The influence of testing angle on the biomechanical properties of the rat supraspinatus tendon

Newton, Michael D.; Davidson, Abigail A.; Pomajzl, Ryan J.; Seta, Joseph; Kurdziel, Michael D.; Maerz, Tristan

doi:10.1016/j.jbiomech.2016.11.003

Cited by 16 publications

(15 citation statements)

References 30 publications

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“…The power analysis was performed based on the previous study of Newton et al, who demonstrated that the mechanical properties of the suparpinatus tendon consisting of stress‐relaxation and load‐to‐failure were significantly affected by the abduction/testing angle. Using the G*Power 3.0 program, we calculated required sample size for the comparison between two independent means with the 1:1 allocation ratio, and the power analysis determined that 13 specimens were needed per group to detect a significant difference in ultimate failure load (comparison between two groups, mean difference = 35 N, standard deviation = 25 N, α error = 0.05, β error = 0.1, drop‐out rate = 20%) based on the previous study . The right shoulder of each rat was used for the biomechanical evaluation including assessment of ultimate failure load, while the left shoulder was used for histological analysis.…”

Section: Methodsmentioning

confidence: 99%

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Yoon

Chung

Jung

et al. 2019

Journal Orthopaedic Research

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To evaluate the effect of local parathyroid hormone (PTH) administration on rotator cuff tendon‐to‐bone healing in a rat model compared with systemic PTH injection and untreated controls. PTH‐alginate scaffold was prepared and sustained release of PTH was confirmed. Bilateral supraspinatus tendon repairs were performed in 39 rats (group 1, supraspinatus repair only; group 2, supraspinatus repair with systemic PTH injection; group 3, supraspinatus repair with local PTH administration via an absorbable scaffold; n = 13 each). Biomechanical (cross‐sectional area, mode of failure, load to failure, and ultimate stress: right side) and histological analyses (hematoxylin and eosin stain, Masson's Trichrome stain Picrosirius red stain, Immunohistochemistry for BMP2, PTH1R, ColI, and ColIII: Left side) were performed to evaluate tendon‐to‐bone healing quality at 8 weeks after repair, and blood test (osteocalcin and procollagen type I N‐terminal pro‐peptide [PINP] levels) was performed in all rats. There was no intergroup difference in the healing failure rate (p = 0.910) or failure mode (p = 0.585). Biomechanically, subjects in groups 2 and 3 exhibited significantly larger cross‐sectional areas and higher ultimate failure loads and ultimate stress than those in group 1 (all p < 0.05); however, no differences were noted between groups 2 and 3 (all p > 0.05). Histologically, groups 2 and 3 exhibited more organized tendon‐to‐bone interface structures with higher density, parallel orientation, and collagen fiber continuity than group 1 (all p < 0.05 except collagen fiber continuity in group 1 vs. 2); however, no differences in histological parameters between groups 2 and 3 (all p > 0.05). The protein levels of bone morphogenic protein 2, PTH 1 receptor, and collagen I and III and the serum level of PINP were increased in groups 2 and 3 versus group 1 (all p < 0.05) without showing differences between groups 2 and 3 (all p > 0.05). Local PTH administration using an absorbable scaffold improved the biomechanical and histological outcomes of rotator cuff tendon‐to‐bone healing comparable with systemic PTH injection at 8 weeks after repair in a rat model. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:82–91, 2020

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Section: Methodsmentioning

confidence: 99%

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Yoon

Chung

Jung

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…The main endpoint was the ultimate failure load at the 8th week postrepair. The power analysis was performed based on the previous study of Newton et al 21 Using the SPSS 19.0 software (Chicago), we calculated required sample size for the comparison between two independent means with the 1:1 allocation ratio, and the power analysis Animals (n = 120) were randomly assigned to six groups (n = 20 per group): group 1, the control group without operation; group 2, the TGF-β1 group receiving lentiviral vectors containing siRNAs against TGF-β1; group 3, the Smad2 group receiving lentiviral vectors containing siRNAs against Smad2; group 4, the Smad3 group receiving lentiviral vectors containing siRNAs against Smad3; group 5, the vector group receiving empty vector after surgery; group 6, the normal saline (NS) group receiving a subacromial injection of NS after surgery (the injection method mentioned was previously reported in our team 22 ). The rats were randomized using a computer-generated block randomization sequence (www.randomizer.org) before surgical repair.…”

Section: Experimental Groupmentioning

confidence: 99%

Inhibition of Smad3 promotes the healing of rotator cuff injury in a rat model

Wang

Zhou

Liu

et al. 2020

Journal Orthopaedic Research

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To investigate the effect of inhibiting transforming growth factor-β (TGF-β1)/ Smad2/3 signaling on rotator cuff (RC) healing. A bilateral supraspinatus tendon detachment-repair model of Sprague-Dawley (SD) rats was utilized. A total of 120 SD rats were randomly assigned to six groups and each group received the subacromial injection of normal saline, empty vectors, or lentiviral vectors containing small interfering RNA against TGF-β1, Smad2, Smad3 at the bone-tendon junction. Biomechanical and histological analyses were performed to evaluate bone-tendon junction healing quality at 8 weeks after repair. Histologically, scar healing was found in all surgical groups. Animals with inhibited Smad3 exhibited better bonetendon junction structures with higher density, parallel orientation, and collagen fiber continuity than other surgical group animals. Immunohistochemistry revealed that the protein expression level of collagen I in animals with inhibited Smad3 was more prominent compared with all other surgical groups. Biomechanically, Animals with inhibited Smad3 showed better results in the maximum load at 4, 6, and 8 weeks after surgery compared with other surgical groups. Besides, C3H10T1/2 (Smad3−) cells increased TT-D6 cell migration and tendon-associated genes expression (scleraxis, tenascin C, collagen I) in coculture system. We conclude that inhibition of Smad3 promotes RC tendon healing in the rat supraspinatus model.

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“…The humerus was pneumatically fixed at a 0° inclination in a Zwick BZ 2.5/TN1S uniaxial testing machine (ZwickRoell). 37 All specimens were preconditioned by 5 strain-increase cycles of 0.2% per second from 0.2 to 0.6 N until a maximum of 5% was reached. Maximum load to failure was defined as an outcome parameter by a strain increase of 0.5 mm/min.…”

Section: Methodsmentioning

confidence: 99%

Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model

et al. 2020

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Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study Design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro–computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head ( P = .005) and the medial humeral head ( P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head ( P = .004) and the medial humeral head ( P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group ( P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group ( r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical Relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations.

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The influence of testing angle on the biomechanical properties of the rat supraspinatus tendon

Cited by 16 publications

References 30 publications

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Inhibition of Smad3 promotes the healing of rotator cuff injury in a rat model

Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model

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