2010
DOI: 10.1016/j.biomaterials.2009.09.096
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The influence of rat mesenchymal stem cell CD44 surface markers on cell growth, fibronectin expression, and cardiomyogenic differentiation on silk fibroin – Hyaluronic acid cardiac patches

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Cited by 68 publications
(61 citation statements)
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“…37 MSC surface marker (CD44) is expressed widely by many stem-cell types and functions in cell adhesion, migration, homing, proliferation, survival, and apoptosis. 38,39 Mouse anti-HuNu (MAB1281; Millipore, Billerica, MA) and rabbit anti-CD44 (HPA005785; Sigma-Aldrich) were used to identify MSCs in tissues. The 4’,6-diamidino-2-phenylindole (DAPI) was used to identify nuclei, regardless of species, because it stains DNA.…”
Section: Methodsmentioning
confidence: 99%
“…37 MSC surface marker (CD44) is expressed widely by many stem-cell types and functions in cell adhesion, migration, homing, proliferation, survival, and apoptosis. 38,39 Mouse anti-HuNu (MAB1281; Millipore, Billerica, MA) and rabbit anti-CD44 (HPA005785; Sigma-Aldrich) were used to identify MSCs in tissues. The 4’,6-diamidino-2-phenylindole (DAPI) was used to identify nuclei, regardless of species, because it stains DNA.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore the biomaterials used in this approach should possess suitable nanostructures providing mechanical support and biological cues that promote cell attachment and growth. Chung et al investigated the effects of silk fibroin/chitosan and silk fibroin/chitosan/hyaluronic acid hybrid patches on cardiomyogenic differentiation of induced rat MSCs, and observed significant improvement in the expression of selected cardiac muscle genes (such as those encoding Tnnt2 and Acta1) and of selected cardiac proteins (such as cardiotin and connexin 43) on the cardiac patches incorporating GAG microspheres [136,137].…”
Section: Cardiac Regenerationmentioning
confidence: 98%
“…Thus, the ideal tissue constructs should stimulate stem cell differentiation into a cardiac lineage. Current cardiac tissue constructs are mostly microporous scaffolds and hydrogels based on natural polymers such as collagen [24-27] and polysaccharide [22,28,29], and synthetic polymers such as polyurethane [30,31] and poly(glycerol-sebacic acid) [23,24]. These scaffolds and hydrogels differ from the native myocardium, either structurally or mechanically, and are unable to produce a native-like microenvironment for stem cell differentiation and function [32].…”
Section: Introductionmentioning
confidence: 99%
“…These scaffolds and hydrogels differ from the native myocardium, either structurally or mechanically, and are unable to produce a native-like microenvironment for stem cell differentiation and function [32]. Studies based on these scaffolds have demonstrated that MSCs cannot readily differentiate into a cardiac lineage without using biochemicals such as 5-aza [22,29]. In this study we hypothesized that tissue constructs that mimic the structural and mechanical properties of the myocardium, may provide a native-like microenvironment for stimulating stem cell differentiation into a cardiac lineage.…”
Section: Introductionmentioning
confidence: 99%