The influence of theophylline and phenobarbital on rat brain 5′-nucleotidase

Jensen, Mogens H.; Jacobsen, Jan B.

doi:10.1111/j.1600-0404.1987.tb03542.x

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Studies showed a significant inhibition of rabbit renal 5Ј-nucleotidase (EC 3.1.3.5), cyclic nucleotide phosphodiesterase (EC 3.1.4.17), and adenosine deaminase (EC 3.5.4.4) by theophylline, but only at millimolar concentration (17). Furthermore, a significant inhibition of a 5Ј-nucleotidase from rat brain by different xanthine derivatives has been observed (18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Silva

Bruno

Battastini

et al. 2003

Neurochemical Research

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The psychostimulant caffeine promotes behavioral effects such as hyperlocomotion, anxiety, and disruption of sleep by blockade of adenosine receptors. The availability of extracellular adenosine depends on its release by transporters or by the extracellular ATP catabolism performed by the ecto-nucleotidase pathway. This study verified the effect of caffeine on NTPDase 1 (ATP diphosphohydrolase) and 5'-nucleotidase of synaptosomes from hippocampus and striatum of rats. Caffeine and theophylline tested in vitro were unable to modify nucleotide hydrolysis. Caffeine chronically administered in the drinking water at 0.3 g/L or 1 g/L for 14 days failed to affect nucleotide hydrolysis. However, acute administration of caffeine (30 mg/kg, i.p.) produced an enhancement of ATP (50%) and ADP (32%) hydrolysis in synaptosomes of hippocampus and striatum, respectively. This activation of ATP and ADP hydrolysis after acute treatment suggests a compensatory effect to increase adenosine levels and counteract the antagonist action of caffeine.

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Section: Introductionmentioning

confidence: 99%

Untitled

Silva

Bruno

Battastini

et al. 2003

Neurochemical Research

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“…Theophylline is known to be a nonspecific antagonist of P1 receptors, 1,2) and inhibitor of cyclic nucleotide phosphodiesterase, 2,10) 5-nucleotidase, 11,12) and alkaline phosphatase, 13) and a weak inhibitor of HGPT. 14,15) As previously reported, 4) theophylline had no effect on the Ado-induced ATP levels in PC12 cells without pretreatment.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Cellular Adenosine Triphosphate Levels in PC12 Cells by 2,5-Dideoxyadenosine, a P-Site Inhibitor of Adenylate Cyclase

Fujimori

Pan-Hou

2005

Biological & Pharmaceutical Bulletin

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To elucidate the biological significance of the P-site inhibitor of adenylate cyclase, the effect of 2,5-dideoxyadenosine (DDA) on cellular levels of adenine compounds in PC12 cells was studied. The addition of DDA and deoxyadenosine (deoxyAdo), P-site inhibitors of adenylate cyclase, as well as the addition of adenosine (Ado) to the incubation medium containing glucose as the sole nutrient significantly enhanced cellular ATP levels in PC12 cells. N 6 -Methyladenosine and N 6 -cyclohexyladenosine did not augment the ATP levels. The ATP level-enhancing effect of DDA was further enhanced by Ado. After pretreatment of PC12 cells with theophylline, DDAinduced ATP enhancement was potentiated by theophylline but the effect of Ado was suppressed. cAMP levels in PC12 cells were markedly reduced by DDA but the levels were not changed by Ado. These results suggest for the first time that P-site inhibitors of adenylate cyclase may stimulate ATP synthesis via glycolysis by decreasing cAMP levels and the mode of action of the ATP level-enhancing effect of DDA may be different from that of Ado.

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