The influence of size or number of biopsies on rapid urease test results: A prospective evaluation

Laine, Loren; Chun, Dae Chul; Stein, Craig; El-Beblawi, Ihab; Sharma, Vishwinder; Chandrasoma, Parakrama

doi:10.1016/0016-5085(95)23232-x

Cited by 12 publications

(14 citation statements)

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“…The early observation that H. pylori produces large amounts of urease activity led to the development of methods for the indirect detection of the organism in gastric biopsy tissue. The sensitivity of all urease-based tests for detection of H. pylori is dependent upon the bacterial load in the stomach (185). The CLOtest (Delta West Ltd., Bentley, Australia), developed by Marshall, was the first of the commercially available biopsy urease tests designed specifically for H. pylori detection.…”

Section: Methods Requiring Endoscopymentioning

confidence: 99%

“…The test is interpreted up to 24 h after placement of the gastric biopsy sample onto the agar gel. Of the available biopsy urease tests (also known as rapid urease tests) the CLOtest has been the most widely studied (73,185,187).…”

Section: Methods Requiring Endoscopymentioning

confidence: 99%

“…Biopsy-based methods. Since the sensitivity of the CLOtest is dependent on the density of H. pylori (185), it is reasonable to expect that biopsy urease tests may be less sensitive in the posttreatment setting. To minimize sampling errors, especially for rapid urease testing, multiple biopsy specimens should be taken (108).…”

Section: Posttreatment Diagnosismentioning

confidence: 99%

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Helicobacter pylori.

Dunn¹,

Cohen²,

Blaser³

1997

Clinical Microbiology Reviews

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Section: Methods Requiring Endoscopymentioning

confidence: 99%

Section: Methods Requiring Endoscopymentioning

confidence: 99%

See 1 more Smart Citation

Helicobacter pylori.

Dunn¹,

Cohen²,

Blaser³

1997

Clinical Microbiology Reviews

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“…Yousfi et al demonstrated that the diagnostic yield for the detection of H. pylori by rapid urease test was not affected by the size of biopsy forceps 6 . In contrast, Laine et al reported that increasing the amount of biopsied specimen in the CLOtest (Delta West, Bently, Australia) did significantly shorten the time to obtain a positive result 7 …”

Section: Biopsy Site Number and Biopsy Forcepsmentioning

confidence: 99%

RECENT PROGRESS IN ENDOSCOPY‐BASED DIAGNOSIS OF HELICOBACTER PYLORI INFECTION

Satô

Fujino

Kitahara

et al. 2001

Digestive Endoscopy

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Recently the Japanese Society for Helicobacter Research (JSHR) published the guideline for the diagnosis and treatment of H. pylori infection. 2 It is estimated that the Japanese Ministry of Health and Welfare will approve the diagnosis and treatment of H. pylori infection as the health insurancecovered medical services in the near future. The aim of this paper is to review current endoscopy-based diagnostic modalities for H. pylori infection and to emphasize their preferential indications, taking into account the particular settings in which a diagnosis is to be made. BIOPSY SITE, NUMBER AND BIOPSY FORCEPSIt is now well-recognized that persistent H. pylori infection develops atrophic gastritis leading to intestinal metaplasia. Intestinal metaplasia and patchy distribution of the bacteria are thought to be factors causing false-negative diagnoses of H. pylori infection. There are several reports regarding biopsy sites and number for the accurate diagnosis of H. pylori infection. Hazzel et al. reported that it was necessary to take biopsies from both the antrum and the body, as H. pylori might be found in either of them. 5 Genta and Graham reported that two antral biopsy specimens, one from lesser curvature and one from greater curvature, yield virtually 100% sensitivity for the histological detection of H. pylori infection. They also recommended three biopsy specimens (one from each of lesser curvature and greater curvature of the antrum and greater curvature of the corpus) as a routine evaluation of H. pylori infection, because no patients having extensive atrophic gastritis with intestinal metaplasia were included in their series. 3 Satoh et al. indicated that the Digestive Endoscopy (2001) 13, 3-6Numerous invasive and non-invasive tests are available in the detection of Helicobacter pylori. Endoscopy-based tests that include rapid urease test, histological examination and culture are important generally in the assessment of H. pylori status before eradication therapy. Recently, several new endoscopy-based diagnostic methods have been developed aiming at rapid and accurate detection of the organisms. It would be possible to diagnose H. pylori infection in treated patients by using these new highly sensitive tests. Although the diagnosis of H. pylori infection itself is possible by using non-invasive diagnostic tests, endoscopy-based tests provide not only the diagnosis of the organisms, but also the exclusive information such as treatment indications and the susceptibility for the antimicrobial drugs. Recently, new triple therapy including clarithromycin has been widely performed in Japan. Along with an increase in the prevalence of the antibiotic-resistant strains, culture may become a more important diagnostic method in the future. The inappropriate application of the tests may increase the potential risk of the misdiagnosis and the treatment failures. The diagnostic method should be selected by taking into account the circumstances in which a diagnosis is to be performed.

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“…Several studies have shown that specimen size does not adversely affect the diagnostic yield of a rapid urease test, when specimens are taken with 1.8 mm and 3.3 mm forceps, and with 2.2 mm and 3.3 mm forceps 4–6 . But, no direct comparative study has been conducted between 1.8 mm and 2.2 mm biopsy forceps.…”

Section: Introductionmentioning

confidence: 99%