The influence of static magnetic fields on canine and equine mesenchymal stem cells derived from adipose tissue

Abstract

The aim of this study was to evaluate the proliferation rate and morphological changes of adipose-derived mesenchymal stem cells of canine and equine origin (Eq- and CaAdMSC). Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. Proliferation activity of cells was determined with the Alamar Blue assay. Obtained results, normalized in respect to the control culture, showed that EqAdMSC exposed to MF maintained a high proliferation status, whereas proliferation activity of CaAdMSC cultured in the presence of MF was decreased. Estimations of population doubling time (PDT) also revealed that EqAdMSCs exposed to static MF achieved a twofold increase in the total number of cells in a shorter amount of time than the control culture. The PDT value obtained for investigated CaAdMSCs indicated that MF exposure resulted in the prolongation of population doubling time. Morphology of cells and cellular composition was investigated using a light inverted microscope and a fluorescent microscope. A scanning electron microscope was used for microvesicles (MVs) imaging. Obtained results showed that both cell types maintained fibroblastic morphology and did not reveal signs of apoptosis or necrosis. However, the MF had an influence on the MVs secretion. While EqAdMSCs propagated in the presence of MF were characterized by the abundant MVs presence, CaAdMSCs revealed poor secretory activity. The approach presented provides complex analysis, which enables one to determine changes in equine and canine cytophysiology.

The effect of low static magnetic field on osteogenic and adipogenic differentiation potential of human adipose stromal/stem cells

“…The exposure system used in this study was previously described by Marędziak et al [25]. A pair of permanent neodymium magnets with a known magnetic polarization vector was used to expose the cells to SMF.…”

“…Thus, searching for factors able to enhance cell viability seems to be essential when application of MSCs in the field of regenerative medicine is considered. In our previous studies we have demonstrated that ASCs proliferation rate and their population doubling time (PDT) are distinctly increased when treated with a static magnetic field (SMF) [25]. It was previously shown that SMF might affect the behavior of cells [26].…”

The Autologous Gelsolin Combined with Exogenous Nucleotides enhance Chondrogenic Differentiation in Equine Adipose Derived Mesenchymal Stromal Cells - An In Vitro Research

“…Subcutaneous adipose tissue was collected from the tail base area, according to standard surgical procedure, using local anesthesia induced by 2% lignocaine (Polfa S.A., Poland) [35]. The total amount of collected tissue samples was equal 3 grams.…”

“…According to previous studies [35] the first 24 hr after isolation equine stem cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) with Nutrient F-12, whereas DMEM containing 4500 mg/L of glucose was applied for the secondary cell culture. The media were supplemented with 10% of fetal bovine serum (FBS), and 1% of antibiotic-antimycotic solution.…”

An in vitro study of the impact of 4mT static magnetic field to modify the differentiation rate of rat bone marrow stem cells into primordial germ cells

“…The interest in the variation biological effects of non-ionizing electromagnetic fields (EMFs) on the whole organism, as well as on cellular systems, has considerably increased in recent years in consideration of their probable health risk for humans (Pagliara et al, 2009). Several studies have shown that MF has an influence on a large variety of cellular functions; nevertheless the its exact mechanism(s) still is not clear (Marędziak et al, 2014). Few studies exist on the effects of static magnetic fields (SMFs) on living cells and tissues, compared with low-frequency magnetic fields.…”