The influence of the surface structure of polyaniline films on the adsorption of influenza A viruses and antibodies to them

Isakova, A. A.; Indenbom, A. V.; Yakobson, O. D.; Грибкова, О. Л.; Brevnov, V. V.; Гарина, Е. О.; Ванников, А. В.

doi:10.1134/s207020511604016x

Cited by 6 publications

(4 citation statements)

References 24 publications

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“…The first article dealing with antivirus activity of PANI dates back 2011 [173]. Even though it is not strictly related to biomedical applications it offers an interesting starting point demonstrating the ability of the material to remove influenza viruses A from wastewater [173][174][175]. More in detail, traditional, one-dimensional, CNT-coated and Ag modified polyanilines were tested as active materials in the adsorption of influenza viruses from aqueous solutions, proving to be more capable than carbon to bind different strains of viruses including infectious of A and B strains of human and avian viruses.…”

Section: Antivirus Activitymentioning

confidence: 99%

“…Doping anions able to improve the hydrophilicity of the polymer positively affect its adsorption properties promoting sorbent/water interactions. A greater effect is played by silver NPs that strongly influence the ability of PANI to interact with viruses participating actively to the process [175]. This ability of PANI and PANI composites was exploited by Isakova and coworkers to develop electrosynthesized PANI and PANI composites films to detect influenza viruses by surface plasmon resonance (SPR) [176].…”

Section: Antivirus Activitymentioning

confidence: 99%

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Advances in Polyaniline for Biomedical Applications

Pina

2022

CMC

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: Conducting polymers are an outstanding class of materials characterized by electroconducting properties that make them good candidates for applications in several sectors. Among them, polyaniline (PANI) is unique for its extraordinary ability to conduct electricity, biocompatibility and low toxicity. In spite of its surprising features, to date PANI have not yet found application in the practical uses, because of its low solubility and processability. In order to overcome these limitations, different approaches have been developed, such as polymer grafting processes, PANI-based composites and blends preparation. The present review describes the most recent advances on PANI applications in biomedical fields, such as antioxidant, antimicrobial and antivirus activity, drug delivery, cancer therapy, etc. A look to the synthetic procedures is also reported because it is crucial for the realization of more innovative materials in the next future.

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Section: Antivirus Activitymentioning

confidence: 99%

Section: Antivirus Activitymentioning

confidence: 99%

Advances in Polyaniline for Biomedical Applications

Pina

2022

CMC

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“…On the other hand, physical adsorption is a more popular strategy in which the antibody molecules are autonomously adsorbed on the substrate surface by the simple incubation process, without the need for complex operation protocols . In physical adsorption, the specific surface area of a substrate appears to be a critical factor that greatly affects the density and absolute amount of the immobilized antibody. ,, In order to increase the surface area, porous materials such as cellulose and nitrocellulose membranes and electrospun fiber mats are often employed as substrates. , These porous materials have the advantage of significantly high surface area; however, control of the micro/nanometer-scale geometries of these materials are not trivial, hence affecting the sensitivity and reliability of immunoassays. ,− In addition, there have been studies on the evaluation of antibody adsorption on precisely engineered structures. ,, To the best of our knowledge, however, lateral flow immunoassay systems, in which the surface roughness is precisely controlled by micro/nanofabrication techniques, have not been developed.…”

mentioning

confidence: 99%

“…14,22−24 In addition, there have been studies on the evaluation of antibody adsorption on precisely engineered structures. 19,20,25 To the best of our knowledge, however, lateral flow immunoassay systems, in which the surface roughness is precisely controlled by micro/nanofabrication techniques, have not been developed.…”

mentioning

confidence: 99%

Enhanced Immunoadsorption on Imprinted Polymeric Microstructures with Nanoengineered Surface Topography for Lateral Flow Immunoassay Systems

et al. 2019

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Lateral flow immunoassay devices have revolutionized the style of on-site disease detection and point-of-care testing in the past few decades. The surface nanotopography of a solid substrate is a dominant parameter in the efficiency of antibody immobilization, but precise control over surface roughness has not been fully investigated. Here we presented lateral flow immunoassay platforms with nanometer-scale surface roughness, reproducibly engineered using thermal nanoimprinting lithography, and investigated the effects of surface nanotopography on immunoadsorption and immunoassay performance. We fabricated three types of imprinted polycarbonate sheets with microcone array structures having different degrees of surface roughness using three types of molds fabricated by micromachining or laser ablation. The structures fabricated by laser-ablated nickel mold exhibited numerous bumps measuring several tens of nanometers, which enhanced antibody adsorption. We performed sandwich immunoassays of C-reactive protein in serum samples and achieved highly sensitive detection with a detection limit of ∼0.01 μg mL–1 and a broad dynamic range. The present results provide useful information on the remarkable effect of nanoengineered surfaces on biomolecule adsorption, and the platforms presented here will widen the applicability and versatility of lateral flow immunoassay devices.

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