In this paper, the shear-thickening fluids(STFs) are prepared by 0.5 µm and 12 nm silica particles. The STFs rheological properties and the performance of stab resistance are studied. SiO2 particles with two sizes (12 nm and 0.5 µm) are mixed at different weight ratios to form shear thickening fluids and its rheological behaviors are tested. Next, the quasi-static spike and knife stab resistances, dynamic impact strength, and yarn pull-out of shear thickening fluid-impregnated Kevlar fabrics are tested. The investigation showed that the micro- and nano-sized particles of silica are present in the compound shear thickening fluid, and the rheological behavior was positively improved. Regardless of whether shear thickening fluid is composed of mono-sized or binary particle-sized SiO2 particles, the presence of shear thickening fluids can significantly strengthen Kevlar fabrics in terms of the quasi-static spike and knife stab resistances, dynamic impact strength, and promote the friction force of inter-yarn. In particular, shear thickening fluid-impregnated Kevlar fabrics have better performances when being composed of 12 nm and 0.5 µm SiO2 particles at ratios of 2:1, 1:1, and 1:2 than when being made of only micro-sized or nano-sized particles.