The influence of rotating magnetic field on bio-catalytic dye degradation using the horseradish peroxidase

Wasak, Agata; Drozd, Radosław; Jankowiak, Dorota; Rakoczy, Rafał

doi:10.1016/j.bej.2019.04.007

Cited by 22 publications

(16 citation statements)

References 30 publications

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“…Namely, background electromagnetic radiation is concentrated upon its reflection from the interior surface of the half-sphere, leading to a change in the spatial topology of this radiation. Electromagnetic fields are known to influence proteins [ 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. Previously, we demonstrated that even ultra-weak electromagnetic fields, whose intensity is comparable to that of the background (of 10 −12 W/cm 2 power density) electromagnetic fields of a non-standard specific topology—such as knotted electromagnetic fields—can induce substantial alterations in protein adsorption properties [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

“…In modern life, electromagnetic fields are widely employed, influencing living organisms. The functionality of protein systems (including enzymatic ones) can be altered under the action of magnetic [ 2 ] and electromagnetic fields [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. In this way, in previous studies, we demonstrated that electric fields, triboelectrically induced by liquid flow through polymeric pipes of thermal stabilization coils, influence the adsorbability of the horseradish peroxidase (HRP) enzyme protein onto mica substrates [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…As regards proteins, alterations in their functionality can manifest themselves not only as direct changes in functional activity [ 2 , 3 , 9 ], but also in the form of changes in adsorbability [ 5 , 6 , 7 , 10 ] onto functional surfaces of biosensors and bioreactors. The latter is of importance for biotechnology applications, where bioreactors with surface-immobilized enzymes are widely employed [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Spherical Elements of Biosensors and Bioreactors on the Physicochemical Properties of a Peroxidase Protein

Ivanov

Tatur²,

Pleshakova

et al. 2021

Polymers

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External electromagnetic fields are known to be able to concentrate inside the construction elements of biosensors and bioreactors owing to reflection from their surface. This can lead to changes in the structure of biopolymers (such as proteins), incubated inside these elements, thus influencing their functional properties. Our present study concerned the revelation of the effect of spherical elements, commonly employed in biosensors and bioreactors, on the physicochemical properties of proteins with the example of the horseradish peroxidase (HRP) enzyme. In our experiments, a solution of HRP was incubated within a 30 cm-diameter titanium half-sphere, which was used as a model construction element. Atomic force microscopy (AFM) was employed for the single-molecule visualization of the HRP macromolecules, adsorbed from the test solution onto mica substrates in order to find out whether the incubation of the test HRP solution within the half-sphere influenced the HRP aggregation state. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) was employed in order to reveal whether the incubation of HRP solution within the half-sphere led to any changes in its secondary structure. In parallel, spectrophotometry-based estimation of the HRP enzymatic activity was performed in order to find out if the HRP active site was affected by the electromagnetic field under the conditions of our experiments. We revealed an increased aggregation of HRP after the incubation of its solution within the half-sphere in comparison with the control sample incubated far outside the half-sphere. ATR-FTIR allowed us to reveal alterations in HRP’s secondary structure. Such changes in the protein structure did not affect its active site, as was confirmed by spectrophotometry. The effect of spherical elements on a protein solution should be taken into account in the development of the optimized design of biosensors and bioreactors, intended for performing processes involving proteins in biomedicine and biotechnology, including highly sensitive biosensors intended for the diagnosis of socially significant diseases in humans (including oncology, cardiovascular diseases, etc.) at early stages.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Spherical Elements of Biosensors and Bioreactors on the Physicochemical Properties of a Peroxidase Protein

Ivanov

Tatur²,

Pleshakova

et al. 2021

Polymers

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“…Although the mechanisms underlying magnetic effects have not been thoroughly revealed, magnetic fields have been used in medical aid therapy [54] , [55] , [56] , [57] and in the food industry for sterilization [24] , [58] , [59] , [60] , [61] , [43] , passivation of enzyme activity [45] , [62] , preservation of fruits and vegetables [63] , [64] , [65] , [66] , auxiliary microbial fermentation ( Fig. 2 ) [67] , [68] , [69] , [70] , auxiliary enzymatic reactions [71] , component separation and extraction [72] , [73] , [74] , [75] , strain mutagenesis [76] , [77] , quality enhancement [78] , [79] , [80] , improvement of seed growth and physiological properties [81] , and reduction of microbial adsorption of heavy metals [82] . Other research fields have used magnetic fields for a wide range of applications [83] , [84] .…”

Section: Physical Fieldsmentioning

confidence: 99%

Prospects and application of ultrasound and magnetic fields in the fermentation of rare edible fungi

Wen

et al. 2021

Ultrasonics Sonochemistry

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“…Among the main advantages of the use of peroxidases in industrial process is the ecologic approach that contributes to the development of environmentally sustainable processes [20] and high specificity and high selectivity for substrates [21]. However, the industrial applications of the enzymes in free form are limited due to the impossibility of reuse and instability, as for example, at higher temperatures and pHs that are not equal to the physiological environment, low stability in organic solvents and storage [21][22][23][24]. Enzyme immobilization can provide stability to the biocatalyst.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Low-Cost Alternative Vegetable Peroxidase (Raphanus sativus L. peroxidase): Choice of Support/Technique and Characterization

et al. 2020

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In the present work the radish (Raphanus sativus L.) was used as the low-cost alternative source of peroxidase. The enzyme was immobilized in different supports: coconut fiber (CF), calcium alginate microspheres (CAMs) and silica SBA-15/albumin hybrid (HB). Physical adsorption (PA) and covalent binding (CB) as immobilization techniques were evaluated. Immobilized biocatalysts (IBs) obtained were physicochemical and morphologically characterized by SEM, FTIR and TGA. Also, optimum pH/temperature and operational stability were determined. For all supports, the immobilization by covalent binding provided the higher immobilization efficiencies—immobilization yield (IY%) of 89.99 ± 0.38% and 77.74 ± 0.42% for HB and CF, respectively. For CAMs the activity recovery (AR) was of 11.83 ± 0.68%. All IBs showed optimum pH at 6.0. Regarding optimum temperature of the biocatalysts, HB-CB and CAM-CB maintained the original optimum temperature of the free enzyme (40 °C). HB-CB showed higher operational stability, maintaining around 65% of the initial activity after four consecutive cycles. SEM, FTIR and TGA results suggest the enzyme presence on the IBs. Radish peroxidase immobilized on HB support by covalent binding is promising in future biotechnological applications.

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The influence of rotating magnetic field on bio-catalytic dye degradation using the horseradish peroxidase

Cited by 22 publications

References 30 publications

Effect of Spherical Elements of Biosensors and Bioreactors on the Physicochemical Properties of a Peroxidase Protein

Effect of Spherical Elements of Biosensors and Bioreactors on the Physicochemical Properties of a Peroxidase Protein

Prospects and application of ultrasound and magnetic fields in the fermentation of rare edible fungi

Immobilization of Low-Cost Alternative Vegetable Peroxidase (Raphanus sativus L. peroxidase): Choice of Support/Technique and Characterization

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