The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction

Abstract

Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes. Methods: Thirty male Wistar rats were distributed into 3 groups of ten: spleen manipulation (SM); total splenectomy (TS); subtotal splenectomy with preservation of the inferior pole (IP). Rats were subjected to surgical procedure depending on the group. Seven days after surgery they underwent induction of peritonitis and survival time was recorded. All animals were subjected to two blood collections: before surgery and 70 days after it for TCD4/TCD8 lymphocyte counting. Results: Mean survival time was longer in the IP and SM groups and shorter in the TS group; there was significant difference between them. The comparison of the median number of CD4 did not present changes, whereas the comparison of the median number of CD8 decreased in the SM and IP groups. The correlation between the median number of TCD4 and TCD8 lymphocytes and the animals' survival was not significant. Conclusion: The maintenance of splenic tissue contributed to increase the survival of rats and there was a change in the number of TCD8 lymphocytes.

Study of the upper pole after subtotal splenectomy in rats

“…Borjaili et al . 28 compared the survival rates of rats submitted to SSPLP, total splenectomy, and spleen manipulation after induction of fecal peritonitis. The results showed that maintenance of the splenic tissue through SSPLP helped to increase the survival time of rats 28 .…”

“… 28 compared the survival rates of rats submitted to SSPLP, total splenectomy, and spleen manipulation after induction of fecal peritonitis. The results showed that maintenance of the splenic tissue through SSPLP helped to increase the survival time of rats 28 . This study reinforces the functionality of the conservative surgery with preservation of the LP, but further studies are necessary with the aim of proving that SSPUP is also able to guarantee increased survival rate in rats after inducing fecal peritonitis.…”