THE INFLUENCE OF RATE AND TIME OF MINERAL N APPLICATION ON YIELD AND N<sub>2</sub> FIXATION BY FIELD BEAN

Kucey, R. M. N.

doi:10.4141/cjps89-054

Cited by 10 publications

(6 citation statements)

References 4 publications

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“…Inoculation with rhizobium inoculant Nitrastik-D increased N 2 -fixation of dry bean genotypes followed by Nodulator compared with uninoculated and 'N treatments (Table 3). On the other hand, application of N-fertilizer reduced nodulation and N 2 -fixation, as also reported by (Kucey 1989) in southern Alberta. N 2 -fixation strongly contributed to shoot N accumulation in some of the dry bean genotypes.…”

Section: Resultssupporting

confidence: 81%

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

et al. 2014

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(1 ). To identify efficient N 2 fixers, 22 dry bean genotypes were screened for symbiotic N 2 -fixation in potted low-N soil in the greenhouse under four treatments: (1) (1 ) with either rhizobial inoculant, and Viva fixed the highest amount (129 mg N plant(1 ) when inoculated with Nitrastik-D. In contrast, AC Black Diamond, Island, Winchester, AC Polaris, LEF 2RB and SEA 5 had low N 2 -fixing potential (3 to 23 mg N plant(1 ) in inoculated treatments. The qRT-PCR analysis indicated that nifH gene expression was 5 fold higher in GH-196 compared to the non-nodulating mutant R99, which further confirmed the high N 2 -fixation capacity of GH-196. After confirmation in the field, dry bean genotypes with improved N 2 -fixation potential identified in this study may be used as parents in breeding programs in the development of future cultivars. The nifH gene expression may be used as a rapid test to select dry beans with high N 2 -fixation potential. L'analyse qRT-PCR re´ve`le que l'expression du ge`ne nifH est cinq fois plus e´leve´e chez GH-196 que chez l'autre mutant (R99) ne produisant pas de nodules, ce qui confirme encore plus la forte capacite´de fixation du premier. Apre`s confirmation au champ, les ge´notypes de haricot a`meilleur potentiel de fixation de l'azote identifie´s dans le cadre de cette e´tude pourraient servir de parents dans les programmes d'hybridation visant a`cre´er de nouveaux cultivars. L'expression du ge`ne nifH pourrait servir de test pour se´lectionner rapidement les varie´te´s de haricot qui fixent bien le N 2 .Mots clé s: Se´lection de ge´notypes, inoculation, fixation du N 2 , Rhizobium, RT-PCR 1 Corresponding author (e-mail: newton.lupwayi@agr. gc.ca).Abbreviations: RDW, root dry weight; RT-PCR, reverse transcription polymerase chain reaction; SDW, shoot dry weight Can.

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Section: Resultssupporting

confidence: 81%

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

et al. 2014

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“…The occurrence of a severe N shortage in the plants treated with N-free NS despite inoculation with R. tropici CIAT899 during the first 3 to 5 weeks after inoculation is reasonable. Indeed, it is well known that a period of 3 to 5 weeks is needed between infection with N 2 -fixing microorganisms and the onset of N 2 fixation, and during this period, the plant has to provide N both to itself and to the bacteroids (Kucey, 1989;Marschner, 1995).…”

Section: Resultsmentioning

confidence: 99%

Responses of Hydroponically Grown Common Bean Fed with Nitrogen-free Nutrient Solution to Root Inoculation with N2-fixing Bacteria

Kontopoulou¹,

Giagkou²,

Stathi³

et al. 2015

horts

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To date, few attempts have been made to assess the impact of Rhizobium inoculation on N2 fixation and plant yield in soilless cultivations of common bean. In the present study, common bean (P. vulgaris L.) grown on an inert medium (pumice) was inoculated with either Rhizobium tropici CIAT899 or a commercial product containing a mix of N2-fixing bacteria, specifically rhizobia, and Azotobacter sp. The plants treated with both inoculants were supplied with nitrogen (N)-free (0% N) nutrient solution (NS) throughout the cropping period. A third treatment with non-inoculated plants, which were supplied with a standard (100% N) NS was applied as a control. Inoculation with R. tropici significantly increased the total number of root nodules (80 nodules per plant on average) in comparison with the other two treatments (nine nodules per plant on average). The supply of N-free NS restricted markedly both total plant biomass and pod yield, whereas the inoculation with R. tropici mitigated this effect. The aboveground tissues of plants fed with N-free NS contained appreciably less N than those fed with standard solution when they were inoculated with the commercial inoculant (1.7 vs. 29 mg·g−1 dry weight, respectively). The shoot total N concentration 45, 65, and 90 days after transplanting (32, 31, and 29 mg·g−1 dry weight, respectively) was not reduced by the supply of N-free NS when the plants were inoculated with R. tropici. This finding indicates that, at least from the first sampling date onward, the tissue N level was not a limiting factor for growth and yield in plants inoculated with R. tropici. The supply of N-free NS restricted appreciably the potassium (K), magnesium (Mg), and zinc (Zn) levels in the aboveground plant biomass, regardless of inoculation treatment. The impaired growth and yield in plants fed with N-free NS and inoculated with R. tropici is ascribed to both a N shortage at early growth stages and a reduced K+ uptake aimed at electrochemically balancing the anion-to-cation uptake ratio under conditions of no external NO3– supply.

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“…However, fertilizer rates exceeding those exerting a "starter nitrogen" effect generally reduce nodulation and N 2 fixation (11). The response of the Rhizobium-legume symbiosis to added nitrogen fertilizer is definitely determined by time of application (growth stage), level and form of N, and the legume species (8,160,182). Nitrates are more inhibitory to nodulation than is ammonia, especially if added shortly after planting.…”

mentioning

confidence: 99%

Rhizobium -Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate

Zahran

1999

Microbiol Mol Biol Rev

1,370

635

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SUMMARY Biological N2 fixation represents the major source of N input in agricultural soils including those in arid regions. The major N2-fixing systems are the symbiotic systems, which can play a significant role in improving the fertility and productivity of low-N soils. The Rhizobium-legume symbioses have received most attention and have been examined extensively. The behavior of some N2-fixing systems under severe environmental conditions such as salt stress, drought stress, acidity, alkalinity, nutrient deficiency, fertilizers, heavy metals, and pesticides is reviewed. These major stress factors suppress the growth and symbiotic characteristics of most rhizobia; however, several strains, distributed among various species of rhizobia, are tolerant to stress effects. Some strains of rhizobia form effective (N2-fixing) symbioses with their host legumes under salt, heat, and acid stresses, and can sometimes do so under the effect of heavy metals. Reclamation and improvement of the fertility of arid lands by application of organic (manure and sewage sludge) and inorganic (synthetic) fertilizers are expensive and can be a source of pollution. The Rhizobium-legume (herb or tree) symbiosis is suggested to be the ideal solution to the improvement of soil fertility and the rehabilitation of arid lands and is an important direction for future research.

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THE INFLUENCE OF RATE AND TIME OF MINERAL N APPLICATION ON YIELD AND N₂ FIXATION BY FIELD BEAN

Cited by 10 publications

References 4 publications

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Responses of Hydroponically Grown Common Bean Fed with Nitrogen-free Nutrient Solution to Root Inoculation with N2-fixing Bacteria

Rhizobium -Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate

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THE INFLUENCE OF RATE AND TIME OF MINERAL N APPLICATION ON YIELD AND N2 FIXATION BY FIELD BEAN

Cited by 10 publications

References 4 publications

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Responses of Hydroponically Grown Common Bean Fed with Nitrogen-free Nutrient Solution to Root Inoculation with N2-fixing Bacteria

Rhizobium -Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate

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THE INFLUENCE OF RATE AND TIME OF MINERAL N APPLICATION ON YIELD AND N₂ FIXATION BY FIELD BEAN