The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats

Pavković, Željko; Milanović, Desanka; Ruždijić, Sabera; Kanazir, Selma; Pešić, Vesna

doi:10.1111/pan.13396

Cited by 14 publications

(12 citation statements)

References 24 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A distinction is made between habituation over time (intrasession) and over repeated exposures (intersession) [4]. In this original field of behavioral biology, the habituation process is the primary research objective and serves as a model for non-associative learning and memory mechanisms as well as for pharmacological studies [4][5][6]. One of the most frequently assessed parameters of habituation is a decrease in exploratory behavior measured in the Open Field (OF) test as a change in distance travelled or activity.…”

Section: Introductionmentioning

confidence: 99%

Repeatability analysis improves the reliability of behavioral data

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Repeatability analysis improves the reliability of behavioral data

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The consequences of general anesthetic exposure in infants and children have been well investigated and have demonstrated consequent cognitive and behavioral abnormalities (see: Lin et al, , for review). However, the impact of such exposures on later developmental periods, including adolescence, is very limited, but convergent evidence has been observed with respect to memory and learning (see: Landin et al, ; Pavkovic et al, ). To our knowledge, the present study is the first to assess the contribution of developmental anesthetic exposure on behaviors related to substance use disorder vulnerability using an animal model, whereas no human data have directly assessed this association.…”

Section: Discussionmentioning

confidence: 99%

General Anesthetic Exposure During Early Adolescence Persistently Alters Ethanol Responses

Landin

Gore-Langton

Spear

et al. 2020

Alcoholism Clin & Exp Res

View full text Add to dashboard Cite

Background Adolescent alcohol abuse can lead to behavioral dysfunction and chronic, relapsing alcohol use disorder (AUD) in adulthood. However, not all adolescents that consume alcohol will develop an AUD; therefore, it is critical to identify neural and environmental risk factors that contribute to increases in susceptibility to AUDs following adolescent alcohol (ethanol [EtOH]) exposure. We previously found that adolescent anesthetic exposure led to strikingly similar behavioral and neural effects as adolescent alcohol exposure. Therefore, we tested the hypothesis that general anesthetic exposure during early adolescence would alter EtOH responses consistent with an exacerbation of the adolescent alcohol phenotype. Methods To test this hypothesis, early‐adolescent male Sprague‐Dawley rats were exposed for a short duration to the general anesthetic isoflurane and tested on multiple EtOH‐induced behaviors in mid‐late adolescence or adulthood. Results Adolescent rats exposed to isoflurane exhibited decreases in sensitivity to negative properties of EtOH such as its aversive, hypnotic, and socially suppressive effects, as well as increases in voluntary EtOH intake and cognitive impairment. Select behaviors were noted to persist into adulthood following adolescent isoflurane exposure. Similar exposure in adults had no effects on EtOH sensitivity. Conclusions This study demonstrates for the first time that early‐adolescent isoflurane exposure alters EtOH sensitivity in a manner consistent with an exacerbation of adolescent‐typical alcohol responding. These findings suggest that general anesthetic exposure during adolescence may be an environmental risk factor contributing to an enhanced susceptibility to developing AUDs in an already vulnerable population.

show abstract

“…A distinction is made between habituation over time (intrasession) and over repeated exposures (intersession) (Leussis and Bolivar, 2006). In this original field of behavioral biology, the habituation process is the primary research objective and serves as a model for non-associative learning and memory mechanisms as well as for pharmacological studies (Leussis and Bolivar, 2006;Pavkovic et al, 2018;Evans et al, 2019). One of the most frequently assessed parameters of habituation is a decrease in exploratory behavior measured in the Open Field (OF) test as a change in distance travelled or activity.…”

Section: Introductionmentioning

confidence: 99%

Repeatability analysis improves the reliability of behavioral data

Rudeck

Vogl

Banneke

et al. 2019

Preprint

View full text Add to dashboard Cite

AbstractReliability of data has become a major concern in the course of the reproducibility crisis. Especially when studying animal behavior, confounding factors such as novel test apparatus can lead to a wide variability of data. At worst, effects of novelty or stress related behavior can mask treatment effects and the behavioral data may be misinterpreted. Habituation to the test situation is a common practice to circumvent novelty induced increases in variance and to improve the reliability of the respective measurements. However, there is a lack of published empirical knowledge regarding reasonable habituation procedures and a method validation seems to be overdue.This study aimed at setting up a simple strategy to increase reliability of behavioral data. Therefore, exemplary data from mice tested in an Open Field (OF) arena were used to elucidate the potential of habituation and how reliability of measures can be confirmed by means of a repeatability analysis using the software R. On seven consecutive days, male C57BL/6J, BALB/cJ and 129S1/SvImJ mice were tested in an OF arena once daily and individual mouse behavior (distance travelled, average activity) was recorded. A repeatability analysis was conducted in order to estimate the reliability of measured animal behavior with regard to repeated trials of habituation.Our data analysis revealed that monitoring animal behavior during habituation is important to determine when individual differences of the measurements are stable. Here, the mixed effect model framework proved to be a powerful tool for estimating repeatability values. Repeatability values from distance travelled and average activity increased over the habituation period, revealing that around 60 % of the variance of the data can be explained by individual differences between mice. The first day of habituation was statistically significantly different from the following 6 days in terms of distance travelled and average activity. A habituation period of three days appeared to be sufficient in this study. Overall these results emphasize the importance of habituation and in depth analysis of habituation data to define the correct starting point of the experiment for improving the reliability and reproducibility of experimental data.

show abstract

The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats

Cited by 14 publications

References 24 publications

Repeatability analysis improves the reliability of behavioral data

Repeatability analysis improves the reliability of behavioral data

General Anesthetic Exposure During Early Adolescence Persistently Alters Ethanol Responses

Repeatability analysis improves the reliability of behavioral data

Contact Info

Product

Resources

About