The influence of selection on reaction to stress in mice V. The effect of starvation and glutathione injection on the activity of N‐acetyl‐beta‐glucosaminidase and the level of glutathione in the liver

Abstract

Summary The experiment was conducted on 40 6‐week‐old mice selected for a high rate of body weight gain and 40 unselected controls. Model stressors were used: starvation for 24 h, injection of glutathione and both stressors jointly. Measurements were made of N‐acetyl‐beta‐glucosaminidase (NAGL) activity and the level of non‐protein sulfhydryl groups in the liver. Starvation, glutathione injection and starvation with glutathione injection jointly caused an increase of NAGL activity, which was more marked in the selected mice. Starvation caused a decrease of glutathione level, glutathione injection and both stressors jointly caused an increase in the level of glutathione. The reactions of selected mice were stronger in comparison with unselected ones. The results suggest that the mice selected for a high rate of body weight gain were less resistant to starvation and glutathione injection than the unselected mice. Zusammenfassung Der Einfluβ der Selektion auf die N‐Azetyl‐β‐glukosaminidase Aktivität und Glutathionniveau in der Leber der Mäuse Es wurden 40 Mäuse im Alter 6 Wochen einer Selektionslinie auf Körpergewicht und 40 Mäuse der Kontrolle untersucht. Den Tieren der Versuchsgruppe wurden die Nahrung 24 Stunden entzogen. Die Glutathioninjektion und Hungern zusammen mit Glutathioninjektion wurde auch eingesetzt. Es wurde Leber zur Bestimmung der Aktivität von N‐Azetyl‐β‐glukosaminidase und des Glutathionniveau entnommen. Die Ergebnisse zeigen, daβ selektierte Tiere empfindlicher als Kontrolltiere gegen Belastungen gewesen sind.

The influence of selection on reaction to stress in mice.VII. Activity of some lysosomal enzymes in the muscle, spleen, kidney and liver

“…This experiment continued previous studies ( Witek 1998 ; Koątaj et al . 1992 , 1995, 1996; Rysińska et al .…”

“…The earlier studies ( Ko&aogon,taj et al . 1995 , 1996; Witek & Ko&aogon,taj 1998) showed that selection for a high rate of body weight gain can change the biochemical reactivity of an organism in connection with damaging environmental factors. The conclusions are similarto those that can be inferred from the papers of Gunselt et al .…”

Influence of starvation and sex on some lysosomal enzymes activity in young dairy cattle

“…Our earlier studies revealed that the activity of the degradation system of the lysosomal compartment is a significant factor maintaining the animal cell in the state of dynamic homeostasis, as well as an important indicator of stress reactivity. Observations conducted on pigs (KOŁĄTAJ et al, 1996), cattle (KOŁĄTAJ et al, 1998), sheep (KOŁĄTAJ et al, 2002 as also on mice (JÓŹWIK et al, 2003 a b;LANKOFF and KOŁĄTAJ, 2001;WITEK and KOŁĄTAJ, 1998, 2001), rabbits (KONECKA et al, 2002 and quail confirmed this hypothesis. The data obtained from them indicate that the stress factors, employed in these studies, caused the significant changes in the reactivity of the animal cell lysosomal system.…”

“…In connection with these data, it seemed interesting to estimate the changes of the activity of some model lysosomal enzymes in the blood plasma of young bulls and heifers maintained in farm conditions, fed normally and starved for a short period. Fasting or restriction of food are a specific types of stress burden, which appears quite often in domestic animals (OHSHITA et al, 1986;WITEK and KOŁĄTAJ, 1998). The aim of this study was to determine the influence of starvation and sex on the activity of some lysosomal enzymes in young cattle.…”

The influence of selection on reaction to stress in mice. IX. Effect of dietary protein level on activity of lysosomal enzymes in liver and kidney

“…The results obtained suggest that selected mice, receiving both types of diets, have as a rule higher enzyme activity than unselected control animals. The studies of Witek and Kołątaj (1998) and Kołątaj et al (1998) indicate that the lysosomal system is significantly connected with homeostasis. Homeostatic disturbances caused by prolonged selection may also be observed in the activity of non‐lysosomal enzymes ( Kołątaj et al 1995, 1996).…”