The Influence of Stabilizators on the Growth, Viability and Functional Activity of Bradyrhizobium Japonicum

Kozar, S. F.; Skorik, V.V; Usmanova, T. O.; Evtushenko, T.A.

doi:10.35868/1997-3004.15.58-70

Cited by 3 publications

(2 citation statements)

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“…To obtain a bacterial culture for inoculation, the bacteria were cultivated on a medium for slow-growing nodulating bacteria on pea broth with the following composition (g/L): sucrose -5.0 (Hlr LLC, China); glucose -10.0 (Hlr LLC, Ukraine); (NH 4 ) 2 SO 4 -1.0 (Merk, Germany); KH 2 PO 4 -0.5 (Hlr LLC, China); K 2 HPO 4 -0.5 (Hlr LLC, China); MgSO 4 •7H 2 O -0.2 (Merk, Germany); CaCO 3 -0.2 (Hlr LLC, Ukraine); NH 4 MoO 4 -0.05 (Merk, Germany); pea broth (100 g of peas boiled in 1 liter of tap water for 30-40 minutes), agar -12.0, pH = 6.8, sterilization regime -1 atm, 30 minutes (Kozar et al, 2012). For bacterial inoculation, a cell suspension was prepared by washing the bacterial culture with a NaCl solution (0.8%, Hlr LLC, Ukraine) on solid medium.…”

Section: Methodsmentioning

confidence: 99%

Influence of Bradyrhizobium japonicum on the growth parameters and formation of the assimilation apparatus in E-gene isogenic lines of soybean

Hlushach,

Avksentieva

2024

Regul. Mech. Biosyst.

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The study investigated the impact of the interaction between soybean and rhizobia on the assimilation apparatus functioning and biomass accumulation in different soybean lines with varying photoperiod sensitivity. Nearly isogenic lines (NILs) of soybean were used, with genes E1, E2, and E3 in different allelic states: Clark (e1E2E3), L80-5879 (E1e2e3), L63-3117 (e1e2E3), and L71-920 (e1e2e3). The experimental group for each line was treated with Bradyrhizobium japonicum 634b. Plants were grown under natural long-day conditions (16 hours). Growth indicators of the studied lines, such as relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), and specific leaf area (SLA), were analyzed, as well as the content of chlorophylls A and B in the V3 and V5 developmental stages. The results demonstrate that the influence of rhizobia on the functioning of the assimilation apparatus and biomass accumulation depends on the soybean line genotype. In the study, RGR, which characterizes the biomass accumulation rate, has similar trends to those observed with NAR, characterizing the assimilation apparatus's functioning. However, each line showed its own tendencies. For instance, in the short-day variety Clark, under bacterial influence, the value of RGR and NAR decreased. Additionally, LAR and SLA values indicated a reduction in the total photosynthetic surface area and leaf dry matter. Bacterial inoculation did not significantly affect the content of photosynthetic pigments in Clark leaves. Another short-day line, L80-5879, showed no significant impact of bacterial inoculation on biomass accumulation. However, soybean interaction with Bradyrhizobium japonicum 634b led to a decrease in leaf surface area and dry matter content. Probably, bacterial inoculation supported assimilation processes by increasing auxiliary chlorophyll b in photosystem I. A general trend of significant RGR reduction in neutral-day soybean lines, L63-3117 and L71-920, was identified under bacterial influence. The interaction with rhizobia differently affected LAR and SLA values, indicating distinct adaptive mechanisms to the interactions. In conditions of almost zero plant biomass accumulation, Bradyrhizobium japonicum 634b caused a decrease in the total photosynthetic surface area and chlorophyll a and b content in the L63-3117 line. In L71-920, bacterial inoculation had no effect on the total photosynthetic surface area, while leaf dry matter and photosynthetic pigment content decreased. The obtained results demonstrate that interaction with rhizobia can influence the functioning of the assimilation apparatus in soybeans with varying photoperiod sensitivity that is determined by genotype. It is important in improving soybean productivity and its application in agricultural practices.

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Section: Methodsmentioning

confidence: 99%

Influence of Bradyrhizobium japonicum on the growth parameters and formation of the assimilation apparatus in E-gene isogenic lines of soybean

Hlushach,

Avksentieva

2024

Regul. Mech. Biosyst.

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“…Водночас важливим критерієм оцінки нових біопрепаратів є економічна ефективність їхзастосування. Практично всі сільськогосподарські товаровиробники керуються саме цим у виборі нових інноваційних продуктів для підвищення врожайності та отримання якісної продукції [8] Для підвищення життєздатності бульбочкових бактерій застосовували полісахаридно-білковий комплекс (ПБК) [16]. Інокуляцію здійснювали шляхом передпосівної бактеризації насіння гороху з розрахунку 200 тис.…”

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Economic and Energy Efficiency of Pea Inoculation When Growing Crop in the System of Organic Production

Kozar,

Khalep,

Yevtushenko

et al. 2023

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Objective. To analyse economic and energy efficiency of inoculants based on a new strain Rhizobium leguminosarum Г222 in the technology of growing field peas. Methods. The calculation of the indicators of the economic efficiency of the cultivation of field peas for various seed treatment variants was carried out using methods and commonly used methodological approaches, which are based on a comparison of the results of the application of a certain agricultural measure with the costs of its implementation. The following indicators were studied: profit, cost per unit, payback of additional costs, as well as the level of profitability of production. Modelling of technological costs in the conditions of production scales using typical pea growing technologies has been used. The main agrotechnical operations have been modelled according to resource cost standards and product costing algorithms with the inclusion of additional operations and costs associated with the use of studied inoculants. In order to calculate the main indicators of the energy efficiency of the applied means for treatment of the pea seeds, the costs of all types of used resources and the obtained products were converted into energy equivalents according to generally accepted methods. Results. The use of inoculants based on R. leguminosarum Г222 contributes to increase in all the main indicators of economic efficiency: cost per unit, profit based on 1 ha of sown area and the level of profitability increase with the corresponding level of payback of additional costs. This is due to the higher rates of yield growth (and, accordingly, cash receipts) in experimental variants compared to the rates of increase of costs per unit area associated with the use of inoculants. According to the results of the energy efficiency analysis, it has been shown that the inoculation of peas with a new strain provides a high level of energy efficiency in terms of the ratio of the energy content of the obtained products to the energy consumption for its production (energy efficiency coefficient). The results of the comparative analysis of the calculated indicators show that in the variants with bacterization, there is a significant lag in the rates of increase of anthropogenic energy consumption per 1 ha of sown area versus the increase in yield and energy content of the obtained products. Additional energy costs associated with the use of inoculants are 8.00–24.39 times paid back by the energy of the additional harvest. Conclusion. The results of the analysis suggest high economic and energy efficiency of the use of the new strain R. leguminosarum Г222. The variant in which the use of R. leguminosarum Г222 was combined with a polysaccharide-protein complex is the most efficient.

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Influence of genotype and bacterization on growth, development, and soluble carbohydrate content in soybean E-genes isogenic lines

Hlushach,

Zhmurko,

Avksentieva

2023

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

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Photoperiod, which regulates the duration of vegetative and generative development, and the plant-microorganism interaction, which influences the metabolic status of plant organisms, are important factors in the regulating plant growth and development. The aim of the study was to determine the influence of Glycine max (L.) Merr. genotype and seed pre-bacterization with a virulent and active strain of Bradyrhizobium japonicum 634b on the plant growth and development, and on the soluble carbohydrate content in leaves of isogenic by E-genes lines under field conditions. Nearly isogenic lines (NILs) of soybean, in which the E1, E2, and E3 genes are located at different allelic loci, were used. Sterile seeds were pretreated with distilled water (control) and Bradyrhizobium japonicum 634b cell suspension (experiment). Plants were grown under natural long-day conditions (16 hours). The growth and development of the soybean were evaluated by phenological observations, morphometric indicators fixed at the V3 and V5 developmental stages, relative growth rate (RGR), and the content of soluble sugars ‒ mono- and oligosaccharides. The effect of the factors studied (genotype, bacterization, and their interaction) was calculated. The results of the experiment and the calculation of the effect of the factor showed that the isoline genotype has the greatest effect on seed germination, phenological development of the plant and duration of the VE-R1 phase, growth of the root system in the V3 and V5 phases, and the content of monosaccharides involved in forming the plant-microorganism interaction. The effect of bacterization is most evident in the RGR, shoot development, and the oligosaccharide content of the leaves of NILs in the V3 and V5 phases. Among the isolines studied, L 80-5879, which has the E1 gene (flowering repressor) in a dominant state, was characterized by minimal sensitivity to bacterization. It was found that bacterization and genotype interaction didn't influence the VE-R1 duration stage and the shoot and root length. The results obtained therefore prove that the E-series genes, which determine the photoperiodic sensitivity of soya beans, can also be indirectly involved in establishing plant-microorganism interactions.

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The Influence of Stabilizators on the Growth, Viability and Functional Activity of Bradyrhizobium Japonicum

Cited by 3 publications

References 1 publication

Influence of Bradyrhizobium japonicum on the growth parameters and formation of the assimilation apparatus in E-gene isogenic lines of soybean

Influence of Bradyrhizobium japonicum on the growth parameters and formation of the assimilation apparatus in E-gene isogenic lines of soybean

Economic and Energy Efficiency of Pea Inoculation When Growing Crop in the System of Organic Production

Influence of genotype and bacterization on growth, development, and soluble carbohydrate content in soybean E-genes isogenic lines

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