The influence of surface roughness and surface dynamics on the attachment of Methicillin-Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; onto orthodontic retainer materials

Groosh, Dheaa H Al; Bozec, Laurent; Pratten, Jonathan; Hunt, NP

doi:10.4012/dmj.2014-045

Cited by 17 publications

(20 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that roughness of a surface increases the area of contact and provides protective recesses for the bacteria against the forces of displacement (Al Groosh et al . ).…”

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing

Nagay

Goiato

Silva

et al. 2018

Lett Appl Microbiol

View full text Add to dashboard Cite

Conditions of the acrylic resin (AR) surface, such as roughness, can promote a favourable environment for the adhesion of micro‐organisms, even on the surface of ocular prostheses. This study evaluated the influence of photopolymerized glaze application on the roughness of ARs and adhesion of Staphylococcus aureus and Staphylococcus epidermidis on ocular AR surfaces submitted to accelerated ageing. Two hundred and eighty‐eight samples of white colour (N1) and colourless ARs were distributed in eight groups (n = 9), based on surface treatments (glaze or ARs submitted to only a final polishing), accelerated ageing (before and after) and periods of microbial growth (24‐ and 48‐h). The roughness average (Ra) and total height of roughness profile (Rt) values were greater for the groups with glaze and increased for all groups after ageing. The microbial adhesion among the groups with and without glaze did not present a statistically significant difference. The ageing did not statistically affect the adhesion of Staph. epidermidis, but affected the adhesion of Staph. aureus, which presented an increase after 24 h of growth on only N1 AR with glaze. These results demonstrate that the glaze did not contribute to adhesion of Staph. aureus and Staph. epidermidis, which are responsible for most ocular prosthetic infections. Significance and Impact of the Study Some recent evidence suggested that the surface finish of ocular prostheses influences the accumulation of deposits that can affect the interaction with pathogenic bacteria, increasing the probability of infections. In addition, surface deterioration over time can increase the roughness and, consequently, biofilm formation. Thus, a better understanding of the influence of surface finish on bacterial adhesion becomes extremely important. In this study, we tested a glaze for surface polishing compared to mechanical polishing, before and after ageing. The results suggest that the glaze did not contribute to microbial adhesion and might be useful in preventing possible prosthetic infections.

show abstract

“…It is known that roughness of a surface increases the area of contact and provides protective recesses for the bacteria against the forces of displacement (Al Groosh et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…; Al Groosh et al . ). In this study, the minimum Ra value was 0·038 μ m (Table ), encountered for N1 AR without glaze before accelerated ageing ( Staph.…”

Section: Resultsmentioning

confidence: 97%

Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing

Nagay

Goiato

Silva

et al. 2018

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…The lack of association between surface roughness and microbial adhesion as found in this study suggests that surface roughness may not be the predominant factor for microbial adherence on VFRs. Other surface characteristics such as surface attraction to water and surface free energy also contribute to various biochemical interactions that influence the initial adhesion of microorganisms to surfaces [ 28 ]. Thermoplastic VFRs have smoother and more hydrophilic surfaces, higher electron donor properties, and acid-base interactions compared to auto-polymerized acrylic resins, commonly used for Hawley retainers.…”

Section: Discussionmentioning

confidence: 99%

Microbial adherence on vacuum-formed retainers with different surface roughness as constructed from conventional stone models and 3D printed models: a randomized controlled clinical trial

et al. 2023

View full text Add to dashboard Cite

Objective This single center parallel, randomized controlled trial aimed to determine the propensity of microbial adherence on vacuum-formed retainers (VFRs) with different surface roughness imprints. Materials and methods Thirty-six patients debonded from fixed appliances at a teaching institution were allocated by block randomization stratified for gender to three groups [VFRs fabricated on conventional, fused deposition modeling (FDM) or stereolithography apparatus (SLA) working models]. Participants wore the VFRs for three months full-time followed by three months part-time. VFRs were collected after each follow-up for Streptococcus and yeast counts. Surface roughness was measured indirectly on the working models using a 3D optical surface texture analyzer. Blinding was not feasible due to appliance appearance. The trial was registered [NCT03844425 ( ClinicalTrials.gov )] and funded by the Universiti Malaya Dental Postgraduate Research Grant (DPRG/14/19). Results Thirty participants (eleven conventional, ten FDM, and nine SLA) were analyzed after six dropped out. No harms were reported. Microbial counts between the groups were not significantly different. There were more microbes in the lower VFRs than upper VFRs (total count: p <0.05; effect size, 0.5 during full-time wear and 0.4 during part-time wear). SLA had significantly ( p <0.05) smoother surface than FDM (effect size, 0.3) and conventional models (effect size, 0.5). Microbial adherence was not associated with working model surface roughness. Conclusion Microbial adherence on VFRs was not influenced by degree of surface roughness imprints from working models. Clinical relevance 3D printed models can be used to make VFRs. Lower VFRs tended to accumulate oral microbes, potentially increasing the oral health risk in the lower arch.

show abstract

“…PTFE demonstrated the highest maximum surface feature width and depth measurements and provided evidence that this surface could trap and retain the E. coli in the surface features which were a similar size to the dimensions of bacterial cells [ 6 , 34 ]. This could result in a reduction of bacterial cells recovered by conferring protection from shearing forces [ 47 , 48 ]. The PTFE surface achieved the lowest percentage of surface coverage of E. coli cells before the cleaning of the surfaces.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Surface Topography and Wettability on Escherichia coli Removal from Polymeric Materials in the Presence of a Blood Conditioning Film

Akhidime

Slate

Hulme

et al. 2020

IJERPH

View full text Add to dashboard Cite

The reduction of biofouling and the reduction of cross-contamination in the food industry are important aspects of safety management systems. Polymeric surfaces are used extensively throughout the food production industry and therefore ensuring that effective cleaning regimes are conducted is vital. Throughout this study, the influence of the surface characteristics of three different polymeric surfaces, polytetrafluoroethylene (PTFE), poly(methyl methacrylate) (PMMA) and polyethylene terephthalate (PET), on the removal of Escherichia coli using a wipe clean method utilising 3% sodium hypochlorite was determined. The PTFE surfaces were the roughest and demonstrated the least wettable surface (118.8°), followed by the PMMA (75.2°) and PET surfaces (53.9°). Following cleaning with a 3% sodium hypochlorite solution, bacteria were completely removed from the PTFE surfaces, whilst the PMMA and PET surfaces still had high numbers of bacteria recovered (1.2 × 107 CFU/mL and 6.3 × 107 CFU/mL, respectively). When bacterial suspensions were applied to the surfaces in the presence of a blood conditioning film, cleaning with sodium hypochlorite demonstrated that no bacteria were recovered from the PMMA surface. However, on both the PTFE and PET surfaces, bacteria were recovered at lower concentrations (2.0 × 102 CFU/mL and 1.3 × 103 CFU/mL, respectively). ATP bioluminescence results demonstrated significantly different ATP concentrations on the surfaces when soiled (PTFE: 132 relative light units (RLU), PMMA: 80 RLU and PET: 99 RLU). Following cleaning, both in the presence and absence of a blood conditioning film, all the surfaces were considered clean, producing ATP concentrations in the range of 0–2 RLU. The results generated in this study demonstrated that the presence of a blood conditioning film significantly altered the removal of bacteria from the polymeric surfaces following a standard cleaning regime. Conditioning films which represent the environment where the surface is intended to be used should be a vital part of the test regime to ensure an effective disinfection process.

show abstract

The influence of surface roughness and surface dynamics on the attachment of Methicillin-Resistant <i>Staphylococcus aureus</i> onto orthodontic retainer materials

Cited by 17 publications

References 43 publications

Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing

Effect of photopolymerized glaze application on bacterial adhesion on ocular acrylic resin surfaces submitted to accelerated ageing

Microbial adherence on vacuum-formed retainers with different surface roughness as constructed from conventional stone models and 3D printed models: a randomized controlled clinical trial

The Influence of Surface Topography and Wettability on Escherichia coli Removal from Polymeric Materials in the Presence of a Blood Conditioning Film

Contact Info

Product

Resources

About