The Influence of Selected Flavonoids from the Leaves of Cirsium palustre (L.) Scop. on Collagen Expression in Human Skin Fibroblasts

Abstract

Ten flavonoids belonging to the subclasses of flavones, flavanones and aurones were isolated from methanolic extract of Cirsium palustre leaves after multistep chromatographic separation. Their structures were elucidated with spectroscopic methods. All compounds, except for luteolin 7-O-glucoside, were isolated for the first time. Four compounds-eriodictyol 7-O-glucoside (6), 6-hydroxyluteolin 7-O-glucoside (11), scutellarein 7-O-glucoside (12) and pedalitin (14)-were tested for their effect on collagen expression in normal human dermal fibroblasts. Among them, compound 11 at 40 μM and compound 14, at all concentrations used (1, 20, 40 μM), significantly enhanced the level of total collagen secreted into the medium. Furthermore, compound 11 significantly stimulated type I collagen expression, whereas compound 14 activated type I and III collagen expression at the mRNA level, depending on concentration. MMP-2 activity was inhibited by all study compounds, with the greatest effect recorded with compound 14 at 20 μM. The lack of effect on collagen content in the medium of compound 6- and compound 12-treated cells, besides an increase in COL1A1 and COL1A2 expression, might be caused by diminished expression of HSP47 gene, resulting in decreased procollagen secretion. Future study of compounds 11 and 14 for their potential therapeutic use in conditions connected with collagen biosynthesis deficiency is required.

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

“…Skin aging manifests wrinkles, diminished structural integrity and impaired wound healing due to alterations in the extracellular matrix formed predominantly of Colla1 (Jenkins, 2002;Watson et al, 2009). Furthermore, it has been reported that the expression of MMP1 in dermal fibroblasts is induced under oxidative stress conditions (Buechner et al, 2008;Nazaruk and Galicka, 2014). Interestingly, MMP1R transcripts were found to be drastically down-regulated in H 2 O 2 stressed cells pre-treated NannoG, in contrast to only extract-treated control cells.…”

Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models

“…These parameters indicate if the wound closure follows a normal process, in which the newly formed tissue gradually develops drastic structural changes to reconstitute the morphofunctional characteristics of the intact skin. Works which demonstrated the importance of these analyses assert that the type and quantity of collagen fibers deposited on the tissue can be used as a marker of tissue mechanical resistance [2, 3, 9, 58]. The connective and epithelial tissues form a support structure to promote the correct closure of the wound [57, 63], reducing the chances of opportunistic infections in the wounded area [38, 64].…”

“…The inflammatory phase comprehends the intense leucocytes recruitment to the wound area, removal of cellular and extracellular matrix debris, and syntheses of regulatory molecules such as cytokines and chemokines [1, 2]. The proliferative phase progresses with an intense proliferation and migration of fibroblasts, endothelial cells, and keratinocytes as well as formation of the granulation tissue (rich in type III collagen) and progressive reepithelialization [1–3].…”

“…The proliferative phase progresses with an intense proliferation and migration of fibroblasts, endothelial cells, and keratinocytes as well as formation of the granulation tissue (rich in type III collagen) and progressive reepithelialization [1–3]. At the maturation phase, type III collagen is gradually replaced by type I collagen, which originates more thicker and resistant collagen fibers [2–4]. …”

Ethno-Herbal-Medico in Wound Repair: An Incisive Review

“…Moreover, they explored the potentiality of the proanthocyanidin extract on cutaneous wound healing in vivo and claimed that the grape seed extract bioactive component enhances the organization of connective tissue and improved histological profile. Nazaruk and Galicka () isolated 10 flavonoids (subclasses of flavones, flavanones and aurones) from methanol extract of Cirsium palustre leaves. The flavonoids are tested for the effect on collagen expression in HDFs.…”