The influence of storage temperature during machine perfusion on preservation quality of marginal donor livers

Olschewski, Peter; Gaß, Paul; Ariyakhagorn, Veeravorn; Jasse, Kerstin; Hunold, Gerhard; Menzel, Martin; Schöning, Wenzel; Schmitz, Volker; Neuhaus, P.; Puhl, Gero

doi:10.1016/j.cryobiol.2010.03.005

Cited by 80 publications

(48 citation statements)

References 28 publications

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“…In accordance with these considerations, no signs of anaerobiosis (HIF-1α) or liver injury were observed during oxygenated perfusion at 10 or 20 °C in contrast to perfusion at 30 or 37 °C [60]. Other rodent models demonstrated superior results at 21 °C compared to lower temperatures in DBD [61,62] and DCD [63] models. This indicates an important shift in hepatic metabolism between 20 and 30 °C, explaining the favorable ATP concentrations after subnormothermic machine perfusion (SNTM).…”

Section: Subnormothermic Machine Perfusionmentioning

confidence: 52%

Graft Reconditioning before Liver Transplantation

Hoyer

Minor

2016

Visc Med

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Background: The high demand for livers for transplantation has led to organs of limited quality being accepted to expand the donor pool. This is associated with inferior outcomes due to more pronounced preservation injury. Accordingly, recent research has aimed to develop preservation modalities for improved preservation as well as strategies for liver viability assessment and liver reconditioning. Methods: The PubMed database was searched using the terms ‘perfusion', ‘liver', ‘preservation', and ‘reconditioning' in various combinations, and the according literature was reviewed. Results: Several perfusion techniques have been developed in recent years with the potential for liver reconditioning. Preclinical and first emerging clinical data suggest feasibility, safety, and superiority over the current gold standard of cold storage. Conclusion: This review outlines current advances in the field of liver preservation with an emphasis on liver reconditioning methods.

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Section: Subnormothermic Machine Perfusionmentioning

confidence: 52%

Graft Reconditioning before Liver Transplantation

Hoyer

Minor

2016

Visc Med

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“…Three new solutions that are being tested are Vasosol [16], Polysol (Doorzand Medical Innovations, Amsterdam, The Netherlands) and Lifor an acellular oxygen carrying solution being developed by Life Blood Medical, Inc., which they claim has provided good in vitro function data employing a working heart model for 10 hour slow perfusion preservation of guinea pig hearts at room temperature without added oxygen [30]. More recently, a rat liver study with oxygenation was reported comparing several temperatures with the best results at room temprature [31]. We employed KPS-1 (Kidney Perfusion Solution One), a variant of University of Wisconsin solution commonly known as Belzer's Machine Perfusion Solution, oxygenated and supplemented as described in the materials and methods section.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo evaluation of porcine livers post-hypothermic machine perfusion at 4-6ºC and 12-14ºC

Brockbank¹,

Lee²,

Greene³

et al. 2012

J Regen Med Tissue Eng

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Background: The goal of our research is the development of a clinical hypothermic machine perfusion method and device for liver preservation during storage and transport prior to transplantation. The purpose of this study was comparison of two hypothermic temperature ranges. Methods: Heart beating donor pig livers with 2h of static cold storage on ice were employed. Thirteen experimental livers were perfused, at either 4-6ºC or 12-14ºC with oxygenation, employing a prototype device. They were compared with six control 2h static cold stored livers during normothermic ex vivo blood perfusion. Physiological measurements and biochemical perfusate analyses were performed to assess the impact of storage conditions on liver functions. Statistics were assessed by one way analysis of variance, p<0.05 was regarded as significant. Results: During hypothermic perfusion perfusate solutes (Na

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“…Given that the liver is fully metabolically active, NMP also offers the best opportunity to assess and even improve graft viability prior to reperfusion in vivo (52,57). NMP may lead to sustained viability, improved hemodynamics and attenuate ischemic injury in marginal livers (58). Nevertheless, the high concentration of pro-inflammatory cytokines in the perfusate during NMP after a prolonged period of CS promote concerns on the capacity of NMP to generate effective graft reconditioning and excellent cell metabolism (59) Friend et al were amongst the first to describe NMP and its benefits over standard CS as measured by preservation of alanine-aminotransferase (ALT) and enhanced Factor V production (60).…”

Section: Nmpmentioning

confidence: 99%

Perfusion machines and hepatocellular carcinoma: a good match between a marginal organ and an advanced disease?

Ghinolfi

Rreka

Pezzati

et al. 2017

Transl. Gastroenterol. Hepatol.

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Hepatocellular carcinoma (HCC) accounts for 90% of primary liver cancers, is the second leading cause of cancer-related deaths and the leading cause of death in patients with cirrhosis. Liver transplantation (LT) represents the ideal treatment for selected patients as it removes both the tumor and the underlying cirrhotic liver with 5-year survival rates higher than 70%. Unfortunately, due to tumor characteristics, patient co-morbidities or shortage of organs available for transplant, only 20% of patients can undergo curative treatment. Ex situ machine perfusion (MP) is a technology recently introduced that might potentially improve organ preservation, allow graft assessment and increase the pool of available organs. The purpose of this review is to provide an update on the current role of ex situ liver MP in liver transplantation for HCC patients.

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The influence of storage temperature during machine perfusion on preservation quality of marginal donor livers

Cited by 80 publications

References 28 publications

Graft Reconditioning before Liver Transplantation

Graft Reconditioning before Liver Transplantation

Ex vivo evaluation of porcine livers post-hypothermic machine perfusion at 4-6ºC and 12-14ºC

Perfusion machines and hepatocellular carcinoma: a good match between a marginal organ and an advanced disease?

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