The influence of selenium substitution on microcirculation and glutathione metabolism after warm liver ischemia/reperfusion in a rat model

Zapletal, C.; Heyne, S.; Breitkreutz, Raoul; Gebhard, Martha-Maria; Golling, M.

doi:10.1016/j.mvr.2008.04.005

Cited by 22 publications

(13 citation statements)

References 32 publications

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“…Supplementation of sodium selenite for 3 days is also able to enhance ACh-induced aortic ring relaxation by increasing NO release in rats [33], mainly due to selenoproteins existing in the arterial wall [25]. Neutralizing deleterious radicals with continuous supplementation of Se in Se-deficient animals has been shown to protect the microcirculation and improve vascular functions [34]. Therefore, the aim of this study was to evaluate the effects of dietary Se supplementation on microcirculatory parameters of fructose-fed animals.…”

Section: Discussionmentioning

confidence: 99%

Effects of Selenium in the Microcirculation of Fructose-Fed Hamsters

Castiglione¹,

Barros²,

Boa³

et al. 2018

J Vasc Res

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Background and Aims: Fructose intake is directly related to vascular dysfunction and it is a risk factor for the development of metabolic and cardiovascular diseases, such as obesity, diabetes, and hypertension. Selenium, a component of antioxidant enzymes, improves hyperglycemia and vascular function in diabetic animals. The aim of this study was to evaluate the effects of dietary selenium supplementation on microcirculatory and metabolic parameters of fructose-fed hamsters. Methods and Results: Male hamsters (Mesocricetus auratus) had their drinking water substituted or not by 10% fructose solution for 60 days, during which their microcirculatory function was evaluated in the cheek pouch preparation. Blood glucose and serum insulin levels were also tested. Microcirculatory responses to acetylcholine (an endothelium-dependent vasodilator) and to sodium nitroprusside (SNP, an endothelium-independent vasodilator), and macromolecular permeability increase induced by a 30-min ischemia/reperfusion (I/R) procedure, showed that endothelium-dependent and independent vasodilatation was significantly increased in animals that had high selenium supplementation, in both the control and fructose-fed groups. Selenium supplementation protected against plasma leakage induced by I/R in all control and fructose-fed groups. Conclusion: Our results indicate that dietary selenium supplementation reduces microvascular dysfunction by increasing endothelial-dependent and independent dilatation and reducing macromolecular permeability increase in fructose-fed animals.

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Section: Discussionmentioning

confidence: 99%

Effects of Selenium in the Microcirculation of Fructose-Fed Hamsters

Castiglione¹,

Barros²,

Boa³

et al. 2018

J Vasc Res

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“…71 More importantly, further attempts should be made by regulatory authorities and transplant societies to standardize procurement protocols and techniques, with the goal of reducing the inherent problems associated with DCD liver grafts. Increased attention and initiatives to promote DCD as a strategy to enlarge the donor pool (not at the cost of DBD) remain crucially important.…”

Section: Discussionmentioning

confidence: 99%

“…66 In addition, other biomarkers, including xanthine, hypoxanthine, hyaluronic acid, and reduced glutathione have been suggested for the assessment of graft viability. [67][68][69][70][71] Therefore, evaluating these biomarkers in DCD liver grafts prior to implant would be helpful. However, some technical limitations (such as the inability to identify these biomarkers in peripheral body fluids), technical demands, and time constraints in the clinical setting preclude these biomarkers from being incorporated into current practice.…”

Section: Figure 2 Ex Vivo Normothermic Machine Perfusion Systemmentioning

confidence: 99%

Untitled

2015

Exp Clin Transplant

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There are increasing numbers of patients on liver transplant waiting lists, and there is a continuing organ shortage crisis. Therefore, more centers and organ procurement organizations are developing protocols for donation after cardiac death. However, the effect of donation after cardiac death allografts on overall patient survival remains controversial, with some centers reporting equivalent patient posttransplant survival but many others indicating increased rates of complications, retransplant, utilization of resources, and death. Several potential risk factors that predict graft loss and recipient complications have been identified. To improve patient outcomes and reduce dropouts, experimental strategies that target both donors and recipients at various phases of the transplant process have focused on attenuating ischemia-reperfusion injury and have achieved encouraging results. In the present article, our goal is to provide an overview of the current status of, and recent advances in, liver transplant from donation after cardiac death, to better understand the risks and potential benefits of donation after cardiac death liver transplant.

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“…Among all, hydrophilic ascorbic acid (vitamin C) and lipophilic α -tocopherol (vitamin E) are the important components of the human antioxidant system [42]. Carotenoids, the principal dietary source of vitamin A in humans [43], polyphenolic compounds in green tea extract [36, 44], selenium [45], and zinc [46] all exert antioxidant action; a synergism among the different antioxidants as part of an antioxidant network has been shown [47–49]. …”

Section: Discussionmentioning

confidence: 99%

Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia

Nickkholgh

et al. 2012

HPB Surgery

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Background. Several approaches have been proposed to pharmacologically ameliorate hepatic ischemia/reperfusion injury (IRI). This study was designed to evaluate the effects of a preconditioning oral nutritional supplement (pONS) containing glutamine, antioxidants, and green tea extract on hepatic warm IRI in pigs.Methods. pONS (70 g per serving, Fresenius Kabi, Germany) was dissolved in 250 mL tap water and given to pigs 24, 12, and 2 hrs before warm ischemia of the liver. A fourth dose was given 3 hrs after reperfusion. Controls were given the same amount of cellulose with the same volume of water. Two hours after the third dose of pONS, both the portal vein and the hepatic artery were clamped for 40 min. 0.5, 3, 6, and 8 hrs after reperfusion, heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), portal venous flow (PVF), hepatic arterial flow (HAF), bile flow, and transaminases were measured. Liver tissue was taken 8 hrs after reperfusion for histology and immunohistochemistry.Results. HR, MAP, CVP, HAF, and PVF were comparable between the two groups. pONS significantly increased bile flow 8 hrs after reperfusion. ALT and AST were significantly lower after pONS. Histology showed significantly more severe necrosis and neutrophil infiltration in controls. pONS significantly decreased the index of immunohistochemical expression for TNF-α, MPO, and cleaved caspase-3 ().Conclusion. Administration of pONS before and after tissue damage protects the liver from warm IRI via mechanisms including decreasing oxidative stress, lipid peroxidation, apoptosis, and necrosis.

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The influence of selenium substitution on microcirculation and glutathione metabolism after warm liver ischemia/reperfusion in a rat model

Cited by 22 publications

References 32 publications

Effects of Selenium in the Microcirculation of Fructose-Fed Hamsters

Effects of Selenium in the Microcirculation of Fructose-Fed Hamsters

Untitled

Effects of a Preconditioning Oral Nutritional Supplement on Pig Livers after Warm Ischemia

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