“…Studies involving growth requirements of thermophilic bacilli have suggested the fastidiousness of these organisms, including a definite methionine requirement (Baker _et al", 1960) and the necessity of nitrate ion in supporting sporula tion (Dahl, 1955). Many reports in the literature dealing with the effect of temperature on the growth requirements of microorganisms have shown that as the incubation temperature is increased there is an increase in the growth requirements of the particular organism under study.…”