The influence of Tween 80 on laccase production by Pleurotus sajor-caju and the efficiency of crude enzyme broth in the removal of bisphenol-A

Abstract

Bisphenol-A is currently considered an environmental pollutant, capable of interfering in the endocrine system of organisms and causing alterations in its development and reproductive system. An alternative method to the chemical treatment of this pollutant has been the use of oxidative enzymes, especially laccases produced by fungi. In order to reduce production costs, agro-industrial waste can be used in the culture medium composition. Nonionic surfactants, which are only slightly toxic to biological membranes, can be applied, as well as Tween 80, to facilitate the excretion of these enzymes into the culture medium. The objectives of this work were: a) characterize the immersion water of banana straw used in the formulation of the culture medium; b) evaluate laccase production by Pleurotus sajor-caju in culture medium with and without addition of Tween 80, through shaken flasks; c) evaluate the efficiency of the crude enzyme broth in degrading bisphenol-A. The shaken flasks were incubated at 30°C for 12 days. The immersion water had a C:N ratio of 13.8, ash percentage of 28.6%, and pH close to neutrality. The addition of Tween 80 on the culture medium (7.5%, m/v) yielded laccase activity and productivity values equal to 3,016.47 U L-1 and 502.7 U L-1 day-1, respectively. These values were 50 and 33.5 times higher than those obtained in the culture medium without addition of Tween 80 for laccase activity and productivity, respectively. The crude enzyme broth degraded 100% of bisphenol-A after 48 hours, regardless of concentration (500, 750 and 1,000 mg L-1).

Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

“…In addition, it was also found that Tween 80 was degraded by L. theobromae (Cao et al, 2020). Teodoro et al (2018) reported that the addition of Tween 80 to the culture medium enhanced laccase activity during the degradation of bisphenol A by Pleurotus sajor-caju. Nielsen et al (2016) found that the addition of Tween 80 (0.1%) to growth media increased the growth rate of Staphylococcus aureus batch cultures, and it also increased the total biomass when S. aureus was grown as biofilms.…”

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

“…In addition, It has been reported that laccase showed its maximum value at a Tween 80 concentration of 0.1% (Elsayed et al, 2012). Similarly, the addition of Tween 80 to the culture medium enhanced laccase activity during the degradation of bisphenol A by Pleurotus sajor-caju (Teodoro et al, 2018). In particular, Tween 80 has been shown to be a laccase inducer in Pleurotus eryngii grown in solid state fermentation (Akpinar & Ozturk, 2017).…”

Enhancement and analysis of Anthracene degradation by Tween 80 in LMS-HOBt

“…Furthermore, similar enhancements can also occur in the oxidation of other large molecular substrates, such as biodegradation of lignin by linoleic acid, arachidonic acid, and Tween 80 or Tween 20 36 – 39 , cellulose digestibility 40 , 41 and biobleaching hardwood kraft pulp 42 . Additionally, degradation of other organic pollutants can also be enhanced by Triton X-100, including indole 43 , bisphenol A 44 , 45 , phenol 46 – 48 , fungicide miconazole and antidepressant 49 , and asphalt 50 . As a result of lipid peroxidation reactions, the unstable α -position of ether oxygen bond (–C–O–C–) in Tween 20, carbon–carbon double bond (C=C) and that in Tween 80 should be also considered.…”