only 11% had not heard about shabu, about 38% did not know the main symptoms of crystal addiction, and 46% did not know the long side effects of crystal addiction. The majority of participants mentioned that shabu is available in powder format (57%) or liquid (13%), while 27% did not know its form. Most of the participants (97%) think that the drug of shabu or crystal or ice is dangerous; about 60% of participants mentioned that there is an addict in the family. Conclusion: Parents have good knowledge levels regarding different aspects of methamphetamine or shabu abuse, symptoms, and its risk factors. Further in-depth studies are needed at whole Saudi Arabia.

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“…The claimed prevalence rates of substance misuse in Islamic nations are influenced by various factors. [ 10 11 12 13 14 ]…”

Section: Discussionmentioning

confidence: 99%

Parents’ Knowledge, Attitude, and Practices toward Methamphetamine Abuse among Youth and its Risk Factors in Saudi Arabia

Ibrahiem,

Al Dawsari,

Almeaqli

et al. 2024

Journal of Pharmacy and Bioallied Sciences

View full text Add to dashboard Cite

show abstract

“…Recent data suggest that methamphetamine use during pregnancy impairs differentiation of the ventral midbrain dopaminergic neurons by altering the expression of relevant genes involved in neurogenesis [36]. Moreover, recent studies demonstrated that H3K9me2mediated silencing is an important epigenetic regulator of gene expression throughout development [28], while a reduction in H3K9me2 levels and downregulation of G9A, a specific lysine 9 methyltransferase (K9MT), has been observed in the brain of mice repeatedly exposed to cocaine [37].…”

Section: Discussionmentioning

confidence: 99%

Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects

Ke,

Ambigapathy,

Ton

et al. 2023

IJMS

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The dopamine transporter (DAT) is an integral member of the dopaminergic system and is responsible for the release and reuptake of dopamine from the synaptic space into the dopaminergic neurons. DAT is also the major target of amphetamine (Amph). The effects of Amph on DAT have been intensively studied; however, the mechanisms underlying the long-term effects caused by embryonal exposure to addictive doses of Amph remain largely unexplored. As in mammals, in the nematode C. elegans Amph causes changes in locomotion which are largely mediated by the C. elegans DAT homologue, DAT-1. Here, we show that chronic embryonic exposures to Amph alter the expression of DAT-1 in adult C. elegans via long-lasting epigenetic modifications. These changes are correlated with an enhanced behavioral response to Amph in adult animals. Importantly, pharmacological and genetic intervention directed at preventing the Amph-induced epigenetic modifications occurring during embryogenesis inhibited the long-lasting behavioral effects observed in adult animals. Because many components of the dopaminergic system, as well as epigenetic mechanisms, are highly conserved between C. elegans and mammals, these results could be critical for our understanding of how drugs of abuse initiate predisposition to addiction.

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Amphetamine exposure during embryogenesis changes expression and function of the dopamine transporter in Caenorhabditis elegans offspring

Ambigapathy,

McCowan,

Carvelli

2024

Journal of Neurochemistry

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The dopamine transporter (DAT) is a transmembrane protein that regulates dopamine (DA) neurotransmission by binding to and moving DA from the synaptic cleft back into the neurons. Besides moving DA and other endogenous monoamines, DAT is also a neuronal carrier for exogenous compounds such as the psychostimulant amphetamine (Amph), and several studies have shown that Amph‐induced behaviors require a functional DAT. Here, we demonstrate that exposure to Amph during early development causes behavioral, functional, and epigenetic modifications at the Caenorhabditis elegans DAT gene homolog, dat‐1, in C. elegans offspring. Specifically, we show that, while embryos exposed to Amph generate adults that produce offspring with no obvious behavioral alterations, both adults and offspring exhibit an increased behavioral response when challenged with Amph. Our functional studies suggest that a decrease in DAT‐1 expression underlies the increased behavioral response to Amph seen in offspring. Moreover, our epigenetic data suggest that histone methylation is a mechanism utilized by Amph to maintain changes in DAT‐1 expression in offspring. Taken together, our data reveal that Amph, by altering the epigenetic landscape of DAT, propagates long‐lasting functional and behavioral changes in offspring.image

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The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain

Cited by 5 publications

References 49 publications

Parents’ Knowledge, Attitude, and Practices toward Methamphetamine Abuse among Youth and its Risk Factors in Saudi Arabia

Parents’ Knowledge, Attitude, and Practices toward Methamphetamine Abuse among Youth and its Risk Factors in Saudi Arabia

Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects

Amphetamine exposure during embryogenesis changes expression and function of the dopamine transporter in Caenorhabditis elegans offspring

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