The influence of temperature on red cell deformability

Williamson, J R; Shanahan, MO; Hochmuth, Robert M.

doi:10.1182/blood.v46.4.611.bloodjournal464611

Cited by 22 publications

(30 citation statements)

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“…Surface roughness and surface chemistry influence hemolysis and may even dominate in capillary flow, especially at shear stress below the critical limit of $150 Pa. Erthroyctes attach to surfaces and develop tethers if exposed to shear stress above 0.2 Pa (36). Hemolysis correlates with the critical surface energy with the exception of PTFE (Teflon) which, although having a low critical surface energy, causes the most hemolysis (37).…”

Section: Surface Effectsmentioning

confidence: 99%

Red Blood Cell Damage from Extracorporeal Circulation in Hemodialysis

Polaschegg¹

2009

Seminars in Dialysis

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Blood damage is an unavoidable side effect of extracorporeal circulation. The effects of blood damage on patients' hematocrit and erythropoietin requirement as well as other potential side effects have not been studied for uneventful treatments. Comparing long nocturnal dialysis with regular 4-hour, three times per week dialysis allows for the conclusion that the influence of blood damage caused by extracorporeal circulation is small compared with biochemical effects. Acute hemolysis is one of the few remaining mechanical problems of dialysis. Acute hemolysis is caused by obstructions within the extracorporeal circuit caused by manufacturing errors, kinking of blood tubing or user errors, or by a combination of excessive flow and improper cannula or catheter dimensions. The risk of acute hemolysis can be further reduced by industrial quality control, better design of dialysis equipment, and hemodialysis machine control. Adverse effects caused by chronic mechanical hemolysis need to be studied.

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Section: Surface Effectsmentioning

confidence: 99%

Red Blood Cell Damage from Extracorporeal Circulation in Hemodialysis

Polaschegg¹

2009

Seminars in Dialysis

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“…Our present goal was to evaluate a potential contribution of red cell deformability change to the increase in skin blood flow induced by local heating. Increasing temperature has been shown to increase membrane deformability of erythrocytes (Williamson et al, 1975). This same phenomenon may make it easier for red cells to pass through capillaries.…”

Section: Introductionmentioning

confidence: 97%

The effect of increasing temperature on skin blood flow and red cell deformability

Rendell

Kelly

Bamisedun

et al. 1993

Clinical Physiology

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Using laser Doppler techniques in nine healthy volunteers, we contrasted the effect of increasing local skin temperature at the elbow, a skin site with nutritive microvasculature, and the finger pulp, with predominantly arteriovenous anastomic (AVA) perfusion). We also assessed flow at the finger dorsum, with contributions of both types of microvasculature. In parallel with the laser Doppler studies, we determined the effect of increasing temperature on the red cell deformability of our subjects, using the new technique of Cell Transit Time Analysis (CTTA). Thermal stimulation produced very large increases in skin blood flow at all three sites tested. However, the magnitude and the pattern of increase were different at the three sites. At the finger pulp, there was a linear approximately threefold increase in flow as temperature increased from the basal level to 44 degrees C. At the elbow, basal flow was considerably lower than at the finger pulp and increased very slowly until skin temperature reached 38 degrees C. From that point, flow increased sharply, reaching tenfold the basal level at 44 degrees C. The thermally induced increase at the finger dorsum was intermediate between the other two sites, with a pattern resembling the elbow more than the finger pulp. These differences among the sites were attributable to substantially different patterns of change in the two components of flow, microvascular volume and velocity. At the finger pulp, there was very little increase in microvascular volume with increasing temperature. The curve was practically flat from basal temperature to 44 degrees C. In contrast, there was a linear increase in red blood cell velocity of about 300%. At the elbow, both microvascular volume and red blood cell velocity exhibited a parallel curvilinear pattern of equivalent increase, on the order of 300% for each. There was only a small increase in both parameters until the temperature reached 38 degrees, at which point there was a sharp increase in both. At the finger dorsum, the situation was intermediate, again resembling the elbow more than the finger pulp. Cell Transit Time Analysis revealed a progressive decrease in red cell transit time (TT), from 3.28 ms at 28 degrees C to 2.48 m at 44 degrees C, an overall change of 24%. The decrease in TT was accompanied by an increase in transit frequency, measured as counts s-1 (C s-1), from 3.1 to 5.3, an overall change of 71%. The changes in both TT and C/S were essentially linear.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…a value for Young's modulus or viscosity is valid only for a given temperature. Surprisingly, however, during the last 50 years only a few studies have focused on how cell rheology is affected by temperature [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Thermorheology of living cells—impact of temperature variations on cell mechanics

et al. 2013

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Upon temperature changes, we observe a systematic shift of creep compliance curves J (t) for single living breast epithelial cells. We use a dual-beam laser trap (optical stretcher) to induce temperature jumps within milliseconds, while simultaneously measuring the mechanical response of whole cells to optical force. The cellular mechanical response was found to differ between sudden temperature changes compared to slow, long-term changes implying adaptation of cytoskeletal structure. Interpreting optically induced cell deformation as a thermorheological experiment allows us to consistently explain data on the basis of time-temperature superposition, well known from classical polymer physics. Measured time shift factors give access to the activation energy of the viscous flow of MCF-10A breast cells, which was determined to be ≈ 80 kJ mol −1 . The presented measurements highlight the fundamental role that temperature plays for the deformability of cellular matter. We propose thermorheology as a powerful concept to assess the inherent material properties of living cells and to investigate cell regulatory responses upon environmental changes.

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The influence of temperature on red cell deformability

Cited by 22 publications

References 0 publications

Red Blood Cell Damage from Extracorporeal Circulation in Hemodialysis

Red Blood Cell Damage from Extracorporeal Circulation in Hemodialysis

The effect of increasing temperature on skin blood flow and red cell deformability

Thermorheology of living cells—impact of temperature variations on cell mechanics

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