The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

Abstract

Lallemantia iberica (Lamiaceae) has pharmaceutical and nutritional importance owing to production of oily seeds and synthesis of commercially secondary metabolites. In the present study, total phenol and flavonoids content and quantitative changes in the shoot and callus pigments of L. iberica were investigated. Moreover, some growth parameters and volatile content of in vitro grown shoots were analyzed. Nodal segments and epicotyldons were used as the explants for shoot and callus formation, respectively. In the multiplication stage of shoot culture, MS medium supplemented with six concentrations of TDZ was employed. For callus induction, 1 mg/l of TDZ, NAA and a combination of NAA and BA were used. Extracted volatile products were analyzed by GC-mass spectrometry. According to the results, the concentration range of TDZ from 0.22 to 0.66 mg/l improved the flavonoids production and the highest content was reported to be in 2 mg/l TDZ (157.5 ± 25.9 mg QE/ g); also, the highest amount of total phenol (3.86 ± 1.02 mg PYRO/g) was observed in 0.66 mg/l TDZ. For induced calli, among the three tested types of PGRs, TDZ treatment was efficient for total phenol and flavonoids. In general, the higher content of flavonoids was obtained from L. iberica shoots while the callus of this plant was efficient for total phenol production. 55 components were identified using GC-MS analysis indicating a low amount of mono and sesquiterpenes and high quantity of alkan hydrocarbons.

Melatonin-enhanced biosynthesis of antimicrobial AgNPs by improving the phytochemical reducing potential of a callus culture of Ocimum basilicum L. var. thyrsiflora

“…The maximum production of TPC is well reported in in vitro cultures in response to TDZ. 39 In our experiments, various phytohormones (TDZ, 2,4-D, and NAA) and the explant used were found to be responsible for the TPC in the callus cultures (Fig. 3a).…”

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

“…The TCC was significantly decreased in leaves of in vitro-regenerated shoots cultured on MS medium with 2.0 µM BA or TDZ. The inhibitory effect of cytokinin on TCC has been reported in banana (Aremu et al 2012 ) and Lallemantia iberica (Pourebad et al 2015 ).…”

A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

“…For some of the endangered or high medicinal value species, the clonal propagation is essential (Filipović et al 2015). Shoots, leaves, nodal segments and less frequently, roots are used as explants to promote organogenesis phenomena (Mallón et al 2011;Pourebad et al 2015). In our assay, shoot regeneration was successfully induced from the above five types of explants of S. nigrum at a high frequency.…”