The Influence of the Solid/Liquid Ratio on the Point of Zero Charge of Alumina

Todorović, Žaklina N.; Milonjić, Slobodan K.; Dondur, V.

doi:10.4028/www.scientific.net/msf.453-454.361

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…32 IgM has a neutral charge at its isoelectric point of pH 8.0, 33 while surface charge of alumina is neutral at pH 7.2. 34 Thus, the most favorable electrostatic interaction between IgM and alumina surfaces at the channel walls occurs between pH 7.2 and 8.0 which can cause the highest amount of IgM loading within the membrane (Figure 4C). In Figure 4D, we notice that the signal response of the biosensor prepared in pH 7.6 solution is most sensitive (steepest slope) toward WNV-DIII.…”

Section: Resultsmentioning

confidence: 99%

“…Hydrophobic interaction between protein and alumina at the protein isoelectric point is likely insignificant since the alumina surface is hydrophilic . IgM has a neutral charge at its isoelectric point of pH 8.0, while surface charge of alumina is neutral at pH 7.2 . Thus, the most favorable electrostatic interaction between IgM and alumina surfaces at the channel walls occurs between pH 7.2 and 8.0 which can cause the highest amount of IgM loading within the membrane (Figure C).…”

Section: Resultsmentioning

confidence: 99%

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Membrane-Based Electrochemical Nanobiosensor for the Detection of Virus

et al. 2009

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A membrane-based electrochemical nanobiosensor sensitive toward whole viral particles is fabricated by forming a submicrometer thick nanoporous alumina membrane over a platinum disk electrode. Antibody probe molecules are physically adsorbed onto the walls of the membrane nanochannels. The sensing signal is based on the monitoring of the electrode's Faradaic current response toward ferrocenemethanol, which is extremely sensitive to the formation of immunocomplex within the nanoporous membrane. This nanobiosensor is demonstrated for the sensing of West Nile virus protein domain III (WNV-DIII) and the inactivated West Nile viral particle, using anti-WNV-DIII immunoglobulin M (IgM) as the biorecognition probe. The detection of the viral protein and the particle are logarithmically linear up to 53 pg mL(-1) (R(2) = 0.99) and 50 viral particles per 100 mL (R(2) = 0.93) in pH 7, with extremely low detection limits of 4 pg mL(-1) and ca. 2 viral particles per 100 mL, comparable to sensitivities of polymerase chain reaction techniques. The relative standard deviation (RSD) of whole viral particle detection in whole blood serum is 6.9%. In addition, the simple nanobiosensor construction procedure, minimal sample preparation, and short detection time of 30 min are highly attractive properties and demonstrate that the detection of a wide range of proteins and viruses can be achieved.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Membrane-Based Electrochemical Nanobiosensor for the Detection of Virus

et al. 2009

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“…Antibody loading increases when pH is increased from 3.8 to 6.8 but decreases at higher pHs of 8.3 and 9.8. Maximum antibody loading occurs at pH 6.8 ( Figure 2C), about midway between known neutral pH of alumina at ∼7.2 23 and the isoelectric point of rabbit anti-E. coli antibody at ∼pHs 6.1À6.5. 24 This trend compares favorably with a previously reported membrane-based virus biosensor using IgM antibody instead of IgG and indicates similar interactions between alumina surface and antibody which are largely electrostatic with negligible hydrophobic contributions.…”

Section: Resultsmentioning

confidence: 98%

Membrane-Based Electrochemical Nanobiosensor for Escherichia coli Detection and Analysis of Cells Viability

Cheng

Lau

Chow

et al. 2011

Environ. Sci. Technol.

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A sensitive and selective membrane-based electrochemical nanobiosensor is developed for specific quantitative label-free detection of Escherichia coli (E. coli) cells and analysis of viable but nonculturable (VBNC) E. coli cells which remain mostly undetected using current methods. The sensing mechanism relies on the blocking of nanochannels of a nanoporous alumina-membrane modified electrode, upon the formation of immune complexes at the nanoporous membrane. The resulting obstacle to diffusive mass transfer of a redox probe in the analysis solution to the underlying platinum electrode reduces the Faradaic signal response of the biosensor, measured using cyclic voltammetry. Antibody loading under conditions of varying antibody concentrations and pHs are optimized. The biosensor gives a low detection limit of 22 cfu mL(-1) (R(2) = 0.999) over a wide linear working range of 10 to 10(6) cfu mL(-1). It is specific toward E. coli with minimal cross-reactivity to two other pathogenic bacteria (commonly found in waters). Relative standard deviation (RSD) for triplicate measurements of 2.5% indicates reasonably useful level of reproducibility. Differentiation of live, VBNC, and dead cells are carried out after the cell capture and quantitation step, by simple monitoring of the cells' enzyme activity using the same redox probe in the analysis solution, in the presence of glucose.

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“…The pH of the prepared boehmite aqueous sol was 3.82, suggesting that the boehmite particles were positively charged (the pH at the point of zero charge, pH pzc lies between 6.3 and 8.4 25 ).…”

Section: The Effect Of Applied Voltage and Deposition Time On The Boementioning

confidence: 99%

“…25 Indeed, the dependence of the deposition current density on the deposition time at a constant applied voltage, 22 corresponded to the deposition of porous films. In addition, some hydrogen bubbles may remain trapped between the deposited sol particles, resulting, in both cases, in film porosity.…”

Section: The Effect Of Applied Voltage and Deposition Time On The Boementioning

confidence: 99%

Electrophoretic deposition and thermal treatment of boehmite coatings on titanium

Djosic

Mišković‐Stanković²,

Srdić

2007

JSCS

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An aqueous boehmite sol was prepared by the peptization of Al(OH) 3 . The electrophoretic deposition of boehmite coatings on titanium from the aqueous sol was performed at a constant voltage (from 1.0 to 10 V) and for a constant deposition time (from 10 to 30 min). Increasing the applied voltage and deposition time increased the mass of the boehmite coating. It was shown that boehmite coatings of maximum thickness, low porosity and good adhesion can be formed at lower deposition voltages and longer deposition times. The boehmite powder, obtained by drying the prepared aqueous sol, and the boehmite coatings were thermally treated at 1000°C and 1300°C with a holding period of 1 h at the maximum temperature. X-Ray diffraction analysis of the thermally treated samples confirmed the existence of g-Al 2 O 3 and a-Al 2 O 3 phases, respectively, while scanning electron microscopy revealed the graininess of the structure of the a-Al 2 O 3 coatings treated at 1300°C, indicating a significantly lower sintering temperature of the boehmite coating obtained by electrophoretic deposition.

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The Influence of the Solid/Liquid Ratio on the Point of Zero Charge of Alumina

Cited by 9 publications

References 24 publications

Membrane-Based Electrochemical Nanobiosensor for the Detection of Virus

Membrane-Based Electrochemical Nanobiosensor for the Detection of Virus

Membrane-Based Electrochemical Nanobiosensor for Escherichia coli Detection and Analysis of Cells Viability

Electrophoretic deposition and thermal treatment of boehmite coatings on titanium

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