The influence of the aphid-specific pathogen<i>Conidiobolus obscurus</i>(Entomophthoromycota: Entomophthorales) on the mortality and fecundity of bamboo aphids

Zhou, Xiang; Wang, Dawei; Zhang, Xin; Wang, Jianghong

doi:10.1007/s10310-013-0431-0

Cited by 11 publications

(9 citation statements)

References 22 publications

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“…The virulence of the mycelial mats that were generated by the 1 st , 4 th , and 8 th subcultures were assessed based on multi-concentration bioassays. The test bamboo aphids ( Takecallis taiwanus ) were reared in potted bamboo plants Chimonobambusa quadrangularis (Fenzi) Makino, at 24 ± 1°C in a light:dark 12 h:12 h [ 30 ]. Before the bioassay, 10 adults (alatae of T. taiwanus ) were allowed to freely produce progenies on each bamboo leaf-inclusive dish.…”

Section: Methodsmentioning

confidence: 99%

Long noncoding RNAs are potentially involved in the degeneration of virulence in an aphid-obligate pathogen, Conidiobolus obscurus (Entomophthoromycotina)

Zhang

Zhou

2021

Virulence

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Virulence attenuation frequently occurs in in vitro culturing of pathogenic microbes. In this study, we investigated the total putative long noncoding RNAs (lncRNAs) in an aphid-obligate pathogen, Conidiobolus obscurus , and screened the differentially expressed (DE) lncRNAs and protein-coding genes involved in the virulence decline. The virulence was significantly attenuated after eight subculturing events, in which the median lethal concentration of the conidia ejected from mycelial mats relative to the bamboo aphid, Takecallis taiwanus , increased from 36.1 to 126.1 conidia mm –2 , four days after inoculation. In total, 1,252 lncRNAs were identified based on the genome-wide transcriptional analysis. By characterizing their molecular structures and expression patterns, we found that the lncRNAs possessed shorter transcripts, lower expression, and fewer exons than did protein-coding genes in C. obscurus . A total of 410 DE genes of 329 protein-coding genes and 81 lncRNAs were identified. The functional enrichment analysis showed the DE genes were enriched in peptidase activity, protein folding, autophagy, and metabolism. Moreover, target prediction analysis of the 81 lncRNAs revealed 3,111 cis -regulated and 23 trans -regulated mRNAs, while 121 DE lncRNA-mRNA pairs were possibly involved in virulence decline. Moreover, the DE lncRNA-regulated target genes mainly encoded small heat shock proteins, secretory proteins, transporters, autophagy proteins, and other stress response-related proteins. This implies that the decline in virulence regulated by lncRNAs was likely associated with the environmental stress response of C. obscurus . Hence, these findings can provide insights into the lncRNA molecules of Entomophthoromycotina, with regards to virulence regulators of entomopathogens.

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Section: Methodsmentioning

confidence: 99%

Long noncoding RNAs are potentially involved in the degeneration of virulence in an aphid-obligate pathogen, Conidiobolus obscurus (Entomophthoromycotina)

Zhang

Zhou

2021

Virulence

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“…Thus, the Cyt function is probably associated with the time span from host infection to death. We observed that C. obscurus killed bamboo aphids within 24 h post infection, a phenomenon that might be attributable to the action of Cyt toxins . We also observed that Pieris rapae larvae feeding on cabbage leaves exposed to a conidial shower of C. obscurus displayed apocleisis behaviour and symptoms of paralysis (data not shown).…”

Section: Discussionmentioning

confidence: 63%

Transcriptomic insight into pathogenicity‐associated factors ofConidiobolus obscurus, an obligate aphid‐pathogenic fungus belonging to Entomopthoromycota

Wang¹,

Zhou

Guo³

et al. 2018

Pest Management Science

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“…Por otra parte, Witolda, determinó que la capacidad biocontroladora de Conidiobolus coronatus se debe a un complejo de enzimas lipolíticas y quitinolíticas capaces de degradar la cutícula de los insectos y provoca parálisis en este. En el presente estudio la cepa C11 (Conidiobolus sp) mostró la más alta actividad quitinolítica (véase en la gráfica 2), lo cual demuestra que Conidiobolus sp posee un alto potencial para degradar la quitina, sin embargo en el estudio su capacidad antagónica no fue representativa (35)(36)(37)(38)(39).…”

Section: Antagonismounclassified

Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani

Valderrama

2017

nova

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45Campuzano et al. Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani Evaluation of the cellulolytic and chitinolytic activity of rhizosphere isolated filamentous fungi from potato cultures to control rhizoctonia solani ResumenObjetivo. Evaluar la actividad celulolítica y quitinolítica de los hongos aislados de rizósfera de papa con posibilidades de ser usados para el control biológico de R. solani. Método. Se aislaron y clasificaron los hongos, mediante sus características macroscópicas y microscópicas. Se aplicaron pruebas para evaluar la capacidad celulolítica y quitinolítica de los hongos y se probó su capacidad antagónica frente al fitopatógeno. Se realizó análisis fisicoquímico del suelo del cual se aislaron los hongos de estudio. Resultado. Del total de hongos caracterizados, solamente una cepa de Rhizopus sp determinada como TN8 fue la que presentó la mejor actividad antagónica frente a R. solani, lo cual, permite considerar este hongo como posible agente de biocontrol para el fitopatógeno de estudio. Palabras claves:Control biológico, hongos celulíticos, hongos quitinolíticos, papa, plaga. AbstractObjective. To evaluate the cellulolytic and chitinolytic activity of fungi isolated from potato rhizosphere with possibilities of being used for the biological control of R. solani. Method. The fungi were isolated and classified by their macroscopic and microscopic characteristics. Tests were applied to evaluate the cellulolytic and chitinolytic capabilities of fungi and their antagonistic capabilities against the phytopathogen were tested. Physicochemical analysis of the soil from which the fungi were isolated were also performed. Result. From the total characterized fungi, only one strain of Rhizopus sp was determined as TN8 and this was the only one that presented the best antagonistic activity against R. solani. This result lead us to consider that this fungus is a possible biocontrol agent for the phytopathogen of this study.

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The influence of the aphid-specific pathogenConidiobolus obscurus(Entomophthoromycota: Entomophthorales) on the mortality and fecundity of bamboo aphids

Cited by 11 publications

References 22 publications

Long noncoding RNAs are potentially involved in the degeneration of virulence in an aphid-obligate pathogen, Conidiobolus obscurus (Entomophthoromycotina)

Long noncoding RNAs are potentially involved in the degeneration of virulence in an aphid-obligate pathogen, Conidiobolus obscurus (Entomophthoromycotina)

Transcriptomic insight into pathogenicity‐associated factors ofConidiobolus obscurus, an obligate aphid‐pathogenic fungus belonging to Entomopthoromycota

Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani

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