The Influence of Spirulina platensis on Physiological Characterization and Mitigation of DNA Damage in Salt-stressed Phaseolus vulgaris L. Plants

Abstract

S ALINITY severely reduced crop productivity. In this work, the effectiveness of Spirulina platensis (100mg/L) as a foliar growth stimulator was tested for its ability to reduce the harmful effect of salinity on Phaseolus vulgaris L. plants. The experiment was divided into four groups as follows: G 1 (control), G 2 (200mM NaCl), G 3 (100mg/L of Spirulina platensis), and G 4 (200mM NaCl + 100mg/L of Spirulina platensis). The results declared that shoot weight, plant height, leaves number, during the vegetative growth stage, pods number/plant, seed number/ pod, pods weight during fruiting stage, as well as content of carotenoids, chlorophyll a+b, 100seed weights, photosynthetic activity (assimilation of 14 CO 2 ), and total protein content reduced considerably in salt-stressed Phaseolus vulgaris. When compared to salt-stressed plants, the above metrics were enhanced by G 4 . In comparison to the control, the presence of salinity increased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), peroxidase activity (POD), malondialdehyde (MDA), free proline, transpiration rate, and total phenol. Additionally, compared to plants under salt stress, G 4 application (200mM NaCl + 100mg/L of Spirulina platensis) decreased the aforementioned metrics. Treatments of Phaseolus vulgaris with 200mM NaCl + 100mg/L of Spirulina platensis considerably increased the macronutrient content and decreased the Na + and Cl − levels as compared to G 2 . Obtained results proved that foliar applications of S. platensis at 100 mg/l have a high potential for improving growth, photosynthetic capacity, yield production, decreased ROS-induced oxidative damage, and reducing DNA damage in salt-stressed Phaseolus vulgaris.

Enhancing french basil growth through synergistic Foliar treatment with copper nanoparticles and Spirulina sp.

“…Additionally, foliar fertilizers aid in preventing toxicity symptoms that could develop after applying the same microelements to the soil [20]. In this research we found that plant height and the number of leaves were increased with the combination of CuNPs with biofertilizer (spirulina) at various concentrations therefore, the spraying of 500 mg/L of CuNPs + 1.5 g/L algae extract produced significantly best plant height and a number of branches in both seasons as follows (51.567, 51.900 cm/plant) (9.00, 10.00 /plant), respectively.These findings were in line with those of [59], who proved that the effectiveness of the combined treatment of 200 mM NaCl with 100 mg/L of Spirulina platensis enhanced growth with increasing photosynthetic pigment content and plant yield, which is due to reduced ROS-induced oxidative damage and decreased the damage to DNA in salt-stressed Phaseolus vulgaris compared with treatments of salt stress without spirulina. Similarly [60], who found the application of 192 kg of fertilizer N/ha combined with destructed spirulina at 6, 12, or 18 g cells/ha on cotton, recorded the highest number of open bolls and, additionally, the best plant height and fiber length.…”

“…On the contrary, foliar application of biofertilizer S. platensis (100 mg/L) on Phaseolus vulgaris plants under salinity stress achieved decreasing DNA damage along with ROS-induced oxidative damage. Thus, the growth improvement of S. platensis is due to its bioactive compounds, plant growth regulators, which scavenge ROS; therefore, algal application has been used in recent sustainable agriculture to decrease stress hazards [59].…”

B- Alleviating the harmful impact of salinity stress with different levels by using some organic techniques on Chemical characteristics of Wonderful cv. Pomegranate Transplants.

“…Also, reduces soil salinity and improves soil structure by increasing drainable pores and aggregates thus improving irrigation water filtration. Recently, (Taha et al, 2023) confirmed that Spirulina algae has many advantages in increasing soil fertility by increasing K+ and N accumulation, which led to the accumulation of several elements because of its high binding ability and synthesis salt stress proteins.…”

“…While, Cyanobacteria algae has the ability to fixing the atmospheric N 2 and the transformation into an accessible form of ammonia, mobilize insoluble inorganic phosphate forms, decrease Na + levels and its strength in bioaccumulation of several micro elements which related to its high binding ability and synthesis salt stress proteins for many cultivated plants (Taha et al, 2023). -Total carbohydrates, total phenols and proline content % in dry leaves: Table (6) shows that the contents of carbohydrate, phenols and proline % were increased with increasing salinity levels, the lowest results were recorded when irrigating the transplants with 6000 followed by 7000 and then 8000 ppm in both seasons compare to the controls T 1 (Saline water).…”

Synergic Effects of Salinity and Rhizoctonia solani (Kuhn) Infection on Growth and Yield Attributes of Rice (Oryza sativa L.)

“…Hence, this investigation endeavors to bridge this knowledge gap by examining the combined effects of salinity stress and R. solani infection on crucial growth parameters and yield components across diverse rice varieties. The findings will be instrumental in ensuring food security globally and agricultural sustainablity in the face of environmental challenges (Abu-Ria et al, 2023;Guigard et al, 2023;Taha et al, 2023).…”